GB/T 40179-2021 PDF English
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Determination of organic acids in plant - Liquid chromatography-tandem mass spectrometry
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GB/T 40179-2021: Determination of organic acids in plant - Liquid chromatography-tandem mass spectrometry
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT40179-2021
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 07.080
CCS A 40
Determination of organic acids in plant - Liquid
chromatography-tandem mass spectrometry
ISSUED ON: MAY 21, 2021
IMPLEMENTED ON: DECEMBER 01, 2021
Issued by: State Administration for Market Regulation;
Standardization Administration of PRC.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Terms and definitions ... 4
4 Principle ... 4
5 Reagents or materials ... 5
6 Equipment ... 6
7 Analytical procedures ... 6
8 Processing of test data ... 9
9 Precision ... 9
10 Others ... 9
Appendix A (Informative) Information and limit of quantification of organic acid
compound ... 10
Appendix B (Informative) Reference conditions for liquid chromatography-
tandem/mass spectrometry parameter ... 11
Appendix C (Informative) Liquid chromatography-tandem/mass spectrometry
MRM diagrams of 15 organic acids ... 13
Determination of organic acids in plant - Liquid
chromatography-tandem mass spectrometry
1 Scope
This document specifies the method for the determination of organic acids in
plants, by liquid chromatography-tandem/mass spectrometry.
This document applies to the determination of fumaric acid, trans-aconitic acid,
adipic acid, gallic acid, vanillic acid, chlorogenic acid, caffeic acid, syringic acid,
p-coumaric acid, ferulic acid, p-hydroxybenzoic acid, salicylic acid, 2,5-
dihydroxybenzoic acid, 3,4-dihydroxybenzoic acid, in citrus, apple, strawberry,
cucumber, tomato, honeysuckle, codonopsis, hawthorn, medlar.
2 Normative references
The contents of the following documents constitute the indispensable clauses
of this document through normative references in the text. Among them, for
dated reference documents, only the version corresponding to that date is
applicable to this document; for undated reference documents, the latest
version (including all amendments) is applicable to this document.
GB/T 6682 Water for analytical laboratory use - Specification and test
methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Organic acids
An acidic organic compound in plant materials.
4 Principle
After the specimen is heated and refluxed by 50% methanol solution, it is
separated by reversed-phase chromatographic column, determined and
5.9 Internal standard solution: Accurately pipette 1.0 mL of the internal standard
stock solution, into a 100 mL volumetric flask. Use 50% methanol solution to
dilute it, to make the volume to the mark. Mix well, to prepare an internal
standard solution, which has a concentration of 1.0 mg/L. Prepare it before use.
5.10 Mixed standard working solution: Accurately pipette an appropriate
amount of mixed standard stock solution, into a 10 mL volumetric flask. Then
accurately add 2.5 mL of internal standard solution respectively. Use 50%
methanol solution to dilute it, to prepare the series of mixed standard working
solutions, which have a concentration of 0.10 mg/L, 0.25 mg/L, 0.50 mg/L, 0.75
mg/L, 1.0 mg/L, 1.25 mg/L. Prepare it before use.
5.11 0.22 μm hydrophilic nylon microporous filter membrane.
6 Equipment
6.1 Liquid chromatography-tandem/mass spectrometer: Equipped with
electrospray ion source (ESI).
6.2 Electronic balance: Sensitivity is 0.0001 g and 0.01 mg.
6.3 High-speed centrifuge: The speed is not less than 10000 r/min.
6.4 High-speed homogenizer.
6.5 High-speed crusher.
6.6 Condensation reflux device.
7 Analytical procedures
7.1 Sample preparation
Take about 1 kg of representative dried samples of honeysuckle, codonopsis,
hawthorn, wolfberry, etc. Use a crusher to crush it. Mix well. Divide the prepared
specimen equally into two sets. Contain them in clean vessels. Seal and mark
it. Store it at 0 °C ~ 4 °C. Make determination within one week.
Take about 1 kg of representative fresh samples of citrus, apples, strawberries,
cucumbers, tomatoes, etc. Use a homogenizer to homogenize them. Divide the
prepared specimen equally into two sets. Contain them in clean vessels. Seal
and mark it. Prepare it before use.
7.2 Processing of specimen
Weigh about 1.5 g of dry sample or 10 g of fresh sample (accurate to 0.001 g),
Perform two parallel determinations, on the same specimen, according to the
provisions of 7.2 ~ 7.4.
8 Processing of test data
The calculation of the content of the analyte, in the sample, is as shown in
formula (1):
Where:
X - The content of the tested component in the specimen, in milligrams per
kilogram (mg/kg);
ρ - The mass concentration of the tested component solution, which is
obtained from the standard working curve of internal standard, in
micrograms per milliliter (μg/mL);
V - The total volume of the sample solution, in milliliter (mL);
m - The mass of the specimen, in grams (g).
The calculation result is expressed as the arithmetic mean of two independent
determination results, which are obtained under repeatability conditions. The
result retains to two decimal places.
9 Precision
The absolute difference between two independent determination results, which
are obtained under repeatability conditions, shall not exceed 10% of the
arithmetic mean.
10 Others
Refer to Table A.1 in Appendix A for the limits of quantification of 14 organic
acids, which are determined by this method.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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