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SN/T 1195-2003: Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in soybeans
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1195-2003 | English | 259 |
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Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in soybeans
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SN/T 1195-2003
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PDF similar to SN/T 1195-2003
Standard similar to SN/T 1195-2003 GB 1352 NY/T 1933 GB/T 10460 SN/T 3576 Basic data | Standard ID | SN/T 1195-2003 (SN/T1195-2003) | | Description (Translated English) | Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in soybeans | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B23 | | Word Count Estimation | 8,856 | | Date of Issue | 2003-03-17 | | Date of Implementation | 2003-09-01 | | Quoted Standard | GB/T 6682; SN/T 1193; SN/T 1194; SN/T 1204 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the genetically modified soybean qualitative polymerase chain reaction (PCR) detection methods. This standard applies to glyphosate-resistant soybeans genetically modified soybeans (roundup ready soybean) in GMO detection. | SN/T 1195-2003: Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in soybeans
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of the qualitative polymerase chain reaction (PCR) for detecting genetically modified component in soybeans
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Qualitative PCR of genetically modified components in soybean
Detection method
Released on.2003-03-17
2003-09-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Jiangsu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Jiang Yuan, Zhu Changqing, Lin Hong.
This standard is the industry standard for inspection and quarantine issued for the first time.
Qualitative PCR of genetically modified components in soybean
Detection method
1 Scope
This standard specifies the qualitative polymerase chain reaction (PCR) detection method for transgenic components in soybean.
This standard applies to the detection of transgenic components in soybean glyphosate-tolerant transgenic soybeans (roundupreadysoybean).
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
SN/T 1193 Genetic Testing Laboratory Technical Requirements
SN/T 1194 Sampling and sample preparation methods for detection of genetically modified components of plants and their products
Qualitative test method for real-time fluorescent PCR of genetically modified components in SN/T 1204 plants and their processed products
3 Terms, definitions and abbreviations
The following terms, definitions and abbreviations apply to this standard.
3.1
A functional gene sequence that does not have the species itself but is derived from other species.
3.2
The template gene sequence is first denatured to a single strand by high temperature, and is set according to the template sequence under the action of DNA polymerase and suitable reaction conditions.
The two primers are respectively annealed to the corresponding complementary sequence on the two strands of the template DNA, and then combined with each other, followed by DNA polymerization.
Under the action of the enzyme, four deoxyribonucleic acids (dNTPs) are used as substrates to allow the primers to be extended, and then the denaturation, annealing and extension are repeated.
In one cycle, the gene fragment to be amplified is amplified in geometric multiples.
3.3 Abbreviations
3.3.1 Lectin. A plant lectin gene that is an endogenous gene contained in soybean itself.
3.3.2 CaMV35S. 35Spromoterfromcauliflowermosaicvirus, cauliflower mosaic virus 35S promoter.
The nopaline synthase gene terminator.
3.3.4 CP4EPSPS. 5-enolpyruvylshikimate-3-phosphatesynthasegene, 5-enolpyruvylshikimate-3-phosphate
Synthetase gene.
3.3.5 RRS. roundupreadysoybean, a genetically modified herbicide for glyphosate approved by Monsanto, USA
Due to soybean, the foreign gene transferred to the CaMV35S promoter, NOS terminator and CP4-EPSPS gene.
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