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| SN/T 2426-2010 | English | 519 |
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Determination of citrinin contents in cereals for import and export. HPLC method
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SN/T 2426-2010
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Basic data | Standard ID | SN/T 2426-2010 (SN/T2426-2010) | | Description (Translated English) | Determination of citrinin contents in cereals for import and export. HPLC method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X11 | | Classification of International Standard | 67.060 | | Word Count Estimation | 13,186 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Regulation (derived from) | National Quality Inspection (2010) No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import content in cereals Citrinin liquid chromatography test methods. This standard applies to import rice, barley, oats, wheat seed citrinin content. | SN/T 2426-2010: Determination of citrinin contents in cereals for import and export. HPLC method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of citrinin contents in cereals for import and export.HPLC method
Exit inspection and quarantine industry standard book People's Republic of China
Importers citrinin content in cereals Detection
Liquid chromatography
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Hubei Exit Inspection and Quarantine.
The main drafters of this standard. VETERINARY Zhao Asia, Hu Zhong, Wang Peng, Li Jing, Fu Xiaofang.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Importers citrinin content in cereals Detection
Liquid chromatography
1 Scope
This standard specifies the content of citrinin in cereals and export liquid chromatography detection methods.
This standard applies to detect the import and export of rice, barley, oats, wheat citrinin content.
2 Method summary
With acetonitrile - isopropyl alcohol - mixed solution of water extracted sample citrinin, C18 SPE column purification, distribution of liquid chromatography with fluorescence detector
Spectrometer, external standard.
3 Reagents and materials
Unless otherwise specified, reagents were of analytical grade, water is distilled water or equivalent purity.
3.1 Isopropanol. chromatography.
3.2 acetonitrile. chromatography.
3.3 phosphoric acid. pure class distinctions.
3.4 Extraction solvent. acetonitrile - isopropanol - water (35 + 10 + 55, by volume), adjusted to pH 1.5 with phosphoric acid.
3.5 phosphate solution. Take 5.6mL phosphoric acid to water volume to 1000mL.
3.6 Mobile phase. acetonitrile - isopropanol -0.08mol/L phosphoric acid (35 + 10 + 55, volume ratio).
3.7 C18 solid phase extraction column. 500mg, 3mL, or equivalent person. Before use, respectively 5mL methanol and 5mL water pre-rinse and keep cylinder
Moist.
3.8 Citrinin standard substance (citrinin, C13H14O5, CAS number. 518-75-2). purity greater than or equal to 97%.
3.9 Citrinin standard stock solution. weigh the amount of citrinin standard substance dissolved in acetonitrile and dilute to 1.0mg/mL, 0 ℃ ~ 4 ℃
save.
3.10 Citrinin standard working solution. dilute the standard stock solution with the necessary liquidity into with 25ng/mL, 50ng/mL, 100ng/mL,
500ng/mL, 1000ng/mL of standard working solution.
3.11 Glass fiber filter. diameter 11cm, pore size 1.5μm.
4 instruments and equipment
4.1 High performance liquid chromatograph. equipped with fluorescence detector.
4.2 oscillator.
4.3 Centrifuge. 4000r/min.
4.4 Solid phase extraction vacuum apparatus.
4.5 nitrogen blowing instrument.
4.6 Analytical balance.
5 Sample preparation and preservation
Take a representative sample 500g, crushed with a grinder and a circular hole through 830μm sieve, mix, divided into two, loaded into a clean container, dense
Seal and logo. In the sample preparation procedure, it should prevent sample contamination or the occurrence of the change of residue content.
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