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GB/T 42114-2022: Determination of flavonol in cassava leaves - High-performance liquid chromatography
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Determination of flavonol in cassava leaves - High-performance liquid chromatography
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GB/T 42114-2022
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Basic data | Standard ID | GB/T 42114-2022 (GB/T42114-2022) | | Description (Translated English) | Determination of flavonol in cassava leaves - High-performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 10,146 | | Date of Issue | 2022-12-30 | | Date of Implementation | 2023-07-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 42114-2022: Determination of flavonol in cassava leaves - High-performance liquid chromatography
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67.050
CCSX04
National Standards of People's Republic of China
Determination of flavonols in cassava leaves
HPLC
Posted on 2022-12-30
2023-07-01 implementation
State Administration for Market Regulation
Released by the National Standardization Management Committee
foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules for Standardization Documents"
drafting.
Please note that some contents of this document may refer to patents. The issuing agency of this document assumes no responsibility for identifying patents.
This document is proposed and managed by China National Institute of Standardization.
This document was drafted by. Institute of Tropical Crops Variety Resources, Chinese Academy of Tropical Agricultural Sciences, Zhejiang Academy of Agricultural Sciences, China Standardization
Research Institute, Zhejiang University, Institute of Spice and Beverage, Chinese Academy of Tropical Agricultural Sciences.
The main drafters of this document. Wang Qinfei, Zhang Zhenwen, Lu Baiyi, Yang Li, Sun Caixia, Zhang Yanjun, Lin Liming, Yu Houmei, Lu Xiaojing, Wei Zhuowen,
Li Kaimian.
Determination of flavonols in cassava leaves
HPLC
1 Scope
Determine the method.
This document is applicable to the determination of 4 kinds of flavonols content of myricetin, rutin, nicotinic acid and narcissin in cassava fresh leaves and cassava dry leaves.
2 Normative references
The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references
For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and Definitions
This document does not have terms and definitions that need to be defined.
4 principles
Cassava leaf samples were added with 50% ethanol aqueous solution, ultrasonically assisted extraction, high performance liquid chromatography separation, ultraviolet detector or two
Electrode array detector detection, according to retention time qualitative and external standard method quantitative.
5 Reagents and Materials
Unless otherwise specified, only analytical reagents and grade-1 water in accordance with GB/T 6682 are used in the analysis.
5.1 Reagents
5.1.1 Methanol (CH3OH, CAS No.. 67-56-1). chromatographically pure.
5.1.2 Absolute ethanol (CH3CH2OH, CAS No.. 64-17-5). chromatographically pure.
5.1.3 Phosphoric acid (H3PO4, CAS number. 7664-38-2).
5.2 Reagent preparation
5.2.1 50% ethanol aqueous solution (volume fraction). take 500mL of absolute ethanol (5.1.2), add 500mL of water, and mix well.
5.2.2 0.2% phosphoric acid aqueous solution (volume fraction). Take 2.0mL phosphoric acid (5.1.3), put it in a 1000mL volumetric flask, and dilute to
Degree, ready to use.
5.3 Standards
5.3.1 Myricitin (myricetin-3-O-rutinoside, C27H30O17, CAS No.. 41093-68-9). purity ≥ 98.0%.
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