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SN/T 2110-2008: Determination of 2-amino-4-nitrophenol and 2-amino-5-nitrophenol in hair dye for import and export
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2110-2008 | English | 239 |
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Determination of 2-amino-4-nitrophenol and 2-amino-5-nitrophenol in hair dye for import and export
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SN/T 2110-2008
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PDF similar to SN/T 2110-2008
Basic data | Standard ID | SN/T 2110-2008 (SN/T2110-2008) | | Description (Translated English) | Determination of 2-amino-4-nitrophenol and 2-amino-5-nitrophenol in hair dye for import and export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Word Count Estimation | 9,953 | | Date of Issue | 2008-07-17 | | Date of Implementation | 2009-02-01 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 9 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the oxidative hair dye, 2 amino-4 nitrophenol and 2 amino 5 nitro phenol liquid chromatographic method. This standard applies to oxidative hair dye, 2 amino-4 nitrophenol and 2 amino 5 nitro phenol determination. | SN/T 2110-2008: Determination of 2-amino-4-nitrophenol and 2-amino-5-nitrophenol in hair dye for import and export
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Determination of 2-amino-4-nitrophenol and 2-amino-5-nitrophenol in hair dye for import and export
Exit inspection and quarantine industry standard book People's Republic of China
Importers hair dye and 2-amino-4-nitrophenol
Determination of 2-amino-5-nitro phenol
Posted 2008-07-17
2009-02-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A, Appendix B are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Yu Wen Lin, Chen Wei, Zhang Qing, Zhou Xin, birch, Wang Chao.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Importers hair dye and 2-amino-4-nitrophenol
Determination of 2-amino-5-nitro phenol
1 Scope
This standard specifies the method of liquid chromatography-oxidative hair dye 2-amino-4-nitrophenol and 2-amino-5-nitro phenol.
This standard applies to oxidative hair dye in 2-amino-4-nitrophenol was measured and 2-amino-5-nitro phenol.
Principle 2
Oxidation Hair 2-amino-4-nitrophenol and 2-amino-5-nitrophenol ultrasonic extraction with methanol, centrifuged and the supernatant was filtered, mining
By liquid chromatograph equipped with a UV detector. According to their retention time qualitative, external standard, liquid chromatography - mass spectrometry confirmed.
3 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water for ultra-pure water.
3.1 Methanol. HPLC fractions.
3.2 25mmol/L potassium dihydrogen phosphate solution (pH = 7.0). Weigh 3.7g potassium dihydrogen phosphate, diluted with water to 1000mL, with 10% hydrogen
Hydroxide solution to adjust the pH to 7.0.
3.3 Standard. 2-amino-4-nitrophenol (CAS99-57-0). purity ≥99.9%; 2- amino-5-nitro-phenol (CAS121-88-0). Pure
Of ≥90%.
3.4 standard stock solution (500mg/L). accurately weighed amount of 2-amino-4-nitrophenol and 2-amino-5-nitrophenol (accurate to
0.1mg), methanol concentration was formulated as 500mg/L standard stock solution. Frozen 2 weeks from light use.
3.5 standard solution. Pipette appropriate amount of standard stock solution required, diluted to standard working solution with a suitable concentration of methanol, the same day with
System, using brown volumetric flask.
4 instruments and equipment
4.1 liquid chromatograph. equipped with a UV detector.
4.2 Liquid chromatography - tandem mass spectrometry.
4.3 ultrasonic cleaner.
4.4 high-speed centrifuges.
4.5 Vortex.
5 measuring step
5.1 sample handling
Weigh accurately Oxidation Hair dye approximately 0.5g (accurate to 0.001g), placed in 25mL brown stoppered centrifuge tube, add armor
Alcohol about 20mL, after mixing in a vortex, ultrasonic cleaner ultrasonic extraction 10min, transferred to 25mL brown volumetric flask, with methyl
Alcohol to volume. A portion of the solution into a 10mL stoppered centrifuge tube, 12000r/min centrifugal 10min, the supernatant through
0.45μm microporous membrane filtration, the filtrate for the machine measurement.
5.2 Determination
5.2.1 liquid chromatography conditions
5.2.1.1 Column. ZORBAXEclipsePlusC18,5μm, 4.6mm (inner diameter) × 250mm or equivalent person.
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