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SN/T 2178-2008: Test method of in vivo mammalian erythrocyte micronucleus for dangerous goods
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2178-2008 | English | 154 |
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Test method of in vivo mammalian erythrocyte micronucleus for dangerous goods
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SN/T 2178-2008
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Basic data | Standard ID | SN/T 2178-2008 (SN/T2178-2008) | | Description (Translated English) | Test method of in vivo mammalian erythrocyte micronucleus for dangerous goods | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C70 | | Classification of International Standard | 13.300 | | Word Count Estimation | 6,671 | | Date of Issue | 2008-09-04 | | Date of Implementation | 2009-03-16 | | Quoted Standard | GB 14924.1; GB 14925; Ministry of Health, "Disinfection technical specifications" | | Adopted Standard | OECD NO.474-1997, MOD | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the mammalian bone marrow cell micronucleus test terminology and definitions, reagents and instruments, test methods and test results. This standard applies to detect dangerous chemicals seized mutagenic effect. | SN/T 2178-2008: Test method of in vivo mammalian erythrocyte micronucleus for dangerous goods---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method of in vivo mammalian erythrocyte micronucleus for dangerous goods
Exit inspection and quarantine industry standard book People's Republic of China
Dangerous mammal erythrocytes
Micronucleus test methods
Posted 2008-09-04
2009-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This revised standard adopts the United Nations Organisation for Economic Co-operation and Development (OECD) Chemicals Test Method No. 474 "mammalian erythrocyte micronucleus
Nuclear-Test "(1997).
This standard OECD Chemicals Test Method No. 474 compared with the following differences.
--- To OECD Chemicals Test Method No. 474 were editorial changes;
--- Increased the preamble;
--- Increased reagent and instrument content.
This standard was proposed by the State Administration of Quality Supervision Inspection and Quarantine of People's Republic of China.
This standard by the National Certification and Accreditation Administration Committee.
This standard is drafted by. People's Republic of China Tianjin Exit Inspection and Quarantine.
Participated in the drafting of this standard. the Chemical Institute of Standardization.
The main drafters. Zhang Yuan Zhao good Lippo, in Rui Zhi, Zhao Zhuo, Xiongzhong Jiang, Li Xue Yang.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Dangerous mammal erythrocytes
Micronucleus test methods
1 Scope
This standard specifies the mammal PCE terms and definitions cell micronucleus test, reagents and instruments, test methods and test
result.
This standard applies to detect dangerous chemicals mutagenic effect.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB 14924.1 animal feed common quality standards
GB 14925 Laboratory Animal Facilities and Environment
The Ministry of Health "disinfection technical specifications"
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
In late mitotic cell division, chromosomes into daughter cells regularly formed the nucleus, is still stuck in the cytoplasm of a single dye
No body kinetochore or chromosome fragment, or spindle damage and loss due to whole chromosome. At the end of it after the formation of a single or a few
Nuclear rules, and is contained within the cytoplasm of the daughter cells is formed by a small nucleus than the main, it is called micronuclei.
3.2
Addicted containing micronuclei polychromatic erythrocytes number of parts per thousand (‰) indicates.
3.3
Cell metaphase chromosomes attached to spindle fibers area, whereby the offspring chromosomes to regularly move the poles to daughter cells.
3.4
Mature red blood cells, has disappeared because of the ribosome, by selective staining of immature erythrocytes distinguished.
3.5
Immature red blood cells, red blood cell maturation is an intermediate stage, this time due to the cytoplasm still contains ribosomes, through selective
Staining with the mature red blood cells to distinguish.
4 Reagents and Instruments
4.1 Reagents
Unless otherwise indicated, all the reagents, were of analytical grade, test water is distilled water.
4.1.1 calf serum (inactivated). preparation methods, see "disinfection technical specifications."
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