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HYT228-2018: Determination of fatty acids in algae-gas chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| HY/T 228-2018 | English | 229 |
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Determination of fatty acids in algae-gas chromatography
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HY/T 228-2018
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Basic data | Standard ID | HY/T 228-2018 (HY/T228-2018) | | Description (Translated English) | Determination of fatty acids in algae-gas chromatography | | Sector / Industry | Marine Industry Standard (Recommended) | | Classification of Chinese Standard | Z17 | | Classification of International Standard | 07.060; 13.060.01 | | Word Count Estimation | 10,111 | | Date of Issue | 2018-02-13 | | Date of Implementation | 2018-05-01 | | Regulation (derived from) | State Oceanic Administration Bulletin No. 1 of 2018 (general No. 42) | | Issuing agency(ies) | Ministry of Natural Resources of the People's Republic of China | HYT228-2018: Determination of fatty acids in algae-gas chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of fatty acids in algae-gas chromatography
ICS 07.060; 13.060.01
Z17
People's Republic of China Marine Industry Standard
Determination of algal fatty acids by gas chromatography
Published on.2018-02-13
2018-05-01 implementation
Released by the State Oceanic Administration
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the State Oceanic Administration.
This standard is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283).
This standard was drafted. First Institute of Oceanography of the State Oceanic Administration, Institute of Oceanology, Chinese Academy of Sciences, Ocean University of China.
The main drafters of this standard. Yang Yujuan, Li Qian, Zheng Minggang, Zheng Li, Han Xiaotian, Liu Tao, Zhu Mingzhuang, Chen Farong.
Determination of algal fatty acids by gas chromatography
1 Scope
This standard specifies the gas chromatographic method for the determination of fatty acids in algae.
This standard applies to the determination of fatty acids in algae. Determination of fatty acids in other algae can be used as reference.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Principle of the method
The fatty acid in the algae sample is saponified with sodium hydroxide in methanol solution and methyl esterified with methanolic hydrochloric acid solution. After extraction with n-hexane, the gas phase is used.
Chromatographic determination.
4 reagents and materials
Unless otherwise stated, the reagents used in this method are of analytical grade and the water is the first grade water specified in GB/T 6682.
4.1 Reagents
4.1.1 Potassium hydroxide (KOH).
4.1.2 Hydrochloric acid (HCl).
4.1.3 Sodium chloride (NaCl).
4.1.4 n-Hexane (C6H14). Chromatographically pure.
4.1.5 Methanol (CH3OH). chromatographically pure.
4.1.6 anhydrous sodium sulfate (Na2SO4). burning at 400 ° C for 4 h, sealed and stored after cooling, valid for 1 month.
4.2 Reagent preparation
4.2.1 Saturated potassium hydroxide methanol solution. Weigh 40g of potassium hydroxide dissolved in 100mL of methanol, stir to dissolve, clarify standby, room temperature
It can be placed for 2 months.
4.2.2 Hydrochloric acid methanol solution (1mol/L). Dissolve 10mL hydrochloric acid in 110mL methanol, mix and store at room temperature for 2 months.
4.2.3 Saturated sodium chloride solution. Weigh 360g of sodium chloride dissolved in 1L of distilled water, stir to dissolve, clarify and reserve, and place at room temperature.
Months.
4.3 Standards
4.3.1 Eleven carbonic acid triester, purity greater than 99%.
4.3.2 Eleven methyl carbonate standard, the purity is greater than 99%.
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