Search result: SN/T 0173-2018 (SN/T 0173-2010 Older version)
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 0173-2018 | English | RFQ |
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(Vibrio parahaemolyticus in export foods)
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SN/T 0173-2018
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| SN/T 0173-2010 | English | 799 |
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Determination of Vibrio Parahaemolyticus in food for import and export
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SN/T 0173-2010
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| SN/T 0173-1992 | English | RFQ |
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Method for detection of VibrioParahaemolyticus in food for export
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SN/T 0173-1992
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| Standard ID | SN/T 0173-2018 (SN/T0173-2018) | | Description (Translated English) | (Vibrio parahaemolyticus in export foods) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Date of Issue | 2018-03-12 | | Date of Implementation | 2018-10-01 | | Older Standard (superseded by this standard) | SN/T 0173-2010 | | Regulation (derived from) | National Quality Inspection (2018) 76 |
SN/T 0173-2010
Determination of Vibrio Parahaemolyticus in food for import and export
Exit inspection and quarantine industry standard book People's Republic of China
Replacing SN 0173-1992
Importers and Vibrio parahaemolyticus in food testing methods
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard replaces SN 0173-1992 "export food inspection method of Vibrio parahaemolyticus."
The standard reference ISO /T S21872-1.2007 "Microbiology of food and feed testing methods Vibrio spp level test
Part 1. Vibrio parahaemolyticus and Vibrio cholerae test (Microbiologyoffoodandanimalfeedingstuffs-
(FDABacteriologicalAnalyticalManualChapter9Vibrio, 2004) of the original standard text format, some of the wording and
Text be revised, but the main test methods and technical route without making changes.
This standard compared with SN 0173-1992, the main changes are as follows.
--- The original standard name revised to "import and export food in Vibrio parahaemolyticus test methods";
--- The range has been revised;
--- Increased feed methods and processes;
--- Increased shelled shellfish preparation and dilution of the sample in the detection step;
--- The Vibrio parahaemolyticus qualitative and quantitative detection steps were described;
--- Increased chromogenic culture medium screening tests;
--- The revised report results as "qualitative results Report" and "the most approximation (MPN) Results Report";
--- Will be part of the revised formulation media, reagents reference ISO /T S21872-1 methods.
This standard Annex A, Annex D is normative appendix, Appendix B, Appendix C is informative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Entry-Exit Inspection and Quarantine Tianjin, Shandong Entry-Exit Inspection
Inspection and quarantine bureau.
The main drafters of this standard. Xu Junyi, Juan Cao occasion, Gao Qi Li, Cheng Chiu, Ray quality text, Wangqiu Yan, Jiang Dan, Ma Hui Rui, Song Huijun,
Lu Hangan.
This standard replaces the standards previously issued as follows.
--- SN 0173-1992.
Importers and Vibrio parahaemolyticus in food testing methods
1 Scope
This standard specifies the imported and exported food Vibrio parahaemolyticus qualitative detection methods and the best approximation (MPN) counting method.
This standard applies to foods Vibrio parahaemolyticus inspection, animal feed and other food production and processing sub-regional environmental samples
Vibrio parahaemolyticus test can refer to use.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB 19489 General requirements for laboratory biosafety
SN 0330 export food microbiological examination General
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1538.2 media preparation Guide Part 2. A Practical Guide to medium performance test
3 Sampling and sample preparation
3.1 Sampling
The number of samples and sampling methods carried out by the SN 0330.
3.2 Sample Preparation
3.2.1 Fish
Take the fish in different parts.
3.2.2 shelled shellfish
Should be washed in clean running water and housing, the housing disinfected with 70% ethanol, then aseptically cut the adductor muscle, open shells. take
All the shellfish meat and shellfish solution containing offal. Each test sample shall include at least 12 shellfish individuals.
3.2.3 crustaceans
Including the gills and intestines take part or whole.
3.3 samples were thawed and save
Frozen samples should be below 45 ℃ not more than 15min or 2 ℃ ~ 5 ℃ not more than 18h thaw. If not timely inspection, should be placed
At about -15 ℃ stored in the test within 48h; not frozen and perishable samples should be as timely inspection, if not timely inspection, should be placed
6 ℃ ~ 10 ℃ refrigerator for inspection within 24h.
4 Equipment and Materials
4.1 tube. 15mm × 150mm, 10mm × 100mm.
4.2 straw. 1mL, 10mL.
4.3 dish. diameter of 90mm.
4.4 homogenizer. 8000r/min ~ 10000r/min.
4.5 thermostat. 36 ℃ ± 1 ℃.
4.6 constant temperature water bath. 42 ℃ ± 1 ℃.
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