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SN/T 2329-2009: Cosmetics ocular irritant and corrosive HET-CAM test
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2329-2009 | English | 289 |
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Cosmetics ocular irritant and corrosive HET-CAM test
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SN/T 2329-2009
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Basic data | Standard ID | SN/T 2329-2009 (SN/T2329-2009) | | Description (Translated English) | Cosmetics ocular irritant and corrosive HET-CAM test | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 11,143 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Regulation (derived from) | Industry standard filing Notice 2009 No. 10 (No. 118 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the cosmetic eye irritation/corrosion test chorioallantoic membrane of the basic principle, test preparation, test methods, results and reports. This standard applies to cosmetics, cosmetic ingredients/cosmetic eye irritation/corrosion effects screening and detection. This standard toxicity tests can be used as part of combination with other alternative tests and animal testing for eye irritation test substance overall. This standard can also be used as pharmaceutical, agricultural and industrial chemicals and pollutants reference method for the determination of toxicity. | SN/T 2329-2009: Cosmetics ocular irritant and corrosive HET-CAM test---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Cosmetics ocular irritant and corrosive HET-CAM test
Exit inspection and quarantine industry standard book People's Republic of China
Cosmetic eye irritation/corrosion
Chick chorioallantoic membrane assay
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A and Appendix B are normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Entry Inspection and Quarantine Bureau, People's Republic of China Beijing Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Pan Fang, Cheng Shujun, Xuchong Hui, Jiao Hong, Wenqiao Ling, Shi Xiju, Liu Huizhi.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Cosmetic eye irritation/corrosion
Chick chorioallantoic membrane assay
1 Scope
This standard specifies the cosmetic eye irritation/corrosion of the basic principles of chick embryo capsule test, test preparation, test methods, results and
report.
This standard applies to cosmetics, screening and testing cosmetic ingredients/chemicals Eye irritation/corrosion effect.
This standard can be used as part of toxicity testing, in conjunction with other alternative tests and test animals were tested for eye irritation overall rating.
This standard can also be used as a reference method for the pharmaceutical, agricultural and industrial chemicals and toxic pollutants measured.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
In and around the eye after anterior direct contact with the test substance causes reversible inflammatory changes of the mucosa.
2.2
Eyes and mucous membranes around the eyes after direct contact test substances cause irreversible tissue damage.
2.3
Embryo age 4d ~ 5d, the body wall by the chorionic mesoderm and urine capsule visceral mesoderm fused together, the histological structure has three layers.
2.4
CAM vascular blood flow from the inner membrane blood vessels, can be represented as a flocculent or spotting appears extravascular diffuse bleeding and other
form.
2.5
Intravascular and extravascular denatured protein, manifested as intravascular thrombosis or blood flow slows, blood vessels showing brown-black, with mixed extravascular
Turbid and opaque.
2.6
CAM membrane wall rupture of small blood vessels, small blood vessels melting away.
3 Fundamentals
Chicken chorioallantoic membrane assay was assessed in vitro method for eye irritation earlier adopted, chorioallantoic membrane (CAM) is a breath
Film, surrounded by chicken around. In this experiment, the mid-hatched chick chorioallantoic membrane vascular system complete, clear and transparent characteristics of the
A certain amount of test substance directly chorioallantoic membrane contact, observe the effect of a period of time after allantocherion toxic effect indicators (such as. bleeding, blood clotting
Changes in blood vessels and melting) changes in morphology of these indicators reflect the vessels and vascular network, color and permeability, as well as reflect the chorioallantoic
Membrane protein denaturation phenomenon and the extent of damage and then combined to give a score for the assessment of eye irritation test substance. The purpose of the present experiment
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