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GB/T 40643-2021: Determination of hyperoside in extract of crataegus pinnatifida leaf - HPLC
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Determination of hyperoside in extract of crataegus pinnatifida leaf - HPLC
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GB/T 40643-2021
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PDF similar to GB/T 40643-2021
Basic data | Standard ID | GB/T 40643-2021 (GB/T40643-2021) | | Description (Translated English) | Determination of hyperoside in extract of crataegus pinnatifida leaf - HPLC | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B04 | | Word Count Estimation | 6,662 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40643-2021: Determination of hyperoside in extract of crataegus pinnatifida leaf - HPLC---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of hyperoside in extract of crataegus pinnatifida leaf - HPLC
ICS 65.020.01
CCSB04
National Standards of People's Republic of China
Determination of Hyperin in Hawthorn Leaf Extract
High performance liquid chromatography
HPLC
Released on 2021-10-11
2022-05-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed by the State Forestry and Grassland Administration.
This document is under the jurisdiction of the National Forest Chemical Products Standardization Technical Committee (SAC/TC558).
Drafting organizations of this document. Beijing Forestry University, China National Institute of Standardization, Research Institute of Forest Products Chemical Industry, Chinese Academy of Forestry, Beijing
Beijing Electronic Technology Vocational College, Zhejiang Shengshi Biological Technology Co., Ltd., Zhejiang University of Science and Technology, Shenzhen Brand Building Promotion Center, Zhongshan Hongli
Health Food Industry Research Institute, Hebei Guanzhuo Testing Technology Co., Ltd., Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Infinitus
(China) Co., Ltd. is common.
The main drafters of this document. Lei Jiandu, Xi Xingjun, Liu Jing, Wang Luying, Wang Xiaoxiao, Yang Zhihua, Gong Qizhou, Zhang Jingxuan, Liu Tiebing, Xin Xiulan,
Zhao Xinxin, Di Duolong, Pei Dong, Li Wenjun, Lan Tao, Chen Liang, Liu Fengsong, Sun Hongmei.
Determination of Hyperin in Hawthorn Leaf Extract
High performance liquid chromatography
1 Scope
This document describes the principle, instruments and equipment, reagents and materials for the determination of hyperoside in hawthorn leaf extract by high performance liquid chromatography.
Materials, testing methods, calculation and presentation of results, repeatability, precision, and recovery rate of standard addition.
This document is applicable to the determination of hyperoside content in hawthorn leaf extract.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Principle
In the same analysis cycle, adjust the mobile phase ratio according to a certain time program to make the key components of the hawthorn leaf extract according to their respective appropriate
The capacity factor achieves a good separation purpose.
The external standard method corresponding to hyperoside was used for direct quantification, and the chromatographic column used was a reversed-phase C18 column. Ultraviolet light of hyperin
Spectral measurement showed that the maximum absorption wavelength was 360nm, so 360nm was selected as the detection wavelength of hyperoside by high performance liquid chromatography.
5 Reagents and materials
Unless otherwise specified, only reagents of analytical purity and water that meet GB/T 6682 level 1 are used in the analysis.
5.1 Reagents
5.1.1 Methanol (CH3OH), chromatographically pure.
5.1.2 Glacial acetic acid (CH3COOH).
5.2 Standard products
Hyperin standard product. purity ≥98%.
5.3 Standard stock solution of hyperoside
Weigh.200mg (accurate to 0.001g) hyperoside standard substance in a 100mL brown volumetric flask, add methanol to dissolve and dilute to
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