SN/T 1611: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1611-2013 | English | 479 |
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Serological detection methods of Southern bean mosaic virus
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SN/T 1611-2013
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| SN/T 1611-2005 | English | 279 |
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Southern bean mosaic virus serological detection methods
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SN/T 1611-2005
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PDF similar to SN/T 1611-2013
Standard similar to SN/T 1611-2013 GB 1352 NY/T 1933 GB/T 10460 SN/T 3576 Basic data | Standard ID | SN/T 1611-2013 (SN/T1611-2013) | | Description (Translated English) | Serological detection methods of Southern bean mosaic virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B23 | | Classification of International Standard | 67.060 | | Word Count Estimation | 12,130 | | Older Standard (superseded by this standard) | SN/T 1611-2005 | | Quoted Standard | SN/T 2122; SN/T 1840 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the plant quarantine in Southern bean mosaic virus detection method based DAS- ELISA serology, immune electron microscopy and immuno-capture and RT-PCR. This standard applies to detect the entry and exit legume seeds, seedlings and | SN/T 1611-2013: Serological detection methods of Southern bean mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Serological detection methods of Southern bean mosaic virus
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1611-2005
Southern bean mosaic virus serological methods
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1611-2005 "Southern bean mosaic virus serological detection methods."
This standard compared with SN/T 1611-2005, the main technical changes are as follows.
--- Increased immune electron microscopy method;
--- Increased immuno-capture RT-PCR validation method;
--- Added Appendix southern bean mosaic virus related information.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. to Cui, Yang Cuiyun, Hupei Long, Tao Tingdian, India Liping.
This standard replaces the standards previously issued as follows.
--- SN/T 1611-2005.
Southern bean mosaic virus serological methods
1 Scope
This standard specifies the plant quarantine in southern bean mosaic virus based on serological DAS-ELISA, immune electron microscopy and immuno-capture
RT-PCR and other testing methods.
This standard applies to detect the entry and exit legume seeds, seedlings and mass poisoning mediator in southern bean mosaic virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 2122 entry and exit quarantine of plants and plant products sampled
SN/T 1840 plant virus immune electron microscopy method
3 Southern bean mosaic virus Basic Information
Chinese name. Southern bean mosaic virus
Synonyms. bean mosaic virus No. 4 Beanmosaicvirus4; Southern bean mosaic virus 1 Southernbeanmosaicvirus1; dish
Southern bean mosaic virus Beansouthernmosaicvirus.
English name. Southernbeanmosaicvirus Abbreviation. SBMV
Taxonomic position. Southern bean mosaic virus genus Sobemovirus
Transmission. the virus may be spread by a round-robin type fashion leaf beetle. Bean seed transmission probability of 1% to 5%, cowpea seed-borne probability of 5%
40%. Mechanical inoculation experiments easy to spread.
Other relevant information of the virus in Appendix A.
Principle 4
SBMV spherical particles with a diameter of about 30nm for an effective immune source, can be prepared high titer antisera. Established on the basis of
The DAS-ELISA and immune electron microscopy methods. Single split virus genome, according to the conserved sequence specific primers were designed, the establishment of a Free
Phytophthora capture RT-PCR validation methods.
5 Equipment and Reagents
5.1 Equipment
PCR instrument, the Reader, washer, electronic scales (sense of volume 1/1000g), electrophoresis, electrophoresis tank, gel imaging system, transmission electron microscopy
Mirror, water bath, high-speed refrigerated centrifuge, small desktop centrifuge, -80 ℃ ultra-low temperature refrigerators, autoclaves, ice maker, microwave, whirlpool
Oscillator.
5.2 Reagents
Unless otherwise stated, all test reagents were analytical grade. Southern bean mosaic virus serological test kits, RNA reverse
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