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SN/T 2099-2008: Determination of Pseudomonas aeruginosa in food for import and export
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2099-2008 | English | 319 |
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Determination of Pseudomonas aeruginosa in food for import and export
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SN/T 2099-2008
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Basic data | Standard ID | SN/T 2099-2008 (SN/T2099-2008) | | Description (Translated English) | Determination of Pseudomonas aeruginosa in food for import and export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Word Count Estimation | 12,195 | | Date of Issue | 2008-07-17 | | Date of Implementation | 2009-02-01 | | Quoted Standard | GB/T 4789.28-2003; GB/T 6682 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 9 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the food Pseudomonas aeruginosa qualitative and quantitative detection method. This standard applies to all types of food detection of Pseudomonas aeruginosa, can refer to this method to test water Pseudomonas aeruginosa. | SN/T 2099-2008: Determination of Pseudomonas aeruginosa in food for import and export---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of Pseudomonas aeruginosa in food for import and export
Exit inspection and quarantine industry standard book People's Republic of China
Import and export food Pseudomonas aeruginosa Detection
Posted 2008-07-17
2009-02-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A, Appendix B are normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Zheng Jing, Huang Xiaorong, Zhao Guiming, Weng Guozhu, Wu Yunyun, Zheng Linyi, Chen Bin, Shao Biying.
This standard entry-exit inspection and quarantine standards was first published.
Import and export food Pseudomonas aeruginosa Detection
1 Scope
This standard specifies the method for qualitative and quantitative detection of Pseudomonas aeruginosa in food.
This standard applies to all types of food in the detection of Pseudomonas aeruginosa, can refer to this method to detect water Pseudomonas aeruginosa.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 4789.28-2003 Microbiological examination of food staining, media and reagents
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Also called Pseudomonas aeruginosa, a capsule, flagella, Gram-negative bacteria spore-free, able to break down proteins, carbohydrates ferment capacity is low,
Water-soluble pigments can produce two kinds. one is pyocyanin, blue-green phenazine compounds, no fluorescence, another is fluorescein, and green.
4 Equipment and Materials
4.1 incubator. 36 ℃ ± 1 ℃, 42 ℃.
4.2 Microscope. Lens with oil.
4.3 homogenizer.
4.4 autoclave.
4.5 Balance. Accuracy 0.1g.
4.6 VITEK automated microbial identification system, or similar device.
NOTE. VITEK is the trade name of the product supplied by the French company bioMérieux. This information is given for the convenience of users of this standard does not mean that
Endorsement of the product. If other equivalent product has the same effect, you can use these equivalent products.
4.7 straw. 1mL, sub-scale 0.1mL; 10mL, sub-scale 1mL.
4.8 Adjustable Pipette. 10μL ~ 100μL, 100μL ~ 1000μL.
4.9 tube. 15mm × 100mm.
4.10 sterilized dishes. diameter 90mm, bottom flat glass or plastic disposable sterile petri dish.
4.11 sterilized sample preparation equipment. tweezers, scissors, spoons.
4.12 quality control strains. Pseudomonas aeruginosa standard strain ATCC9027, Pseudomonas putida ATCC49128 or other has proven
The same biological characteristics similar strains.
5 medium and reagents
Unless otherwise specified, the reagents were of analytical grade, test water shall comply with GB/T 6682's.
5.1 SCDLP enrichment broth. see Section A. Chapter 1.
5.2 phosphate buffer dilution. see section A. 2.
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