| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2343-2009 | English | 479 |
Add to Cart
|
3 days [Need to translate]
|
Quarantine identification of bean pod mottle virus
| Obsolete |
SN/T 2343-2009
|
PDF similar to SN/T 2343-2009
Basic data | Standard ID | SN/T 2343-2009 (SN/T2343-2009) | | Description (Translated English) | Quarantine identification of bean pod mottle virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 12,195 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Regulation (derived from) | National Quality Inspection [2009] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the bean English mottle virus quarantine procedures and methods of identification. This standard applies to England mottle virus may carry soy bean, beans and other seeds, seedlings and other plant propagating material quarantine and identification, but also for mediators insect quarantine and identification of the virus. | SN/T 2343-2009: Quarantine identification of bean pod mottle virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine identification of bean pod mottle virus
Exit inspection and quarantine industry standard book People's Republic of China
Bean pod mottle virus, quarantine and identification methods
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix B, Appendix C, Appendix D is a normative appendix, Appendix A is an information appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine Fujian People's Republic of China
Bureau, the Shenzhen People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Liao Furong, Chen Qing, Shen Jianguo, Zheng Yun, Lin Shiming, Chen Yun, GUO Qiong-xia, Huang Peng Ying, Wang Hongyi Wu Yuan,
Travel light source, Yu
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Bean pod mottle virus, quarantine and identification methods
1 Scope
This standard specifies the bean pod mottle virus quarantine procedures and identification methods.
This standard applies to identify and quarantine may carry soy bean pod mottle virus, beans and other seeds, seedlings and other plant propagation material, but also
Suitable mediator insect quarantine and identification of the virus.
Principle 2
2.1 taxonomic status
Bean pod mottle virus (Beanpodmottlevirus) belongs to the cowpea mosaic virus family (Comoviridae), cowpea mosaic virus genus
(Comovirus) members. It can be divided into sub-groups and sub-groups Ⅰ Ⅱ two subgroups. Related Information See Appendix A.
English abbreviation. BPMV
2.2 Serological Characteristics
BPMV has a strong immunogenicity, prepared polyclonal antibody can be detected soybean leaves, seeds, plants and herbs host mediator Queensland
Insect viruses.
2.3 Characteristics of Molecular Biology
BPMV is a positive-strand RNA viruses whose genome is composed of two positive-stranded RNA-1 (about 3.6kb) and RNA-2 (about 6.0kb) group
As it was wrapped in two diameters of 28nm isometric polyhedron mitochondria. RNA-1 encoding five proteins required for replication, RNA-2 series
Inter-code a protein derived cell motility and two coat protein. According to its genomic sequence can be designed for molecular detection of specific primers
Primers and probes for specific detection of the virus.
Kinds of criteria for the classification BPMV where cowpea mosaic virus genus are.
a) a coat protein (Coatprotein, CP) amino acid sequence homology of less than 75%;
b) polymerizing the amino acid sequence of protease homology less than 75%;
c) between the components may not leave the reorganization;
d) antigenic response are different.
3 Main equipment and reagents
3.1 The main equipment and appliances
--- Trace Mills;
--- Microplate reader;
--- Washer;
--- Microbalance (sense of volume. 0.001g);
--- PCR instrument;
--- Electrophoresis;
--- Horizontal electrophoresis tank;
--- Gel imager;
--- Desktop high-speed refrigerated centrifuge;
--- Water bath;
--- PH meter;
--- Various measurement ranges adjustable pipette (1000μL, 200μL, 20μL, 2μL);
|