GB/T 30636-2014 PDF English
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB/T 30636-2014 | English | 150 |
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Determination of sialic acid in swiftlets nest and swiftlets nest products -- Liquid chromatographic method
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GB/T 30636-2014: Determination of sialic acid in swiftlets nest and swiftlets nest products -- Liquid chromatographic method
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT30636-2014
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 67.050
X 04
Determination of sialic acid in swiflets nest and
swiflets nest products - Liquid chromatographic
method
Issued on. DECEMBER 31, 2014
Implemented on. MAY 01, 2015
Issued by. General Administration of Quality Supervision Inspection and
Quarantine of PRC;
Standardization Administration of PRC.
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative references... 4
3 Method summary... 4
4 Reagents and materials... 5
5 Instruments... 5
6 Analytical procedures... 6
7 Determination... 7
8 Result calculation... 7
9 Precision... 8
Appendix A (Informative) Chromatograms of sialic acid standard solution and
swiflets nest sample hydrolysate... 9
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the National Food Industry Standardization
Technical Committee (SAC/TC 64).
This standard shall be under the jurisdiction of the National Food Industry
Standardization Technical Committee Food General Testing Technical
Subcommittee (SAC/TC 64/SC 8).
Drafting organizations of this standard. National Agricultural Deep-Processing
Product Quality Supervision and Inspection Center, Jilin Province Product
Quality Supervision and Inspection Institute.
The main drafters of this standard. Li Gang, Liu Junhui, Li Jieyi, Hua Lei, Li
Yuanyuan, Wang Wei, Wang Ying, Shi Yanyu, An Wei, Wang Qingfeng, Li Dan,
Li Ning.
Determination of sialic acid in swiflets nest and
swiflets nest products - Liquid chromatographic
method
1 Scope
This standard specifies the method for the determination of sialic acid in swiflets
nest and its products by liquid chromatography.
This standard applies to the determination of sialic acid in swiflets nest and its
products.
The detection limit of this standard is 0.3 g/kg for swiflets nest and 0.003 g/kg
for swiflets nest products.
2 Normative references
The following documents are essential to the application of this document. For
the dated documents, only the versions with the dates indicated are applicable
to this document; for the undated documents, only the latest version (including
all the amendments) are applicable to this standard.
GB/T 6379.2 Accuracy (trueness and precision) of measurement methods
and results - Part 2.Basic method for the determination of repeatability and
reproducibility of a standard measurement method
GB/T 6682 Water for analytical laboratory use - Specification and test
methods
3 Method summary
Swiflets nest and swiflets nest products are heated and hydrolyzed in acetic
acid solution to release sialic acid.
4 Reagents and materials
4.1 Water. Meet the grade-1 water requirements as specified in GB/T 6682.
4.2 Acetonitrile. Chromatographically pure.
4.3 Glacial acetic acid. Analytically pure.
4.7 0.1% phosphoric acid solution. Take 1 mL of phosphoric acid; add water to
make the volume to 1000 mL; mix well; use 0.45 μm microporous membrane
to filter it.
4.8 50% acetic acid solution. Take 100 mL of glacial acetic acid; add 100 mL of
water; mix it uniformly.
4.9 10% polyethylene glycol solution. Weigh 50 g of polyethylene glycol; add
500 mL of water and stir to dissolve it.
4.10 Standard stock solution of sialic acid (1.00 mg/mL). Weigh 100.0 mg of
sialic acid; add 20 mL of water to dissolve it; add acetonitrile to make the volume
reach to 100 mL. Store in the dark at 4 °C; the shelf life is one month.
4.11 Dialysis bag. It allows the penetration of compounds which have a
molecular weight of less than 10000; soak in water for 1 h before use.
4.12 0.45 μm microporous membrane. Organic phase.
4.13 0.45 μm syringe filter. Organic phase.
5 Instruments
5.1 Liquid chromatograph. Equipped with ultraviolet detector.
5.2 Constant temperature water bath.
5.3 Mortar.
5.4 Homogenizer. Rotation speed is 10000 r/min.
5.5 Oven.
6 Analytical procedures
6.1 Specimen processing
6.1.1 Dried swiflets nest
Take an appropriate amount of specimen and dry it at 105 °C for 60 minutes.
Cool it in a desiccator.
6.1.2 Swiflets nest products with rock sugar
Take an appropriate amount of specimen in a beaker. Homogenize at a speed
of 10000 r/min for 3 minutes.
6.2 Preparation of sialic acid series standard working solution
Measure 1.00 mL, 2.00 mL, 4.00 mL, 6.00 mL, 8.00 mL, 10.0 mL of the standard
stock solution of sialic acid, respectively. Place them in a 100 mL volumetric
flask. Add the mobile phase to the mark.
7 Determination
7.1 Chromatographic reference conditions
7.1.1 Chromatographic column. 300SCX cation exchange chromatographic
column, 4.6 mm × 250 mm, particle size 5 μm, or equivalent.
7.1.2 Mobile phase. Acetonitrile-0.1% phosphoric acid aqueous solution (90 +
10, volume ratio).
7.1.6 Detection wavelength. 205 nm.
7.2 Liquid chromatography determination
Take the sialic acid series standard solution and test solution, respectively. Use
the peak area of the standard series solution as the ordinate and the
concentration as the abscissa, draw the standard curve.
8 Result calculation
The measurement results can be automatically calculated by the instrument
workstation, or calculated according to formula (1).
9 Precision
9.1 General provisions
The precision of this standard is determined in accordance with the provisions
of GB/T 6379.2.The repeatability and reproducibility values are calculated with
95% confidence.
9.2 Repeatability
Under repeatability conditions, the absolute difference between two
independent test results as obtained does not exceed the repeatability limit (r).
The content range and repeatability equation of different samples are as shown
in Table 1.
9.3 Reproducibility
Under the condition of reproducibility, the absolute difference between the two
independent test results as obtained does not exceed the reproducibility limit
(R). The content range and reproducibility equation of different samples are as
shown in Table 1.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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