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SN/T 2342-2009: Quarantine identification of apple stem grooving virus
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Quarantine identification of apple stem grooving virus
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SN/T 2342-2009
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Basic data | Standard ID | SN/T 2342-2009 (SN/T2342-2009) | | Description (Translated English) | Quarantine identification of apple stem grooving virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 13,164 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Regulation (derived from) | National Quality Inspection [2009] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the plant quarantine in the apple stem grooving virus (Apple stem grooving virus) quarantine identification. This standard applies to apples, pears and other seedlings, other propagating material of apple stem grooving virus quarantine and identification. | SN/T 2342-2009: Quarantine identification of apple stem grooving virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine identification of apple stem grooving virus
Exit inspection and quarantine industry standard book People's Republic of China
Apple stem grooving virus quarantine and identification methods
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard appendix B, Appendices C and D are normative appendices, Appendix A is an information appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine, Ningbo Entry-Exit Inspection and Quarantine of People's Republic of China
Bureau, Chinese Academy of Inspection and Quarantine, Shihezi University, People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Black
Longjiang CIQ.
The main drafters of this standard. Guo Lixin, para military dimension to this spring, Liang Miao, Chen Yun, Chen Wenbing, Shaobi Ying, Deng Cong Liang, Liu Hongyi,
Zhu Shuifang.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Apple stem grooving virus quarantine and identification methods
1 Scope
This standard specifies the phytosanitary Apple stem grooving virus (Applestemgroovingvirus) of quarantine and identification methods.
This standard applies to seedlings of apples, pears, etc., quarantine and identification of other propagating material apple stem grooving virus.
Principle 2
2.1 scientific name and taxonomic status
Name. Applestemgroovingvirus
Abbreviation. ASGV
2.2 propagation mode
See Appendix A.
2.3 antigenic properties
Similar leaf spot virus (Applechloroticleafspotvirus) particle morphology, but are not related serologically.
2.4 viral genome
Genome sense single-stranded RNA 6496nt containing two ORF, ORF1 which encodes a 241kDa produces poly eggs
White, and then cut into a number of product features, the coat protein (CP) at the C-terminus; of ORF2 encoding produces a 36kDa protein movement (MP).
MP and CP genes of the virus gene is relatively conservative, and more conservative CP gene.
2.5 Identification based
According to Apple stem grooving virus with moderate immunogenicity, antiserum can be prepared virus, CP gene conservative and strong, and can be designed primers
Probes and other characteristics, double-antibody sandwich enzyme-linked immunosorbent assay, reverse transcription - polymerase chain reaction and real-time fluorescent RT-PCR reaction can be made
Work for the quarantine inspection and identification of the virus basis.
3 Reagents and solutions
Unless otherwise specified, all test reagents were analytical grade.
3.1 ELISA reagents. See Appendix B, Section B. Chapter 1.
3.2 The total RNA extraction reagent. TrizolReagent.
3.3 RT-PCR step reaction reagents. Ready-to-GoRT-PCRBeads.
3.4 chloroform.
3.5 isopropanol.
3.6 75% ethanol.
3.7 ethanol.
3.8 RNase-free water. double distilled water or commodities by 0.1% DEPC (diethylpyrocarbonate) treated RNase-free water.
3.9 reverse transcriptase and buffers.
3.10 ribonuclease inhibitor.
3.11 dNTPs. containing dATP, dCTP, dGTP, dTTP.
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