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SN/T 0177-2011 English PDF

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SN/T 0177-2011: Method for enumeration of clostridium perfringens in food for export
Status: Valid

SN/T 0177: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 0177-2011English209 Add to Cart 3 days [Need to translate] Method for enumeration of clostridium perfringens in food for export Valid SN/T 0177-2011
SN 0177-1992English319 Add to Cart 3 days [Need to translate] Method for detection of clostridium perfringensin food for export Obsolete SN 0177-1992

PDF similar to SN/T 0177-2011


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Basic data

Standard ID SN/T 0177-2011 (SN/T0177-2011)
Description (Translated English) Method for enumeration of clostridium perfringens in food for export
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X09
Word Count Estimation 8,887
Date of Issue 2011-09-30
Date of Implementation 2012-04-01
Older Standard (superseded by this standard) SN 0177-1992
Quoted Standard GB/T 6682; SN/T 1538.1; SN/T 1538.2
Regulation (derived from) National-Quality-Inspection-accreditation (2011) 492
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the export of food in Clostridium perfringens (Clostridium perfriingens) count test method. This standard applies to the export of food in Clostridium perfringens tests.

SN/T 0177-2011: Method for enumeration of clostridium perfringens in food for export

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for enumeration of clostridium perfringens in food for export People's Republic of China Entry-Exit Inspection and Quarantine Standards Replacing SN 0177-1992 Food exports Clostridium perfringens Bacillus counting method Issued on. 2011-09-09 2012-04-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard replaces SN 0177-1992 "food for export Clostridium perfringens test methods." This standard compared with SN 0177-1992, the main technical changes are as follows. --- Change to the recommended standards; --- Detection section reference ISO 7937.2004 "food and animal feed microorganism Clostridium perfringens count Count the number of colonies technology "content, increasing the lactose in the standard - sulfite tested as a confirmatory test, subtraction containing yolk TSC agar and PY media use; --- Supplementary commercialization of automated microbial identification system. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Gu Ming, Han Wei, Xie Jue, Wang win, Chen. Food exports Clostridium perfringens Bacillus counting method

1 Scope

This standard specifies the export of food products Clostridium perfringens (Clostridiumperfringens) count test. This standard applies to the export of food in Clostridium perfringens test.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium SN/T 1538.2 media preparation Guide Part 2. A Practical Guide to medium performance test

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Clostridium perfringens clostridiumperfringens On a specific selective medium, bacterial colony growth characteristics appears black (sulfite degradation sulfides black precipitate formed Thereof, and the colony is black), break down lactose produce acid and gas, 48h within bacteria can liquefy gelatin.

4 Equipment and Materials

4.1 sterile pipette. 1.0mL and 10.0mL, sub-scale were 0.1mL and 1.0mL. 4.2 sterile Petri dish. diameter of 90mm. 4.3 homogenizer and homogenized bags (homogeneous or cup). 4.4 incubator or anaerobic incubator. 37 ℃ ± 0.5 ℃. 4.5 temperature water bath. 46 ℃ ± 0.5 ℃. 4.6 Anaerobic generating means. 4.7 magnifier and (or) colony counter. 4.8 optical microscope 10 × ~ 100 ×. 4.9 Refrigerator. 2 ℃ ~ 8 ℃. 4.10 balance. a sense of the amount of 0.1g. 4.11 automated microbial identification system VITEKcompact1). 1) Product name supplied by the French company bioMérieux products. This information is given for the convenience of users of this standard does not mean that the product Recognition. If other equivalent product has the same effect, you can use these equivalent products.

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