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SN/T 3576-2013: Detection of genetically modified components. Method for soybean using PCR-DHPLC
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| SN/T 3576-2013 | English | 209 |
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Detection of genetically modified components. Method for soybean using PCR-DHPLC
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SN/T 3576-2013
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PDF similar to SN/T 3576-2013
Standard similar to SN/T 3576-2013 GB 1352 NY/T 1933 GB/T 10460 Basic data | Standard ID | SN/T 3576-2013 (SN/T3576-2013) | | Description (Translated English) | Detection of genetically modified components. Method for soybean using PCR-DHPLC | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B23 | | Classification of International Standard | 67.060 | | Word Count Estimation | 8,889 | | Quoted Standard | GB/T 19495.7; GB/T 6682; SN/T 1193 | | Regulation (derived from) | AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the PCR-DHPLC detection of genetically modified soybean. This standard applies to genetically modified soybeans (roundup ready soybean, RRS) in the detection of genetically modified ingredients. | SN/T 3576-2013: Detection of genetically modified components. Method for soybean using PCR-DHPLC---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of genetically modified components. Method for soybean using PCR-DHPLC
People's Republic of China Entry-Exit Inspection and Quarantine Standards
GMO detection
Soybean PCR-DHPLC Detection
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, China Inspection
Quarantine Science Research Institute, Shenzhen Bo Rui Xiang Hui Biotechnology Co., Ltd., National Center for Nanoscience, Liaoning Academy of Agricultural Sciences.
The main drafters. Zhang Guiming, water Zhu Fang, only to Yu, Cheng Yinghui, apricot Ling, Wang Zhaohui, PLAIN, Huang Xin, Ouyang Hui, Wang Ying,
Zhong Jianzhong, Miao Jian Kun.
GMO detection
Soybean PCR-DHPLC Detection
1 Scope
This standard specifies the PCR-DHPLC detection of genetically modified soybean.
This standard applies to genetically modified soybeans (roundupreadysoybean, RRS) in the detection of genetically modified ingredients.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 19495.7 GMO detection methods of sampling and sample preparation
SN/T 1193 genetic testing laboratory technical requirements
Principle 3
Denaturing high-performance liquid chromatography (denaturinghigh-performanceliquidchromatography, DHPLC) is a simple
Single, fast, non-gel method for nucleic acid analysis, samples were analyzed at 50 ℃ conditions, the number of base pairs in the sample determines the order of elution peak, when over
Column acetonitrile concentration increases, the nucleic acid fragment will be from small to large order was eluted according to the relative molecular mass. This standard uses the multiplex PCR
The method for genetically modified soybeans containing genetically modified simultaneously amplified PCR products were analyzed by DHPLC, obtained by DHPLC
Elution peak marker to determine the relative molecular mass size comparison, it is determined whether or not having a heterologous gene composition and whether the genetically modified soybeans.
4 equipment, utensils and reagents
4.1 Equipment and Utensils
Tissue grinder, PCR instrument, DHPLC meter, water bath, electronic balance (precision. 1/1000 above), ordinary centrifuge, freezing high
Speed centrifuges, refrigerators, cold refrigerator, ice maker, oven temperature, pH meter, pipettes (0.1μL, 0.5μL, 2μL, 10μL, 20μL,
100μL, 200μL, 1000μL), UV-visible spectrophotometer, water machines, autoclaves, PCR tubes (0.2mL), centrifuge tubes (1.5mL,
2.0mL), Tip head (0.1μL ~ 10μL, 5μL ~ 200μL, 100μL ~ 500μL).
4.2 Reagents
CTAB extract, Tris saturated phenol, chloroform, isoamyl alcohol, isopropyl alcohol, 70% ethanol, RNA enzyme (10mg/mL), multiplex PCR
The reaction buffer solution (MultiplexPCRMix), TaqDNA polymerase, DHPLC Buffer A. TEAA (0.1mol/L, pH7.0), B
Nitrile (0.025%), DHPLC Buffer B. TEAA (0.1mol/L, pH7.0), acetonitrile (25%), DHPLC buffer D. acetonitrile
(75%, pH7.0), molecular weight marker (marker).
5 Sampling and sample preparation
According to GB/T 19495.7 performed.
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