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SN/T 2160-2008: Determination of prednisolone residues in animal food-GC-MS/MS method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2160-2008 | English | 209 |
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Determination of prednisolone residues in animal food-GC-MS/MS method
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SN/T 2160-2008
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Basic data | Standard ID | SN/T 2160-2008 (SN/T2160-2008) | | Description (Translated English) | Determination of prednisolone residues in animal food-GC-MS/MS method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53;X22 | | Classification of International Standard | 67.050; 67.120.01 | | Word Count Estimation | 8,823 | | Date of Issue | 2008-09-04 | | Date of Implementation | 2009-03-16 | | Regulation (derived from) | Industry standard filing Notice No. 11 of 2008 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the food of animal origin prednisolone residue detection method. This standard applies to food of animal origin prednisolone residues detected. | SN/T 2160-2008: Determination of prednisolone residues in animal food-GC-MS/MS method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of prednisolone residues in animal food-GC-MS/MS method
Exit inspection and quarantine industry standard book People's Republic of China
Animal Food prednisolone Residue Detection
Gas chromatography - mass spectrometry/mass spectrometry
Posted 2008-09-04
2009-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
The standard by the China Academy of Inspection and Quarantine is responsible for drafting.
The main drafters of this standard. Jones Lang Vico, Ding Gang fight, Li Xiang, Li Li, Liu Hanxia, Zhang creel, Sun Yi's.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Animal Food prednisolone Residue Detection
Gas chromatography - mass spectrometry/mass spectrometry
1 Scope
This standard specifies the method for detection of food of animal origin prednisolone residues.
This standard applies to food of animal origin prednisolone residues detected.
2 Determination
2.1 Method summary
Samples were extracted with acetonitrile by C18 solid-phase extraction (SPE), prednisolone purified under acidic conditions rapid oxidation with K2Cr2O7
Derivative, gas chromatography - tandem mass spectrometry (GC-MS2) detection, external standard.
2.2 Reagents and materials
2.2.1 Methanol (HPLC grade).
2.2.2 acetonitrile (HPLC grade).
2.2.3 acetone (HPLC grade).
2.2.4 n-hexane (HPLC grade).
2.2.5 dichloromethane (HPLC grade).
2.2.6 ethyl acetate (HPLC grade).
2.2.7 ethanol (HPLC grade).
2.2.8 Standard prednisolone (prednisolone, CAS #. 50-24-8, molecular formula C21H28O5). purity ≥99%.
2.2.9 prednisolone standard stock solution. 10mg/L of anhydrous methanol.
2.2.10 acetic acid - sodium acetate buffer solution (pH4.6, was added 42mL in 158mL acetic acid solution of sodium acetate).
2.2.11 acidic K2Cr2O7 solution (18mL take water and add 2mL of concentrated sulfuric acid, plus 1.0gK2Cr2O7).
2.2.12 hydrolysis juice (including β- glucuronidase glycosidase and esterase sulfur, greater than ≥10000000μ/g).
2.2.13 C18 solid phase extraction column (3mL, 500mg).
2.2.14 sodium bicarbonate solution (10%).
2.3 instruments and equipment
2.3.1 Gas chromatography - ion mass spectrometry (EI source).
2.3.2 Nitrogen blowing instrument.
2.3.3 High-speed centrifuge.
2.3.4 oscillator.
2.3.5 Vortex.
2.3.6 Solid phase extraction device.
2.3.7 ultrasonic cleaner.
2.4 Determination of step
2.4.1 Extraction
Weigh 10.00g tissue samples (pork, pig liver and pig kidney) tissue masher pulverized into a paste, placed in a 50mL centrifuge tube, add
15mLpH4.6 into acetic acid - sodium acetate buffer solution, then add 200μL enzyme juice, shaking 10min, ultrasonic 15min after 60 ℃
Water bath 2h. After cooling, 20mL acetonitrile and shaken for 30min, to 7500r/min centrifugation 20min, the upper acetonitrile
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