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SN/T 0169-2010: Determination of coliform, fecal coliform and Escherichia coli in food for import and export
Status: Valid SN/T 0169: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 0169-2010 | English | 449 |
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Determination of coliform, fecal coliform and Escherichia coli in food for import and export
| Valid |
SN/T 0169-2010
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| SN 0169-1992 | English | 439 |
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Method for examination of coliform, fecal coliformand escherichia coli in food for export
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SN 0169-1992
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PDF similar to SN/T 0169-2010
Basic data | Standard ID | SN/T 0169-2010 (SN/T0169-2010) | | Description (Translated English) | Determination of coliform, fecal coliform and Escherichia coli in food for import and export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X09 | | Word Count Estimation | 17,179 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Older Standard (superseded by this standard) | SN/T 0169-1992; SN 0333-1994; SN/T 1059.2-2002 | | Quoted Standard | SN/T 1538.1; SN/T 1538.2 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export food human intestinal flora, fecal coliforms and E. coli detection methods. This standard MPN method is suitable for import and export food colon Yin Qun, fecal coliforms and E. coli testing, plate count method for coliforms in food import and export inspection, ��-glucosidase assay for feed food exports (excluding shellfish) in E. coli testing, filter/MUG method is suitable for import and export of instant noodles, puffed foods, mineral water, soft drinks, milk (required protease treatment), crystal sugar and pepper grains coliform test group and E. coli. | SN/T 0169-2010: Determination of coliform, fecal coliform and Escherichia coli in food for import and export
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of coliform, fecal coliform and Escherichia coli in food for import and export
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Replacing SN 0169-1992, SN 0333-1994 and SN/T 1059.2-2002
Import and export food coliform, fecal coliform
And E. coli detection methods
Determinationofcoliform, fecalcoliformandEscherichiacoli
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard replaces SN 0169-1992 "export food coliform, fecal coliform and E. coli testing methods", SN 0333-
1994 "E. coli in food for export (glucosidase fluorescence) test methods" and SN/T 1059.2-2002 "import and export food coliform
Group, E. coli counts membrane/MUG law. "
This standard compared with SN 0169-1992, SN 0333-1994 and SN/T 1059.2-2002, the main technical changes are as follows.
--- The original standard name "food for export coliform, fecal coliform and E. coli test methods", "E. coli in food for export
Bacteria (glucosidase fluorescence) test methods "and" import and export food coliform and Escherichia coli counts membrane/MUG Law "whole
Co amended to "import and export food coliform, fecal coliform and E. coli detection methods";
--- Range SN 0169-1992 standards unchanged, but SN 0333-1994 and SN/T 1059.2-2002 standard scope
Limit, therefore, the scope of this standard will be revised to integrate;
--- Increasing the normative references;
--- Increasing the terms and definitions;
--- Deleted a mortar and pestle in equipment and materials, the dilution bottle to increase the "other suitable containers";
--- Liquid sample MPN method for the detection of amendments.
Liquid sample inoculum 1mL or more, with a double lauryl sulfate tryptone broth; and 1mL 1mL inoculum the following
Who is a single material lauryl sulfate tryptone broth;
--- Plate count method by deleting the formula;
--- MPN table value added "95% confidence interval."
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Qinhuangdao Exit Inspection and Quarantine, Inner Mongolia, People's Republic of China Entry-Exit Inspection and
Quarantine Bureau, Tianjin, People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Fubao Lian, Liu high school, Hou Liping, Goofy.
This standard replaces the standards previously issued as follows.
--- SN 0169-1992;
--- SN 0333-1994;
--- SN/T 1059.2-2002.
Import and export food coliform, fecal coliform
And E. coli detection methods
1 Scope
This standard specifies the import and export food coliform, fecal coliform and E. coli detection methods.
The standard method is suitable for import and export food MPN coliform, fecal coliform and E. coli tests; plate count method applicable
Coliform testing in the import and export food products; β- glucosidase fluorescence method is suitable for import and export food (excluding shellfish) in Escherichia coli
Inspection; filter/MUG law applicable to the import and export of instant noodles, puffed food, mineral water, soft drinks, milk (required protease treatment), and single-crystal sugar
Pepper grains coliforms and E. coli testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1538.2 media preparation Guide Part 2. A Practical Guide to medium performance test
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Coliform bacteria coliform
Aerobic and facultative anaerobic, can break down lactose at 37 ℃ 48h produce acid and gas group of gram-negative bacillus free.
3.2
Fecal coliform fecalcoliform
Aerobic and facultative anaerobic, capable of 44.5 ℃ ± 0.5 ℃, 24h ferment lactose produce acid and gas group of gram-negative bacillus without the
Heat-resistant coliform bacteria can be called (thermotolerantcoliformorganisms).
3.3
E. Escherichiacoli
Aerobic and facultative anaerobic, can be decomposed in 44.5 ℃ ± 0.5 ℃ 48h lactose produce acid and gas, chemical and biological characteristics "IMViC" to "- or
- - "No group of Gram-negative bacteria Bacillus can be called Escherichia coli.
4 test methods
4.1 Principle
4.1.1 The most likely approximation (mostprobablenumber, MPN) method
MPN method is a statistical combination of microbiology and a quantitative assay. After the test sample was serially diluted and cultured, not according to their
Minimum dilution growth and the growth of the highest dilution, using statistical probability theory deduced coliform, fecal coliform or E. coli
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