Path: Home > GB > Page176 > GBZ133-2025 || Home > Standard_List > GB > Page176 > GBZ133-2025 Price & DeliveryUS$614.00 ยท In stock
Delivery: <= 5 days. True-PDF full-copy in English will be manually translated and delivered via email.
GBZ133-2025: Nanotechnologies - Assessment of protein secondary structure alterations caused by nanomaterials - Ultraviolet circular dichroism spectroscopy
Status: Valid
GBZ133: Evolution and historical versions
| Std ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status |
| GB/Z 133-2025 | English | 614 |
Add to Cart
|
5 days [Need to translate]
|
Nanotechnologies - Assessment of protein secondary structure alterations caused by nanomaterials - Ultraviolet circular dichroism spectroscopy
| Valid |
| GBZ 133-2009 | English | 439 |
Add to Cart
|
3 days [Need to translate]
|
Radiological protection management for medical radioactive waste
| Obsolete |
Standard similar to GBZ133-2025 WS/T 655 | GB/T 36388 | WS 310.1 | GB/Z 139 | GB/Z 138 | Basic data | Standard ID | GB/Z 133-2025 (GB/Z133-2025) | | Description (Translated English) | Nanotechnologies - Assessment of protein secondary structure alterations caused by nanomaterials - Ultraviolet circular dichroism spectroscopy | | Sector / Industry | National Standard | | Classification of Chinese Standard | C04 | | Classification of International Standard | 07.120 | | Word Count Estimation | 30,391 | | Date of Issue | 2025-12-03 | | Date of Implementation | 2025-12-03 | | Issuing agency(ies) | State Administration for Market Regulation, Standardization Administration of China | GB/Z 133-2025: Nanotechnologies - Assessment of protein secondary structure alterations caused by nanomaterials - Ultraviolet circular dichroism spectroscopy
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
GB /Z 133-2025.Evaluation of Changes in Protein Secondary Structure Caused by Nanotechnology and Nanomaterials - Ultraviolet Circular Dichroism Spectroscopy
ICS 07.120
CCSC04
National Standardization Guiding Technical Documents of the People's Republic of China
Nanotechnology and nanomaterials lead to changes in protein secondary structure
Change assessment using ultraviolet circular dichroism spectroscopy
Published on 2025-12-03
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Table of contents
Preface III
Introduction IV
1.Scope 1
2 Normative References 1
3.Terms and Definitions 1
4.Abbreviations 2
5.Interactions between nanomaterials and proteins 2
6.Sample Preparation 3
6.1 General Rules 3
6.2 Performance Requirements of UV-CD Quartz Cuvettes 3
6.3 Preparation of Protein Solutions 3
6.4 Instrument Condition Settings 3
6.5 UV-CD Spectrum Acquisition Steps 3
6.5.1 Overview 3
6.5.2 Buffer solution 4
6.5.3 Protein Sample 4
6.5.4 Stability of Nanoparticle Suspensions in Protein Solutions 4
6.6 Preparation of Protein-Nanoparticle Complex Suspension 4
6.7 UV-CD Spectroscopy Measurement 5
6.8 MRE Calculation 5
6.9 Data Analysis 5
7.Test Report 6.
Appendix A (Informative) Typical Characteristics of Protein UV-CD Spectra 7
Appendix B (Informative) Literature Search on NOAA and Protein Structural Changes 8
Appendix C (Informative) Description of Buffer Solutions that Can Be Used for Protein Solubility 14
C.1 Buffer solution 14
C.2 Cleaning Agent 14
C.3 Absorption of nanoparticles in CD experiment 15
C.4 Absorption of buffer solution in the far ultraviolet region 16
C.5 Sample preparation control and acquisition of high-quality spectra 16
Appendix D (Informative) Unit Conversion in CD Measurements 17
Appendix E (Informative) Calculation of Sample Concentration Range 18
Appendix F (Informative) Methods for assessing protein secondary structure 19
Appendix G (Informative) Typical Data for Evaluating Protein Secondary Structure Using UV-CD 20
References 21
Foreword
This document is a standard or guiding technical document.
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
This document is equivalent to ISO /T S23459.2021 "Nanotechnology - Evaluation of protein induced by nanomaterials using ultraviolet circular dichroism spectroscopy".
The document type of "Changes in Secondary Structure of Proteins" has been changed from an ISO technical specification to a national standardization guidance technical document of my country.
The following minimal editorial changes have been made to this document.
---To align with existing standards, the standard name has been changed to "Evaluation of Changes in Protein Secondary Structure Caused by Nanotechnology and Nanomaterials (Purple)".
"Outer Circular Dichromatic Spectroscopy";
---The error in equation (2) of ISO /T S23459.2021 has been corrected.
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the Chinese Academy of Sciences.
This document is under the jurisdiction of the National Technical Committee on Standardization of Nanotechnology (SAC/TC279).
The main drafting units of this document are. National Center for Nanoscience and Technology, Institute of High Energy Physics, Chinese Academy of Sciences, and Basic Medical Sciences, Chinese Academy of Medical Sciences.
Research Institute, Jiageng Innovation Laboratory, Shenzhen Institute of Standards and Technology.
The main drafters of this document are. Wu Xiaochun, Ji Yinglu, Zhang Chenqi, Li Hanbo, Zhou Jin, Wang Liming, Wen Tao, Xu Haiyan, Cao Lingyun, and Wang Yasi.
Wang Yiqun and Fan Yangbo.
Introduction
Currently, nanomaterials (such as nanoobjects and their aggregates and clusters, NOAA) are being mass-produced and widely used globally.
They are found in various fields. However, there are concerns about their interactions with biological systems (including proteins), which could potentially lead to...
This can lead to reversible or irreversible changes in the secondary structure of a protein. The latter may affect the protein's function and conformation, thereby influencing the protein as a whole.
Biological reactivity. Monitoring these changes could provide important information about the interactions between NOAA and biological systems.
In biological matrices, the folding process of polypeptides produces the secondary structure of proteins, which determines their biological activity. An important feature of this structure is...
This includes hydrogen bonds between the amino hydrogen and carbonyl oxygen atoms on the peptide backbone and disulfide bonds between two cysteine residues.
Certain metal ions and bioactive compounds can affect the secondary structure of proteins. Furthermore, the ionic strength, pH, and other properties of different buffer solutions can also influence this.
Temperature also has an effect [1]. Changes in protein function and conformation may arise from reorganization during the folding process (i.e., misfolding) and the overall protein structure.
Changes in protein size. Some diseases, such as amyotrophic lateral sclerosis (ALS), Alzheimer's disease, and Parkinson's disease, are caused by the misfolding of proteins.
Caused by stacking [2].
Several methods exist for determining NOAA-induced structural and conformational changes in protein molecules, as well as folding processes, including high-field methods.
Nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, Raman spectroscopy, and ultraviolet circular dichroism (UV-CD) spectroscopy [3-6].
In addition, synchrotron circular dichroism spectroscopy (SRCD) is a sensitive method that can provide information about protein secondary structure and folding.
Information [7].
Nanotechnology and nanomaterials lead to changes in protein secondary structure
Change assessment using ultraviolet circular dichroism spectroscopy
1 Scope
This document provides a method for evaluating protein secondary structure resulting from interactions with nanomaterials using ultraviolet circular dichroism (UV-CD) spectroscopy.
Changing test plans and conditions.
This document is not applicable to characterizing conformational changes in disordered proteins.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
ISO 80004-1 Nanotechnology Vocabulary Part 1.Core Terms
Corevocabulary)
Note. GB/T 30544.1-2014 Nanotechnology Terminology Part 1.Core Terminology (ISO /T S80004-1.2010, IDT)
ISO /T S80004-2 Nanotechnology Vocabulary Part 2.Nanomaterials
Nano-objects
ISO /T S80004-4 Nanotechnology Terminology Part 4.Nanostructured Materials
Note. GB/T 30544.4-2019 Nanotechnology Terminology Part 4.Nanostructured Materials (ISO /T S80004-4.2011, IDT)
ISO /T S80004-6 Nanotechnology Terminology Part 6.Characterization of Nanomaterials
Note. GB/T 30544.6-2016 Nanotechnology Terminology Part 6.Characterization of Nanomaterials (ISO /T S80004-6.2013, MOD)
3 Terms and Definitions
The terms and definitions defined in ISO 80004-1, ISO /T S80004-2, ISO /T S80004-4 and ISO /T S80004-6, as well as the following terms and definitions.
This applies to this document.
3.1
nanoparticles;NP
A nano-object with three external dimensions at the nanoscale.
Note. If the three external dimensions differ significantly (usually more than 3 times), it is preferable to use terms such as nanofibers or nanosheets rather than nanoparticles.
[Source. ISO 80004-1.2023, 3.3.4]
3.2
nanomaterials
Materials with one or more external dimensions at the nanoscale or containing nanoscale internal or surface structures.
Note 1.This general term includes nanoobjects and nanostructured materials.
Note 2.See engineered nanomaterials, artificial nanomaterials and associated nanomaterials.
[Source. ISO 80004-1.2023, 3.1.4]
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GBZ133-2025_English be delivered?Answer: Upon your order, we will start to translate GBZ133-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 3 ~ 5 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GBZ133-2025_English with my colleagues?Answer: Yes. The purchased PDF of GBZ133-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. Question 5: Should I purchase the latest version GBZ133-2025?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GBZ133-2025 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |