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LY/T 2428-2015 English PDF

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LY/T 2428-2015: Technical regulation for tissue culture of Cunninghamia lanceolate
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Basic data

Standard ID LY/T 2428-2015 (LY/T2428-2015)
Description (Translated English) Technical regulation for tissue culture of Cunninghamia lanceolate
Sector / Industry Forestry Industry Standard (Recommended)
Classification of Chinese Standard B61
Classification of International Standard 65.020.20
Word Count Estimation 13,165
Date of Issue 2015-01-27
Date of Implementation 2015-05-01
Quoted Standard GB/T 6001; LY/T 1000; LY/T 1882
Regulation (derived from) State Forestry Administration Announcement 2015 No. 6: Industry Standard Filing Announcement 2015 No. 2 (Total number 182)
Issuing agency(ies) State Forestry Administration
Summary This Standard specifies the fir seedling technology of tissue culture explants, medium, inoculation, tissue culture, transplanting and nursery management, investigation and nursery seedlings, technical files.

LY/T 2428-2015: Technical regulation for tissue culture of Cunninghamia lanceolate

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Technical regulation for tissue culture of Cunninghamia lanceolate ICS 65.020.20 B61 People's Republic of China forestry industry standards Chinese fir tissue culture nursery technical regulations Technical regulation fatissue culture of Cunninghamialanceolata 2015-01-27 released 2015-05-01 implementation State Forestry Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the Fujian Provincial Forestry Department. This standard by the National Forest Seeds Standardization Technical Committee (SAC/TC115) centralized. This standard was drafted. Fujian Provincial Academy of Forestry, Nanjing Forestry University, Fujian Yangkou state-owned forest farms, Fujian Province, State-owned music Forest farm. The main drafters of this standard. Zheng Renhua, Xiao Hui, Shi Jisen, Huang Jinhua, Fang Lu Ming, Li Yong, Xie Rugen, Ye Daiquan, Su Shunde, Fang Yanghui. Chinese fir tissue culture nursery technical regulations

1 Scope

This standard specifies the explants of fir tissue culture nursery technology, culture medium, inoculation, tissue culture, transplanting and nursery stock management, nursery stock Check and out nursery, technical files. This standard applies to fir tissue culture nursery production.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6001 nursery technical regulations LY/T 1000 container seedling technology LY/T 1882 tree tissue culture nursery technical regulations

3 explants

3.1 Selection of mother plants The donor parent of the explant should be the elite seed or the clonal ramets of the elite seed of Chinese fir. 3.2 explant collection 3.2.1 Acquisition time Should be good weather in spring or winter collection. 3.2.2 Acquisition sites and methods Mother plant neck or truncated truncated stem base of about 15cm long sprout, interception of semi-lignification, robust growth, no pests and diseases Buds as explants. 3.2.3 Deposit After the explants are collected, it is advisable to use moisturizing materials to wrap them and store them in a plastic foam container equipped with ice packs. The storage period should not exceed 2d ~ 3d. 3.3 surface sterilization 3.3.1 Cleaning Wash explants surface with neutral detergent solution, rinse with water for 30min-60min. 3.3.2 sterilization In the clean bench, soaking the explants with 75% alcohol 8s ~ 10s, rinsed with sterile water after pouring alcohol; then 0.1%

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