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Determination of dicumarol and cyclocoumarol in cosmetics for import and export. Liquid chromatography
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SN/T 2104-2008
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Basic data | Standard ID | SN/T 2104-2008 (SN/T2104-2008) | | Description (Translated English) | Determination of dicumarol and cyclocoumarol in cosmetics for import and export. Liquid chromatography | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Word Count Estimation | 7,735 | | Date of Issue | 2008-07-17 | | Date of Implementation | 2009-02-01 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 9 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of cosmetics and ring coumarin dicoumarol liquid chromatographic method. This standard applies to skin care category, skin cleansing category, skin care/cosmetics import and export of dual-modified class ring coumarin and coumarin determination. | SN/T 2104-2008: Determination of dicumarol and cyclocoumarol in cosmetics for import and export. Liquid chromatography
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Determination of dicumarol and cyclocoumarol in cosmetics for import and export.Liquid chromatography
Exit inspection and quarantine industry standard book People's Republic of China
Determination of the import and export of cosmetics double coumarin and coumarin ring
Liquid chromatography
Posted 2008-07-17
2009-02-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
The standard by the China Academy of Inspection and Quarantine is responsible for drafting.
The main drafters of this standard. Cheng Yan, Wang Xing, Wu Ting, Chen Wei, Linyuan Hui, Zhang Fan, Liu Liu, Wang Chao.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Determination of the import and export of cosmetics double coumarin and coumarin ring
Liquid chromatography
1 Scope
This standard specifies the import and export cosmetics dicoumarin liquid chromatography method and ring coumarin.
This standard applies to skin care category, skin cleaning category, skin care/cosmetics import and export modified class double coumarin and coumarin ring
Determination.
Principle 2
Cosmetics double coumarin and coumarin ring with acetonitrile + 0.1mol/L sodium hydroxide solution (9 + 1, volume ratio) ultrasonic extraction, filtration
After RP-HPLC separation technique to measure. According to their retention time qualitative, external standard.
3 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade and water as secondary deionized water.
3.1 acetonitrile chromatography.
3.2 methanol, pure class distinctions.
3.3 pairs of coumarin purity of ≥99%.
3.4 coumarin ring, purity ≥99%.
3.5 0.1mol/L sodium hydroxide solution. Weigh 0.40g of sodium hydroxide, dissolved in water after the transfer to a 100mL volumetric flask, dilute to the mark.
3.6 1.0mol/L acetic acid solution. Pipette 5.7mL of glacial acetic acid dissolved in water after the transfer to a 100mL volumetric flask, dilute to the mark.
3.7 0.05mol/L ammonium acetate solution (containing 0.25% by volume of acetic acid). weigh 3.85g of ammonium acetate, dissolved in water after transferred to
1000mL flask, add 2.5mL of glacial acetic acid, the volume filtered reserve.
3.8 pairs of coumarin and coumarin ring stock standard solution (100mg/L). Weigh accurately dicoumarin respectively (3.3) and coumarin ring (3.4)
0.01g, accurate to 0.0001g, was added 5mL of dichloromethane, was added 5mL of acetonitrile (3.1), all dissolved solids sonication with acetonitrile
Constant volume to 100mL, preserve refrigerator. Diluted with acetonitrile required to apply a standard working solution concentration, using now.
Acetonitrile + 3.9 Sodium hydroxide solution. amount of acetonitrile (3.1) 90mL, was added 0.1mol/L sodium hydroxide solution (3.5) 10mL, was mixed
Uniform, formulated as a 100mL solution.
4 Instrument
4.1 liquid chromatograph. equipped with a diode array detector.
4.2 micro-injector. 10μL.
4.3 ultrasonic cleaner.
Centrifuge 4.4. more than 6000r/min.
4.5 and 0.45μm organic solvent filter filtration membranes.
5 measuring step
5.1 Sample Processing
Weigh 0.5g sample cosmetics teflon container to the nearest 0.001g, acetonitrile + sodium hydroxide solution (3.9)
15mL, in an ultrasonic cleaner ultrasonic vibration 20min, a solution of 0.15mL acetic acid solution (3.6), and sodium hydroxide solution using acetonitrile +
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