SN/T 1555: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1555-2020 | English | RFQ |
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(Technical specification for quarantine of chicken infectious laryngotracheitis)
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SN/T 1555-2020
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| SN/T 1555-2005 | English | 279 |
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3 days [Need to translate]
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Protocol of ager gel immunodiffusion test for avian infectious laryngotacheitis
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SN/T 1555-2005
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PDF similar to SN/T 1555-2020
Basic data | Standard ID | SN/T 1555-2020 (SN/T1555-2020) | | Description (Translated English) | (Technical specification for quarantine of chicken infectious laryngotracheitis) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Date of Issue | 2020-12-30 | | Date of Implementation | 2021-07-01 | | Older Standard (superseded by this standard) | SN/T 1555-2005 | | Regulation (derived from) | General Administration of Customs Announcement No. 136 [2020] | | Issuing agency(ies) | General Administration of Customs | SN/T 1555-2005: Protocol of ager gel immunodiffusion test for avian infectious laryngotacheitis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of ager gel immunodiffusion test for avian infectious laryngotacheitis
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Chicken infectious laryngotracheitis agar immunodistribution
Test procedure
Released on.2005-02-17
2005-07-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
Drafters of this standard. Xue Huaping, Liu Bo, Cheng Si.
This standard is the first industry standard for entry-exit inspection and quarantine.
Chicken infectious laryngotracheitis agar immunodistribution
Test procedure
1 Scope
This standard specifies the method of operation for avian agglutination of the infectious laryngotracheitis.
This standard applies to the detection of antibodies to infectious laryngotracheitis in chickens.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods (eqv ISO 3696.1987)
3 equipment and reagents
3.1 Equipment
3.1.1 Plate. The diameter of the plate is 90mm.
3.1.2 Micro sample applicator.
3.1.3 Plastic tips.
3.1.4 Puncher. Inner diameter 4.0mm, hole spacing 3.0mm, 7-hole plum-like metal puncher.
3.2 Reagents
3.2.1 Chicken infectious laryngotracheitis agar immunodiffusion test antigen and standard positive serum.
3.2.2 Agarose.
3.3 Solution preparation
The preparation method of pH7.2 0.01mol/L phosphate buffer solution and 1% thimerosal solution is shown in Appendix A.
GB/T 6682 requirements.
3.4 Tested serum
Under sterile conditions, venous blood is collected for about 1 mL. The blood sample is separated by serum and stored at 4 ° C for examination. If long-term storage is required, it is placed.
-20 ° C refrigerator.
4 steps
4.1 Agar plate preparation
1.0 g of agarose was weighed, and pH 7.2 0.01 mol/L phosphate buffer solution was added to 100 mL.
After heating and thawing, 8.0 g of sodium chloride was added, and after sufficiently dissolved, 1.0 mL of a 1% thimerosal solution (final concentration. 0.01%) was added.
Cool to 45 ° C ~ 50 ° C, clean and dry, 90mm diameter sterilization plate, placed on a horizontal platform, each plate plus
18 mL, thickness is about 3.0 mm.
After solidification, the lid is placed tightly, and the dish is placed upside down in a refrigerator at 4 ° C for storage.
4.2 punching
The reaction well is now used. Remove the agar plate from the refrigerator and punch it with a 7-hole set of plum-shaped punchers (1 hole in the middle, outer circumference)
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