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SN/T 1147-2019

Chinese Standard: 'SN/T 1147-2019'
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SN/T 1147-2019EnglishRFQ ASK Days<=3 (Method for quarantine identification of coconut leaf) Valid SN/T 1147-2019
SN/T 1147-2019ChineseRFQ ASK <=1-day [PDF from Chinese Authority, or Standard Committee, or Publishing House]  


Standard ID SN/T 1147-2019 (SN/T1147-2019)
Description (Translated English) (Method for quarantine identification of coconut leaf)
Sector / Industry Commodity Inspection Standard (Recommended)
Date of Issue 2019-09-03
Date of Implementation 2020-03-01
Older Standard (superseded by this standard) SN/T 1147-2002
Regulation (derived from) Natural Resources Department Announcement No. 7 of 2019

SN/T 1147-2019
(Method for quarantine identification of coconut leaf)
ICS 65.020B16
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
SN/T 1147-2019 replaces Sn/T 1147-2002
Cocoa leaf quarantine identification method
2020-03-01 implementation
Published by the General Administration of Customs of the People's Republic of China
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This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces SN/T 1147-2002 "Plant Quarantine Coconut Leaf Leaf Quarantine Identification Method", compared with SN/T 1147-2002,
The main technical changes are as follows.
--- Added approximate species differences and related search tables;
--- Added morphological feature map;
--- Added other relevant information about the biological characteristics, distribution and hazards of Cocoa leaf beetle.
This standard is proposed and managed by the General Administration of Customs of the People's Republic of China.
Responsible for drafting this standard. Shenzhen Academy of Inspection and Quarantine, Shenzhen Customs of the People's Republic of China, Arch of the People's Republic of China
North Customs.
The main drafters of this standard. Zhang Weifeng, Lin Wei, Yu Daojian, Xu Lang, Lu Xiaoyu, Zheng Yun.
The previous versions of the standards represented by this standard are.
--- SN/T 1147-2002.
SN/T 1144-2019
Cocoa leaf quarantine identification method
1 Scope
This standard specifies the methods of quarantine and identification of Coccinellidae in plant quarantine.
This standard applies to the quarantine and identification of coconut palm leaf beetles carried in plants and their products.
2 Normative references
The following documents are essential for the application of this document. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
SN/T 2122 Sampling Methods for Entry and Exit Plants and Plant Products Quarantine
3 Terms and definitions
The following terms and definitions apply to this document.
It means that the two claws are flat, and the angle formed by the base of the two is very large, generally more than 120 ° or even close to 180 °.
4 Coconut Leaf Methyl
Taxonomic status. Coleoptera, Chrysomelidea, Hispididae, Aniso-
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Coconut leaf beetle spreads with the host plant over long distances, and can also spread by short distances on its own.
See Appendix A and Appendix B for additional information on Coconut leaf beetle.
5 Method principle
Based on the biological characteristics of Coccinellidae and the habit of adult colonies, the morphological characteristics of adults are used as the main reference
Fixed basis.
6 Equipment and reagents
6.1 Equipment
Magnifying glass, stereo microscope, microscope, dissecting needle, tweezers, small writing brush, finger tube, label, scissors, white porcelain plate, insect box (specifications are
24cm × 16cm × 9cm).
6.2 Reagents
75% ethanol, glycerol, preservation solution (75% ethanol. glycerol = 101), distilled water.
7 On-site quarantine
Random check the host plants at the quarantine site, see SN/T 2122.
8 laboratory testing
8.1 Sampling
Use a magnifying glass to carefully search the entry and exit palm plant seedlings or plants. According to the biological characteristics of Coccinellidae, first observe the periphery of the plant.
If the old leaves have brown barred "burns" caused by feeding on the coconut leaf beetles, if it is suspicious, the unexpanded heart leaves should be checked carefully, and the hands should be tightly closed
Press the heart leaf, open the joint seam, and poke the happy leaf in the homeopathy. Check whether it is harmful for feeding. Adults, larvae and pupae often hide in the unexpanded heart leaf.
In the film, adult worms have the habit of infestation, and many pairs of cocoon leaf beetle may be found on one leaf to damage and feed; eggs often adhere to adult and
The larval-fed leaf-feeding mark feces are mostly scattered in single, and there are also multiple (two to six) connections arranged longitudinally along the leaf veins; according to
The worm's harmfulness focuses on strengthening the quarantine of palm plants that are three to four years old. At the same time, we must also pay attention to the container and outer packaging carton.
Wait for the loading container to carry out the inspection. Collect the various pest-like pests found by inspection with tweezers or a writing brush and store them in a finger tube (75% of larvae can also be used).
Alcohol preservation solution), labeled, brought back to the laboratory for breeding and identification.
8.2 Larval rearing
Larvae of 1st to 3rd instars and 4th to 5th instars are reared separately. When replacing the host leaves, pick the larva with a writing brush and reduce the number of hands
Contact, and timely pick out the dead insects with mold. Host blades are changed every 2 to 3 days. Feeding room temperature 25 ℃ ± 1 ℃, relative
The humidity is 75% ± 5%, and the light is 12h.
8.3 Microscope observation
In the laboratory, the worm-like specimens of the coconut leaf beetle were identified by means of dissecting microscope observation.
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9 Identification characteristics
9.1 Identification characteristics
--- Mouth behind the head, the mouth is visible on the ventral surface.
--- The antennae are mostly 11 knots, generally filiform, and the two antennae are very close to each other and sometimes almost connected.
--- The shape of the pronotum is square, semi-circular, etc. There are branches on the sides and back.
9.2 Identification of subfamilies
--- The body is generally long, without spines on the back, and the elytra sometimes has a spine.
--- The head is inserted into the chest cavity shallowly, all the mouth organs are exposed, the edge of the mouth is almost round, and the length and width are equal or the width is greater than the length.
--- The front lip base is obvious, and the hind leg segments extend beyond the side edges of the coleoptera.
--- Fully open claws without egg sheath.
--- Larvae are parasitic, with ossifying discs at the end of the abdomen.
--- The head has interphasic protrusions and the ends are not curved, and the length is more than half of the antennae stem length.
--- The front side of the pronotum is round and slightly extended, the back side angle is simple or has 1 small tooth to 2 small teeth or sharp corners, and the back has engraved points.
--- Elytra has a short carved shield line.
9.4 Morphological Characteristics of Coconut Leaf Turtle
9.4.1 Adult
The body is slender and flat, with a length of 7mm to 10mm, and the width of the elytra is about 2mm.
It has villi, and the stalk section is 2 times as long as the head. The head is significantly narrower than the pronotum, and there is an angular process in front of the top of the head.
One-half, tapered from the base to the end, not truncated; there is a light brown longitudinal groove rearward along the corner; the middle part of the head is too wide;
Color, length equal to width, with thick and irregular cut points more than 100 grains; coleoptera blue-black, a few individual coleoptera base about one-quarter to one-half
It is red-yellow, and the engraved points are arranged vertically. The size of the engraved points is narrower than the horizontal distance of the engraved points. The engraved point area is flat except for the two sides and the ends; the feet are yellow, short, thick and
Slightly flat (see Figure C.1a in Appendix C).
9.4.2 Egg
Oval, brown, flat, 1.5mm long and 1.0mm wide; microscopic examination can reveal honeycomb flat protrusions on the epidermis of the egg (see attached
Figure C in Record C. 1b) The lower epidermis adhered to the host blade is smooth without protrusions.
9.4.3 Larva
The mature larvae are flat, milky white to pale yellow; the head is swollen and rounded on both sides; there is a pair of spike-like lateral processes on both sides of the prothorax and each ventral segment, and the abdomen is 9
Knots, 8th and 9th merge, forming a pair of inwardly curved caudate processes at the end, the outer sides of the two processes are almost parallel at the base, and the processes are gradually tapered
(See Figure C.1c, Figure C.1d, and Figure C.1f in Appendix C).
9.4.4 蛹
The morphology is similar to the larva, but the body color is darker, the individual is thicker, with winged buds and feet, and a pair of caliper-shaped protrusions remain at the end of the belly, but the valve at the base
The opening disappeared (see Figure C.1f in Appendix C for morphology).
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10 result judgment
Based on the morphological characteristics of adults, the characteristics were identified step by step based on the characteristics of 9.1 to 9.4. The morphological characteristics that conform to the description of 9.4.1 can be identified as coconut
11 specimen preservation
The collected specimens should be properly stored. Pest specimens can be stored in 75% ethanol-containing specimen bottles or made into pin-inserted specimens. Harm
Specimens are preserved after deworming, and the preservation period is not less than 6 months.
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Appendix A
(Informative appendix)
Additional Information
A. 1 Distribution
Domestic distribution is mainly in Guangzhou, Guangdong, Shenzhen, Zhuhai, Foshan, Jiangmen, Dongguan, Zhongshan, Zhanjiang, Maoming, Guangdong,
Shantou, Jieyang, Shanwei, Yangjiang, Qingyuan; Beihai, Guangxi Zhuang Autonomous Region; Haikou, Sanya, Wenchang, Qionghai, Hainan
City, Wanning City, Wuzhishan City, Quzhou City, Dongfang City, Ding'an County, Tunchang County, Chengmai County, Lingao County, Lingshui Li Autonomous County, Baoting Li Miao
Autonomous County, Ledong Li Autonomous County, Changjiang Li Autonomous County; Hong Kong Special Administrative Region; Taiwan Province.
Overseas distribution mainly in Indonesia, Malaysia, Vietnam, Maldives, Australia, Nauru, Fiji, Papua New Guinea,
Nuato, Solomon Islands, New Caledonia, New Hebrides, Western Samoa, Micronesia, French Polynesia, Weas
Mai Islands, Guam (US)
A. 2 Hosts and hazards
A. 3 Biological characteristics
Coconut leaves have a single diet, and mainly damage the heart leaves of palm plants to larvae and adults. Females drill into heart leaves to lay eggs and emerge for about 3 weeks
Then spawning begins, and each female can lay more than 100 eggs. The egg stage is about 5 days; the larva is 3 to 6 years old, and the developmental period is about 30 to 40 days; the pupal stage
Five to six days; the adult life span is from March to June; the average generation cycle is about 120 days, with three to six generations per year, and generations overlap significantly. Adult
The average life span is 156 days, the longest is 235 days, and the male-female ratio is 1. 1. Adults can mate after emergence, and females and males can mate multiple times in their lifetime. to make
Insects gathered at the base of the unfolded heart leaf. Adults have a certain flying ability and pseudo-death phenomenon, and can spread by flying at short distances. Long distance
Dissemination by means of host transfer.
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Appendix B
(Informative appendix)
Retrieval form of Coccinellidae and its approximate species
Antenna non-serrated 2
Not all 4 corners of the pronotum form 2 teeth 3
3 The front side of the pronotum is not prominent. 4
The front side of the pronotum is prominently protruding 5
Male individual head interphasic process not exceeding antennae Section 2 6
6 head plates longer than 7
Overhead plate length is less than width 9
The front end of the head plate expands, and the male intercranial protrusion is equal to the antennal stalk section 8
Nine-angled process is generally less than half of the antennal stalk
Intercorneal processes are generally larger than half of the antennal stalk
Chest length and width are similar; body is slender 12
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Appendix C
(Informative appendix)
Morphological characteristics of Coccinellidae and its approximate species
a --- Coconut Leaf Beetle Adult
b --- Coconut Leaf Eggs
c --- 1st instar larva
d --- tail coccyx larvae
e --- Old mature larva
f --- coconut leaf
Figure C. 1 Coconut Leaf Turtle
Note. Figure C. 1a Imitates Gressit 1960, Figure C. 1b to Figure C. 1f imitation Maulik 1938.
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[1] J. LinsleyGressit. Papuan-westpointlines Hispanebeetes [J]. Paciffinces, 1962 (1).
1 to 90.
[2] Huang Fayu, Liang Qiongchao, Lai Tianzhong, et al. Coconut leaf beetles and red-brown weevil were intercepted many times at the South China Sea port [J]. Phytosanitary,.2000, 14
(2). 69.
[3] Huang Fayu, Liang Guangqin, Liang Qiongchao, et al. Quarantine and control of Coccinellidae [J]. Plant Quarantine,.2000, 14 (3). 158 ~ 160.
[4] Lu Yongyue, Zeng Ling. Analysis of the introduction pathways and invasion causes of Coccinellidae [J]. Chinese Forest Pests,.2004, 23 (4). 12-15.
[5] Zhou Rong, Zeng Ling, Liang Guangwen, et al. Observation on the biological characteristics of Coccinellidae experimental population [J]. Insect knowledge..2004, 41 (4).
336 to 339.
[6] Zhou Rong, Zeng Ling, Cui Zhixin, et al. Observation on the morphological characteristics of Coconut Leaf Turtle [J]. Phytosanitary..2004, 18 (2). 84 ~ 85.
[7] Zeng Ling, Zhou Rong, Cui Zhixin, et al. Effects of host plants on the growth and development of Coccinellidae [J]. Journal of South China Agricultural University
Academic Edition),.2003, 24 (4). 37 ~ 39.
[8] Zhong Yihai, Liu Kui, Peng Zhengqiang, et al. Coconut leaf beetle-a new high-hazard insect [J]. Tropical Agricultural Sciences,.2003, 23 (4).
67 ~ 71.
[9] Zhou Huanqi, Ma Zilong, Qin Weiquan, etc. Parasitic natural enemies of Coconut Leaf Beetles
Indoor cultivation [J]. Chinese Journal of Biological Control..2004, 22 (Supplementary). 6-10.
[10] Animal and Plant Quarantine Bureau of the People's Republic of China, Plant Quarantine Laboratory of the Ministry of Agriculture. Selection of China's Entry Plant Quarantine Pests
[M]. Beijing. China Agricultural Press,.1997.
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Related standard:   SN/T 1148-2019  SN/T 1149-2019
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