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SN/T 2328-2009: Cosmetics acute toxicity of keratinocyte cytotoxicity test
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2328-2009 | English | 469 |
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Cosmetics acute toxicity of keratinocyte cytotoxicity test
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SN/T 2328-2009
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Basic data | Standard ID | SN/T 2328-2009 (SN/T2328-2009) | | Description (Translated English) | Cosmetics acute toxicity of keratinocyte cytotoxicity test | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 18,129 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Regulation (derived from) | Industry standard filing Notice 2009 No. 10 (No. 118 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the cosmetics acute toxicity test method keratinocytes basic principle, test preparation, test procedures, results and reports. This standard applies to cosmetic raw materials and finished products cytotoxicity screening and detection. This standard applies to predict rodent acute oral toxicity test starting dose, toxicity tests can be used as part of combination with other tests used to evaluate the overall toxicity of the test substance. This standard can also be used as pharmaceutical, agricultural and industrial test substances, food additives and contact materials, and other reference method for measuring the toxicity of pollutants. | SN/T 2328-2009: Cosmetics acute toxicity of keratinocyte cytotoxicity test---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Cosmetics acute toxicity of keratinocyte cytotoxicity test
Exit inspection and quarantine industry standard book People's Republic of China
Cosmetics acute toxicity test keratinocytes
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A, Appendix B and Appendix D is a normative appendix Appendix C are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Shanghai People's Republic of China
Bureau, People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. Cheng Shujun, Xuchong Hui, Jiao Hong, Wenqiao Ling, Qin Yao, Qiu Lu Zheng Moon.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Cosmetics acute toxicity test keratinocytes
1 Scope
This standard specifies the basic principles of cosmetic acute toxicity test method keratinocytes, test preparation, test procedures, results and reports.
This standard applies to raw materials and finished cosmetic cytotoxicity screening and detection.
This standard applies to predict rodent acute oral toxicity test starting dose can be used as part of toxicity testing, combined with other
Test is used to test substance toxicity overall rating.
This standard cytotoxicity assay can also be used as pharmaceutical, agricultural and industrial test substance, food additives and contact materials, and other contaminants
Reference method.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
About the test substance and the concentration-response relationship of S-type contains four variables logarithm mathematical model.
Y = Bottom + Top-Bottom1 + 10 (logIC50-X) HilSlope
Y is the response, X is the dose (or concentration) of the number, Bottom is the minimum response, Top is the maximum response, logIC50 dose or concentration of the reaction
Logarithmic, HilSlope represents the inclination of the curve at the highest (Top) and lowest (Bottom) when the middle.
2.2
Human epidermal keratinocytes constitute the main body of the cell, it is a kind of synthesis of keratin cells in vivo growth was layered arrangement; vitro points
Keratinocytes derived from foreskin or chest and abdomen, etc. skin tissue.
2.3
20% lethal concentration IC20
Under certain conditions HKC inhibiting the growth and viability of cells of 20% of the test concentration.
2.4
Median lethal concentration (IC50)
Under certain conditions HKC cell growth and viability of the 50% inhibition concentration of the test substance.
2.5
80% lethal concentration IC80
Under certain conditions HKC inhibiting the growth and viability of cells 80% of the test concentration.
3 Fundamentals
This standard uses normal human skin cells, cytotoxic application neutral red uptake (NRU) cytotoxicity test for detection of the test substance. in vitro
Cultured mammalian cells are constantly dividing normal proliferation, toxic substances regardless of their site of action and mechanism will interfere with cell division
Proliferation, resulting in decreased cell growth rate and reduce the number. Normal cells in culture were subjected to the test after exposure, it showed cytotoxic concentration
Dependence of neutral red uptake decreased, thus can be drawn about the integrity of the damaged cells and growth suppression effect of information. Neutral red
(NR) is a weak cationic dye, non-ionic diffusion can easily penetrate the cell membrane and accumulates in the lysosome. Living cells
Combined with neutral red dye and connection features. Lysosomal membrane change or exogenous substances on the surface of the cell membrane caused by sensitivity to change,
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