|
US$209.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 1961.13-2013: Detection of allergen components in food for export. Part 13: Real time PCR method for detecting wheat components
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1961.13-2013 | English | 209 |
Add to Cart
|
3 days [Need to translate]
|
Detection of allergen components in food for export. Part 13: Real time PCR method for detecting wheat components
| Valid |
SN/T 1961.13-2013
|
PDF similar to SN/T 1961.13-2013
Basic data | Standard ID | SN/T 1961.13-2013 (SN/T1961.13-2013) | | Description (Translated English) | Detection of allergen components in food for export. Part 13: Real time PCR method for detecting wheat components | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 8,849 | | Quoted Standard | GB/T 6682; GB/T 27403 | | Regulation (derived from) | AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the food allergen wheat ingredients real-time PCR detection method. This standard applies to food and raw materials in the qualitative detection of raw wheat allergy component. The standard multi-minimum detection limit (LOD) of 0. | SN/T 1961.13-2013: Detection of allergen components in food for export. Part 13: Real time PCR method for detecting wheat components
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of allergen components in food for export.Part 13. Real time PCR method for detecting wheat components
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Export of food allergens detection
Part 13. real-time PCR method
Detection wheat ingredient
Part 13. RealtimePCRmethodfordetectingwheatcomponents
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1961 "export of food allergens detection" is divided into 19 parts.
--- Part 1. enzyme-linked immunosorbent assay peanut ingredients;
--- Part 2. real-time PCR was used to detect peanut ingredients;
--- Part 3. enzyme-linked immunosorbent assay buckwheat protein component;
--- Part 4. real-time PCR method for detection of cashew component;
--- Part 5. real-time PCR method to detect pistachios ingredients;
--- Part 6. real-time PCR method to detect walnut ingredients;
--- Part 7. real-time PCR method to detect carrot ingredient;
--- Part 8. real-time PCR method for detecting hazelnut ingredients;
--- Part 9. real-time PCR method for detection of almond ingredients;
--- Part 10. real-time PCR method to detect shrimp/crab ingredients;
--- Part 11. real-time PCR method to detect gluten ingredients;
--- Part 12. real-time PCR method for detection of sesame ingredients;
--- Part 13. real-time PCR method to detect wheat component;
--- Part 14. real-time PCR method to detect fish ingredients;
--- Part 15. real-time PCR method for detection of celery ingredients;
--- Part 16. real-time PCR method for detection of mustard ingredients;
--- Part 17. real-time PCR method for detection of lupine ingredient;
--- Part 18. real-time PCR method for detecting buckwheat ingredients;
--- Part 19. real-time PCR method to detect soy ingredients.
This section SN/T 1961 Part 13.
This section drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section drafted by. China Inspection and Quarantine Science Research Institute, People's Republic of China Liaoning Entry-out of Heilongjiang
Habitat Inspection and Quarantine Bureau, Shenzhen University.
The main drafters of this section. Juan Cao occasion, Ma Lidan, the soldiers, Cheng Chiu, Luan Feng Xia, Chen Ying, Liu Zhigang, Ethics, Wuhai Jiang.
Export of food allergens detection
Part 13. real-time PCR method
Detection wheat ingredient
1 Scope
This section provides the real-time PCR and fluorescence detection of food allergens wheat ingredients.
This section applies to food and feed ingredients wheat allergens qualitative detection.
This part of the minimum detection limit (LOD) of 0.01% (mass fraction).
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 27403 laboratory quality control of food molecular biology standardized testing
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Allergen alergen
Also known as allergens or allergens, can cause allergic reactions refers to an antigen.
3.2
Wheat wheat
Gramineae cultivation is an important grain wheat genus.
3.3
Real-time fluorescence PCR realtimePCR
Join fluorophore in the PCR reaction system, the use of real-time monitoring of the accumulation of the fluorescent signal of the PCR amplification process.
3.4
Ct values cyclethreshold
When the number of cycles of each reaction tube fluorescent signal reaches the set value of the field experienced.
4 Method summary
The samples were milled to extract DNA, DNA as a template, using wheat housekeeping gene (GAG56D and Wx012) specific detection
Primers and probes for real-time fluorescence PCR amplification, according to the Ct value, the existence of allergen wheat component Analyzing samples.
5 Testing and Materials
Unless otherwise specified, reagents were analytical grade or biochemical reagents. Test water should comply with GB/T 6682 in a water specifications. All test
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1961.13-2013_English be delivered?Answer: Upon your order, we will start to translate SN/T 1961.13-2013_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of SN/T 1961.13-2013_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 1961.13-2013_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|