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SN/T 2345-2009: The pine resin canker quarantine and identification methods
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2345-2009 | English | 319 |
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The pine resin canker quarantine and identification methods
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SN/T 2345-2009
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Basic data | Standard ID | SN/T 2345-2009 (SN/T2345-2009) | | Description (Translated English) | The pine resin canker quarantine and identification methods | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 12,144 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Regulation (derived from) | National Quality Inspection [2009] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the plant quarantine in the pine resin canker (Fusarium circinatum Nircnberg and O "Donnell) quarantine identification. This standard applies from pine resin canker in the countries and regions Pinus (Pinus spp.), Douglas fir is (Pseudotsuga spp.) and other pine resin canker host plants, seeds, seedlings and timber entry quarantine and identification. | SN/T 2345-2009: The pine resin canker quarantine and identification methods---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
The pine resin canker quarantine and identification methods
Exit inspection and quarantine industry standard book People's Republic of China
Pine resin canker quarantine and identification methods
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A, Appendix B, Appendix C, Appendix D and Appendix E are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted by the People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Li Baisheng, Liaotai Lin, Ji Rui, Wu Cuiping, Wu Xinhua, Ann Yulin.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Pine resin canker quarantine and identification methods
1 Scope
Identification methods.
spp. ) Pine resin canker and other host plants of quarantine and identification of entry of seeds, seedlings and timber.
Principle 2
2.1 taxonomic status
Fungi (Fungi), imperfect fungi Amon (Deuteromycotina), Trichosporon Gang (Hyphomycetes), tumor seat spore head (Tuberculariales),
2.2 Host
Body, cone, seed and soil-borne. National and regional distribution see Appendix A.
2.3 Identification of principle
Pine resin canker form (see Appendix B Figure B.1, Figure B.2), host range, transmission, Damage symptoms and molecular biology
Characteristics of the bacteria is the main basis for quarantine and identification of.
3 instruments, appliances and reagents
3.1 equipment, appliances
--- PCR instrument;
--- Gel electrophoresis equipment;
--- Centrifuge;
--- Vortex device;
--- Constant temperature water bath;
--- Gel imaging system;
--- Other conventional laboratory equipment, apparatus.
3.2 Reagents
Sucrose, dextrose, agar, peptone, potassium dihydrogen phosphate, magnesium sulfate (MgSO4 · 7H2O), quintozene (PCNB), Shigeru scattered kill
Agents, triadimefon, Ampicillin, rifampicin, Tris (Tris), cetyltrimethylammonium bromide (of CTAB), ethylenediaminetetraacetic
Acetic acid (EDTA), sodium hypochlorite, ethanol, proteinase K, phenol, chloroform, isoamyl alcohol, ammonium acetate (NH4Ac), magnesium chloride
(Of MgCl2) dimethyl sulfoxide (DMSO), dNTP, T Rao q DNA polymerase, ethidium bromide (the EB), liquid nitrogen.
4 identification of quarantine
4.1 Site Inspection
Check the wood, sheet metal or seedlings surface, with or without flow fat, ulcer plaque. With an ax, or a saw blade, respectively horizontal, vertical incision, observe timber
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