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SN/T 2143-2008: Determination of cryptosporidium in food for import and export. PCR method
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2143-2008 | English | 239 |
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Determination of cryptosporidium in food for import and export. PCR method
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SN/T 2143-2008
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Basic data | Standard ID | SN/T 2143-2008 (SN/T2143-2008) | | Description (Translated English) | Determination of cryptosporidium in food for import and export. PCR method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Word Count Estimation | 9,987 | | Date of Issue | 2008-09-04 | | Date of Implementation | 2009-03-16 | | Quoted Standard | GB/T 5750.12; GB/T 6682; SN/T 1193 | | Regulation (derived from) | Industry standard filing Notice No. 11 of 2008 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the food Cryptosporidium PCR testing methods. This standard applies to fruit juice, milk, leafy vegetables and fruits, shellfish in Cryptosporidium rapid test. | SN/T 2143-2008: Determination of cryptosporidium in food for import and export. PCR method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of cryptosporidium in food for import and export.PCR method
Exit inspection and quarantine industry standard book People's Republic of China
Import and export food Cryptosporidium detection methods
PCR Method
Posted 2008-09-04
2009-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Li Xiaohong, Toray Yan, Han Wei.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export food Cryptosporidium detection methods
PCR Method
1 Scope
This standard specifies the foods Cryptosporidium PCR test.
This standard applies to quick check of juice, milk, leafy vegetables and fruits, shellfish in Cryptosporidium.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 5750.12 drinking water standard test methods of microbiological criteria
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987)
SN/T 1193 genetic testing laboratory technical requirements
3 definitions, terms and abbreviations
The following terms, definitions and abbreviations apply to this standard.
3.1 Definitions and Terminology
3.1.1
Cryptosporidium is top composite doors, Cryptosporidium classes, subclasses coccidia, coccidia really mesh, suborder Eimeria, Cryptosporidium Branch, Cryptosporidium
Spp. In diameter (3.2μm ~ 5.1μm) × (8.0μm ~ 8.5μm) of tiny, to the naked eye can not recognize a single-celled parasite.
Cryptosporidium parasites in the human or animal gastrointestinal epithelium and airway epithelial microvilli, which Cryptosporidiosis is an important zoonotic send
Health sis. Foodborne infections through sexual transmission of infections or water. Currently recognized species of Cryptosporidium effective 13 kinds. Wherein sense
3.2 Acronyms
3.2.1
MACS separation.
3.2.2
Restriction fragment length polymorphism analysis.
4 Equipment and Materials
4.1 Electronic balance. a sense of the amount of 0.001g.
4.2 Microscopy. × 100 times.
4.3 Centrifuge. 3000r/min.
4.4 temperature water bath. 36 ℃ ± 1 ℃, 65 ℃ ± 1 ℃.
4.5 PCR amplification.
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