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LY/T 1926-2020: Test method for evaluating the antibacterial performance of wood(bamboo)-based panels and products
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| LY/T 1926-2020 | English | 169 |
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Test method for evaluating the antibacterial performance of wood(bamboo)-based panels and products
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LY/T 1926-2020
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| LY/T 1926-2010 | English | 399 |
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Antimicrobial wood (bamboo)-based flooring. Test method for antimicrobial function and antimicrobial effect
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LY/T 1926-2010
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Basic data | Standard ID | LY/T 1926-2020 (LY/T1926-2020) | | Description (Translated English) | Test method for evaluating the antibacterial performance of wood(bamboo)-based panels and products | | Sector / Industry | Forestry Industry Standard (Recommended) | | Classification of Chinese Standard | B69 | | Classification of International Standard | 79.080 | | Word Count Estimation | 7,769 | | Date of Issue | 2020-12-29 | | Date of Implementation | 2021-06-01 | | Older Standard (superseded by this standard) | LY/T 1926-2010 | | Regulation (derived from) | Announcement No. 25 (2020) of the State Forestry and Grassland Administration | | Issuing agency(ies) | State Forestry and Grassland Administration | LY/T 1926-2020: Test method for evaluating the antibacterial performance of wood(bamboo)-based panels and products
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Test method for evaluating the antibacterial performance of wood
(bamboo)-based panels and products
People's Republic of China Forestry Industry Standard
Replace LY/T 1926-2010
Wood-based panels and wood (bamboo) products
Antibacterial performance testing and classification
2020–12–29 released
2021–06–01 implementation
Issued by the State Forestry and Grassland Administration
Foreword
This document is in accordance with GB/T 1.1-2020 ``Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents''
The rules given are drafted.
This document replaces LY/T 1926-2010 "Antibacterial Wood (Bamboo) Floor Antibacterial Performance Test Method and Antibacterial Effect".
Compared with LY/T 1926-2010, in addition to editorial changes, the main technical content changes are as follows.
――The name of the document was revised to "Test and Classification of Antibacterial Performance of Artificial Panels and Wood (Bamboo) Products"
――1 Modified the scope of application;
――3 Terms and definitions, modify 3.1 antibacterial treatment, clarify the scope of antibacterial, and specifically refer to antibacterial function; add
3.2 Definition of antibacterial rate;
―In Clause 4.1, it is clear that the bacterial test method is the filming method;
――Added "4.2 Test Conditions", requiring that "bacteria testing laboratories should meet the requirements of GB 19489";
――Description of strain ATCC code. Code of American Type Culture Collection;
――4.8.1 The operation of the negative control sample (A) is to add sterile water without inoculating bacteria;
―The number of samples is changed from 5 to 3 (repeat once, there are 6 samples in total);
――4.10.1 The requirements for the role of negative controls have been added to the requirements for test results;
――Remove the test content related to mold;
――The content of "5 Antibacterial Performance Classification" has been added;
――Add 6 test report clauses.
This document was proposed and managed by the National Wood-based Panel Standardization Technical Committee (SAC/TC198).
Revision units of this document. Research Institute of Wood Industry, Chinese Academy of Forestry, National Quality Supervision of Wood-based Panels and Wood and Bamboo Products
Inspection Center, Zhejiang Agriculture and Forestry University, Dehua Rabbit Baby Decoration New Materials Co., Ltd., Baroque Wood (Zhongshan) Co., Ltd.
Company, Zhaoqing Modern Zhumei Home Furnishing Co., Ltd., Ruitong Polymer Technology (Zhejiang) Co., Ltd., Shengxiang Group Co., Ltd.
Company, Tianjin Shengshide New Material Technology Co., Ltd., Guangdong Tianyuan Huibang New Material Co., Ltd., Del Future Technology
Holding Group Co., Ltd., Scholar Mendi Group Co., Ltd., Guangzhou Chaopai Wood Industry Co., Ltd.
The main drafters of this document. Ma Xingxia, Lu Bin, Fu Yuejin, Sun Fangli, Zhang Bin, Zhang Xiaowei, Lin Deying, Zhong Yaocan,
Chunhua, Fan Wenming, Li Zhen, Dai Yanmei, Yao Hongpeng, Bu Lixin, Liang Faguo.
The previous editions of this document and the documents replaced are as follows.
──First released in.2010 as LY/T 1926-2010;
──This is the first revision.
Testing and grading of antibacterial properties of wood-based panels and wood (bamboo) products
1 Scope
This document specifies the antibacterial performance test methods and classification terms and definitions, antibacterial performance of wood-based panels and wood (bamboo) products
Detection method and classification.
This document is applicable to the antibacterial performance testing and grading of antibacterial wood-based panels and various antibacterial wood (bamboo) products. This document
Does not involve the safety evaluation of antibacterial products.
2 Normative references
The contents of the following documents constitute indispensable clauses of this document through normative references in the text. Among them, dated
For reference documents, only the dated version applies to this document; for undated reference documents, the latest version (including all
Some amendments) apply to this document.
Note. For undated references, if the latest version contains the quoted content, then the quoted
The final version of the content applies.
GB 4789.2-2016 National Food Safety Standard, Food Microbiological Inspection, Determination of Total Colony
GB 19489 Laboratory Biosafety General Requirements
WS/T 650-2019 Antibacterial and antibacterial effect evaluation method
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Antibacterial treatment
Use mechanical, physical or chemical methods to interfere with, inhibit the growth and reproduction of bacteria, reduce their number or kill directly
The process of bacteria.
3.2
Antibacterial rate
The difference between the average number of bacteria recovered after the test sample and the control sample are inoculated with the test bacteria for a certain period of time and the average recovery of the control sample
The percentage of the bacterial count is called the antibacterial rate of the test sample.
4 Detection method
4.1 Principle
The antibacterial test method refers to the filming method used in the WS/T 650-2019 standard, that is, by inoculating bacteria on the sample to be tested
The surface of the product is then covered with a plastic film, so that the bacteria evenly and fully contact the surface of the sample to be tested, and pass through under the specified conditions.
After culturing for a certain period of time to reach a certain number of bacteria, use the plate viable bacteria count method to calculate the number of bacteria in the tested sample.
A method to evaluate the antibacterial effect by calculating the antibacterial rate.
4.2 Test conditions
The bacteria testing laboratory shall meet the requirements of GB 19489.
4.3 Main instruments and equipment
-Constant temperature incubator (can keep the temperature at 37±2℃)
-Refrigerator (can keep the temperature at 0-5℃)
-Ultra-clean workbench (cleanliness level not less than 100)
-Biological optical microscope (200～400×)
-Pressure steam sterilizer (can maintain a temperature of 121°C and a pressure of 103kpa)
-Electric heating drying oven (can keep the temperature at 160-180℃)
-Inoculation loop (diameter not more than 4mm)
4.4 Cover film
Polyethylene film, size 40mm×40mm×0.05mm, soak in 75% ethanol solution for 1min, then use sterile water or
Rinse the eluate, dry it naturally and set aside.
4.5 Medium
4.5.1 Liquid LB medium
Yeast extract 5.0g
Tryptone 10.0g
Sodium chloride 5.0g
Preparation method. add the above ingredients into 1000mL distilled water at once, heat to dissolve, then use 0.1mol/L sodium hydroxide solution
Adjust the pH to between 7.0 and 7.2, and place it in a pressure steam sterilizer for sterilization at 121°C for 30 minutes.
4.5.2 Solid LB medium
Add 15g agar to 1000mL liquid LB medium, heat to melt, adjust pH with 0.1mol/L sodium hydroxide solution
The value is between 7.0 and 7.2, and then placed in a pressure steam sterilizer for sterilization at 121°C for 30 minutes.
4.6 Reagents
4.6.1 Disinfectant
75% ethanol disinfectant.
4.6.2 Eluent
Normal saline containing 0.85% sodium chloride. Adjust pH with 0.1mol/L sodium hydroxide solution or 0.1mol/L hydrochloric acid solution
The value is between 7.0 and 7.2, and then placed in a pressure steam sterilizer for sterilization at 121°C for 30 minutes.
4.6.3 Culture medium
Liquid LB medium/physiological saline solution, the concentration of E. coli inoculum is 1/500 (volume ratio), golden
The concentration of Staphylococcus chromosome inoculum is 1/100 (volume ratio). In order to facilitate the dispersion of bacteria, 0.2% of sterile surface activity can be added
Agent (such as Tween 80). Adjust the pH value with 0.1mol/L sodium hydroxide solution or 0.1mol/L hydrochloric acid solution to make it in (25
±1) The temperature is between 7.0-7.2 at ℃. After sub-packaging, place it in a pressure steam sterilizer at 121℃ for 30 minutes.
4.7 Detection of bacteria
The tested bacteria should meet the requirements of Table 1.
Note. According to user requirements, other strains can be added as strains for testing, but the strains used need to be managed by the national-level strain preservation
Provided by the management center.
4.8 Preparation and processing of test pieces
4.8.1 Negative control sample
A sterile culture plate with a diameter of 90mm, number A.
4.8.2 Blank control sample
Artificial board or wood (bamboo) without anti-bacterial treatment made of the same material and the same processing technology as the tested sample
The product is cut into a sample with a size of (50±1)mm×(50±1)mm, and the number is B.
4.8.3 Antibacterial treatment of wood-based panels or wood (bamboo) product samples
Select various man-made panels or wood (bamboo) products that have undergone antibacterial treatment, and cut into a size of (50±1) mm×(50±1)
mm sample, numbered C.
4.8.4 Number of samples
The number of samples of each type of the above negative, blank control, and antibacterial treatment should be no less than 6.Surface should be carried out before testing
For disinfection, wipe the surface of the sample with 75% ethanol solution, rinse with sterile water after 1 min, and dry it naturally for later use.
4.9 Testing procedure
4.9.1 Strain preservation
Strains were inoculated on the slant of nutrient medium, cultured at 37±2℃ for 24h, and preserved at 0-5℃ (not more than 1
Month), as a slant to preserve bacteria.
4.9.2 Strain activation
Transfer the slant preserved strains to the plate medium and incubate at 37±2℃ for 24h, transfer once a day, no more than
Two weeks. The fresh bacterial culture transferred within 24 hours should be used for the test.
4.9.3 Preparation of bacterial suspension
Use an inoculation loop to take a small amount (scrape 1-2 loops) of fresh bacteria from the 4.9.2 medium and add it to the inoculation solution. After culturing for 24 h,
Make 10-fold increasing dilutions in sequence, and choose the dilution with the bacterial concentration of 5.0×105 CFU/mL～10.0×105 CFU/mL
As the test bacteria liquid, operate according to the method specified in GB/T 4789.2-2016.
4.9.4 Sample test
Take 0.2 mL test bacterial solution 4.9.3 and drop it on the blank control sample (B) and antibacterial treatment artificial board or wood (bamboo) products.
On the sample (C), at the same time, take 0.2 mL of sterile water and drip it on the negative control sample (A). Use sterile tweezers to pick up the sterile cover
The membrane is respectively covered on sample A, sample B and sample C. It must be spread flat so that the bacteria evenly contact the sample; put it in a sterilized dish,
Incubate for 24 h under the conditions of ±2℃ and relative humidity >90%. Make 3 parallels for each sample.
Take out the samples cultured for 24 hours, add 20 mL of eluent to each piece, and wash sample A, sample B, sample C and cover film repeatedly
(It is best to use tweezers to pick up the film and rinse). After shaking well, take 0.5 mL of the washing solution into a sterilized petri dish, and pour into 15 mL～
In 20 mL of solid medium cooled to 45±2℃, shake well and solidify, incubate at 37℃±2℃ for 24 to 48 hours, then count the activity
For the number of bacteria, operate according to the method specified in GB/T 4789.2-2016.
The above test was repeated once.
4.10 Test result requirements and calculation
4.10.1 Test result requirements
Calculate the actual number of viable bacteria of samples A, B and C after 24 hours of culture according to the dilution factor.
Collect the values of viable bacteria and record them as Na, Nb, and Nc, respectively, to ensure that the test results meet the following requirements at the same time, otherwise the test is invalid.
(A) The negative control should grow aseptically (Na is 0);
(B) The three parallel viable bacteria values of the control sample shall meet the following requirements.
(Highest value-lowest value)/average number ≤ 0.3;
(C) The control sample should not have obvious antibacterial effect, and the actual recovered colony value Nb should not be less than 1.0×104CFU/piece.
4.10.2 Antibacterial rate calculation
Nb. The average number of recovered bacteria in the blank control sample, CFU/tablet;
Nc. The average number of bacteria recovered from antibacterial wood-based panels or wood (bamboo) products, CFU/piece.
5 Antibacterial performance classification
5.1 In the case where the antibacterial rate of the sample calculated by the two bacterial species test is inconsistent, the lower one shall be used as the criterion.
5.2 Antibacterial performance classification standards
The antibacterial performance grading standard shall be carried out according to Table 2.
6 Test report
The test report should give at least the following aspects.
--Test strain, bacterial preservation number, concentration of inoculum solution;
--The condition of sample and control sample;
--Based on the standard;
--Result. Antibacterial rate and antibacterial performance classification;
--Differences from the basic steps and records of observed abnormal phenomena;
-Test unit, test person;
-Test date.
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