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HYT231-2018 English PDF

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HYT231-2018: Extraction and determination of the total lipid in algae
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PDF similar to HYT231-2018


Standard similar to HYT231-2018

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Basic data

Standard ID HY/T 231-2018 (HY/T231-2018)
Description (Translated English) Extraction and determination of the total lipid in algae
Sector / Industry Marine Industry Standard (Recommended)
Classification of Chinese Standard Z17
Classification of International Standard 07.060; 13.060.01
Word Count Estimation 5,589
Date of Issue 2018-02-13
Date of Implementation 2018-05-01
Regulation (derived from) State Oceanic Administration Bulletin No. 1 of 2018 (general No. 42)
Issuing agency(ies) Ministry of Natural Resources of the People's Republic of China

HYT231-2018: Extraction and determination of the total lipid in algae

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Extraction and determination of the total lipid in algae ICS 07.060; 13.060.01 Z17 People's Republic of China Marine Industry Standard Extraction and determination of total algae lipids Published on.2018-02-13 2018-05-01 implementation Released by the State Oceanic Administration

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the State Oceanic Administration. This standard is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283). This standard was drafted. First Institute of Oceanography of the State Oceanic Administration, Institute of Oceanology, Chinese Academy of Sciences, Ocean University of China. The main drafters of this standard. Yang Yujuan, Li Qian, Zheng Minggang, Zheng Li, Han Xiaotian, Liu Tao, Zhu Mingzhuang, Chen Farong. Extraction and determination of total algae lipids

1 Scope

This standard specifies the method for the extraction and determination of total lipids in marine algae. This standard applies to the extraction and determination of total lipids in marine algae. Total lipid extraction and determination in other algae can be used as reference.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Principle of the method

The lipid compound in the algal sample was extracted by a methanol chloroform mixed solution and then determined by a gravimetric method.

4 reagents

Unless otherwise stated, the reagents used in this method are of analytical grade and the water is the tertiary water specified in GB/T 6682. 4.1 Sodium chloride (NaCl). 4.2 anhydrous sodium sulfate (Na2SO4). 4.3 Chloroform (CHCl3). 4.4 Methanol (CH3OH). 4.5 Liquid nitrogen. 4.6 Methanol chloroform mixed solution. prepared according to the volume ratio of methanol chloroform 2.1, prepared before use. 4.7 1% sodium chloride solution. Weigh 1g of sodium chloride (3.1) dissolved in water and dilute to 100mL, valid for 1 month.

5 instruments and equipment

5.1 Electronic balance. Sensing amount 0.1mg. 5.2 Shredder. 5.3 Ultrasonic cleaner (200W). 5.4 Nitrogen blowing instrument. 5.5 Centrifuge (8000r/min). 5.6 Oven. 5.7 Freeze dryer. 5.8 Research and development. 5.9 separatory funnel. 5.10 with a centrifuge tube.

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