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SN/T 1558-2017 English PDF

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SN/T 1558-2017: Quarantine protocol for avain encephalomyelitis
Status: Valid

SN/T 1558: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 1558-2017English329 Add to Cart 3 days [Need to translate] Quarantine protocol for avain encephalomyelitis Valid SN/T 1558-2017
SN/T 1558-2005English279 Add to Cart 3 days [Need to translate] Protocol of agar gel immunodiffusion test for avian encephalomyelitis Obsolete SN/T 1558-2005

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Basic data

Standard ID SN/T 1558-2017 (SN/T1558-2017)
Description (Translated English) Quarantine protocol for avain encephalomyelitis
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 14,163
Date of Issue 2017-05-12
Date of Implementation 2017-12-01
Older Standard (superseded by this standard) SN/T 1558-2005
Quoted Standard SN/T 2123
Regulation (derived from) State-Quality-Inspection-Accreditation (2017) 228
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the virus isolation and identification of avian encephalomyelitis, reverse transcription-polymerase chain reaction, indirect immunofluorescence assay, enzyme-linked immunosorbent assay, agar diffusion assay. This standard applies to the quarantine of avian encephalomyelitis.

SN/T 1558-2017: Quarantine protocol for avain encephalomyelitis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for avain encephalomyelitis People's Republic of China entry and exit inspection and quarantine industry standards Replace SN/T 1558-2005 Avian encephalomyelitis quarantine technical specification Released on.2017-05-12 2017-12-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued Entry-Exit Inspection and Quarantine Industry Standard Avian encephalomyelitis quarantine technical specification China Standard Press Publishing First edition, April.2018

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces SN/T 1558-2005 "Avian Encephalomyelitis Agar Immunodiffusion Test Operating Procedures". Compared with SN/T 1558-2005, the main technical changes of this standard are as follows. --- Increased the isolation of avian encephalomyelitis virus; --- Increased reverse transcription-polymerase chain reaction; --- Increased indirect immunofluorescence assay; --- Increased enzyme-linked immunosorbent assay. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine. The main drafters of this standard. Yang Junxing, Sun Jie, Liao Lishan, Zhang Caihong, Liu Jianli, Tao Hong, Lin Yanxing, Lv Jianqiang, Cao Yufu, Lu Tiekang, Hua Qunyi, Qin Zhifeng. The previous versions of the standards replaced by this standard are. ---SN/T 1558-2005. Avian encephalomyelitis quarantine technical specification

1 Scope

This standard specifies the isolation and identification of avian encephalomyelitis virus, reverse transcription-polymerase chain reaction, indirect immunofluorescence test, enzyme-linked immunoassay Detection technology for epidemic adsorption test and agar diffusion test. This standard applies to the quarantine of avian encephalomyelitis.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 2123 Specification for the collection, transportation and preservation of animal quarantine samples for entry and exit

3 Abbreviations

The following abbreviations apply to this document. AE. Avian encephalomyelitis (see Appendix A) (Avianencephalomyelitis) AEV. Avian encephalomyelitis virus AGID. Agar diffusion test (Agargelimmunodiffusiontest) BSA. Bovine serum albumin (Bovineserumalbumin) CEF. Chicken embryo fibroblasts (Chickenembryofibroblast) ELISA. Enzyme-linked immunosorbent assay (Enzyme-linked immunosorbent assay) PBS. Phosphate buffer (Phosphatebuffersaline) RT-PCR. Reverse Transcription-Polymerase Chain Reaction

4 Virus isolation and identification

4.1 Equipment, equipment and reagents 4.1.1 Main equipment and equipment. autoclave, refrigerator, biological safety cabinet, centrifuge, sterile scissors, tweezers, plates, beakers, glass bottles, Tissue grinder, syringe, etc. 4.1.2 Materials. SPF eggs, SPF chicks. 4.1.3 Main reagents. normal saline, penicillin (10000 IU/mL), streptomycin (10000 μg/mL). 4.2 Sample collection, storage and transportation After the brain tissue of the chicks is aseptically taken, it should be treated immediately (see 4.3). If it cannot be treated temporarily, it can be stored in a -70 °C refrigerator. Sample protection Storage and shipping are performed in accordance with SN/T 2123. 4.3 Sample processing The tissue sample was aseptically weighed, ground, repeatedly frozen and thawed 3 times, and diluted with a sterile physiological saline solution for 1.5 (W/V) to prepare a suspension.

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