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Determination of antibiotics residues in animal derived food. MIA method
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SN/T 1750-2006
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Basic data | Standard ID | SN/T 1750-2006 (SN/T1750-2006) | | Description (Translated English) | Determination of antibiotics residues in animal derived food. MIA method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53;X22 | | Word Count Estimation | 22,266 | | Date of Issue | 2006-01-26 | | Date of Implementation | 2006-08-26 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry Standard Notice 2006 No. 4 (total of 76) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the foods of animal origin ��- lactams, tetracyclines, macrolides and aminoglycosides residue testing specimen preparation, preservation and biological suppression detection methods. This standard applies to meat, eggs, fish and shrimp in the detection of antibiotic residue screening, positive results need to use other methods for confirmation. | SN/T 1750-2006: Determination of antibiotics residues in animal derived food. MIA method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of antibiotics residues in animal derived food.MIA method
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Antibiotic residues in animal-derived foods
Detection method
Released on.2006-01-26
Implementation of.2006-08-26
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard refers to the.1996 edition of the "Food Hygiene Inspection Guidelines" of the Ministry of Health and Welfare of Japan, Chapter 6 "Animal Pharmaceuticals, Feeding
Additives".
Appendix A of this standard is a normative appendix, and Appendix B is an informative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Tianjin Entry and Exit Inspection of the People's Republic of China
Epidemic situation.
The main drafters of this standard. Huang Xiaorong, Li Shouyi, Zheng Jing, Yang Fang, Chen Bin, Luo Maohuang.
This standard is the first industry standard for entry-exit inspection and quarantine.
Antibiotic residues in animal-derived foods
Detection method
1 Scope
This standard specifies the test for the detection of β-lactams, tetracyclines, macrolides and aminoglycoside residues in animal-derived foods.
Sample preparation, preservation and microbial inhibition assay methods.
This standard applies to the screening of antibiotic residues in meat, eggs, fish and shrimp. Positive results must be confirmed by other methods.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
3 sample preparation and preservation
3.1 Sample preparation
Meat, fish and shrimp. Take some representative samples from the original sample, put the edible part into a high-speed tissue masher, and mix thoroughly.
Evenly, the sample is not less than 500g by the quartering method. Load into the cleaning container and mark it.
Egg. From the original sample, randomly split half (not less than 500g), fresh eggs need to be shelled. Mix the egg yolk and protein in the mixer
Evenly, put it into a clean container as a sample and mark it.
Samples must be protected from contamination or changes in residue levels during sample preparation.
3.2 Sample storage
Meat, fish, shrimp and eggs. Samples are stored below -18 °C.
4 Determination method
4.1 Method summary
After the sample was extracted by buffer, incubated, and centrifuged, the supernatant was taken for measurement. Discriminating the use of antibiotics to inhibit sensitive bacteria
Whether the sample contains antibiotics.
4.2 Equipment and materials
4.2.1 Incubator. 30 ° C ± 1 ° C, horizontal compartment.
4.2.2 Centrifuge.
4.2.3 High speed tissue masher.
4.2.4 Constant temperature water bath.
4.2.5 Vortex Oscillator.
4.2.6 Microscope. 10 × ~ 100 ×.
4.2.7 Vernier caliper. measuring range 0mm ~.200mm, accuracy 0.02mm or suppression ring measuring instrument.
4.2.8 Sterilization plate. a glass with a diameter of 90 mm, a flat bottom or a disposable plastic sterilization plate.
4.2.9 Round filter paper. 10mm in diameter and 1.1mm thick. Japan ToyoRoshiKaisha,Ltd. Product, or equivalent.
4.2.10 Krebs bottle.
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