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GB/T 40368-2021: Determination of kunitz trypsin inhibitor of plant metabolites - Enzyme-linked immunosorbent assay
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| GB/T 40368-2021 | English | 119 |
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Determination of kunitz trypsin inhibitor of plant metabolites - Enzyme-linked immunosorbent assay
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GB/T 40368-2021
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Basic data | Standard ID | GB/T 40368-2021 (GB/T40368-2021) | | Description (Translated English) | Determination of kunitz trypsin inhibitor of plant metabolites - Enzyme-linked immunosorbent assay | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Word Count Estimation | 6,685 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40368-2021: Determination of kunitz trypsin inhibitor of plant metabolites - Enzyme-linked immunosorbent assay
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Determination of kunitz trypsin inhibitor of plant metabolites - - Enzyme-linked immunosorbent assay
ICS 07.080
CCSA40
National Standards of People's Republic of China
Determination of trypsin inhibitor of plant metabolites
Enzyme-linked immunosorbent assay
Released on 2021-08-20
2022-03-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387).
Drafting organizations of this document. Jiangnan University, Beijing Technology and Business University, Beijing Sambo Technology Co., Ltd., Lishui Lancheng Agricultural Science Testing Technology Co., Ltd.
company.
The main drafters of this document. Ma Aijin, Kuang Hua, Hao Shuai, Liu Liqiang, Xu Xinxin, Lin Lu, Zhang Shuqi.
Determination of trypsin inhibitor of plant metabolites
Enzyme-linked immunosorbent assay
1 Scope
This document specifies the enzyme-linked immunosorbent assay method for plant metabolite trypsin inhibitor.
This document is applicable to the determination of trypsin inhibitor of the metabolite of soybean and its products.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Principle
After the sample is processed, the trypsin inhibitor in the sample solution is bound to the trypsin inhibitor antibody (capture antibody) coated in the microplate.
Combine, wash the plate to remove unbound other components, and then combine with the enzyme-labeled antibody of trypsin inhibitor, add the enzyme-labeled substrate to develop the color, and use the microplate reader
Measure the absorbance value at 450nm wavelength and calculate the trypsin inhibitor content.
5 Reagents and materials
Unless otherwise specified, the chemical reagents used in this method are analytically pure, and the water is secondary water specified in GB/T 6682.
5.1 Trypsin Inhibitor Detection Kit. See Appendix A.
5.2 Trypsin inhibitor. purity ≥95%, CAS number is 9035-81-8.
5.3 The required solution should be prepared in accordance with the instructions of the kit.
6 Equipment
6.1 Small grinder.
6.2 Sample sieve. the aperture is 0.9mm.
6.3 Homogenizer.
6.4 Vortex mixer.
6.5 Electronic balance. a sense of 0.01g.
6.6 Centrifuge tube with stopper. 15mL.
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