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GB/T 39730-2020: General requirements for cell counting - Flow cytometry
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 39730-2020 | English | 189 |
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General requirements for cell counting - Flow cytometry
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GB/T 39730-2020
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PDF similar to GB/T 39730-2020
Standard similar to GB/T 39730-2020 GB/T 41907 GB/T 44828 GB/T 39729 Basic data | Standard ID | GB/T 39730-2020 (GB/T39730-2020) | | Description (Translated English) | General requirements for cell counting - Flow cytometry | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,139 | | Date of Issue | 2020-12-14 | | Date of Implementation | 2021-07-01 | | Regulation (derived from) | National Standard Announcement No. 28 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 39730-2020: General requirements for cell counting - Flow cytometry---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(General requirements for cell counting Flow cytometry)
ICS 07:080
CCSC27
National Standards of People's Republic of China
General requirements for cell counting
Flow cytometry
2020-12-14 release
2021-07-01 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Table of contents
Foreword Ⅰ
Introduction Ⅱ
1 Scope 1
2 Normative references 1
3 Terms, definitions and abbreviations 1
4 Method principle 2
5 General requirements 2
5:1 Reagents or materials 2
5:2 Equipment 3
5:3 Sample preparation 3
5:4 Test Step 4
5:5 Data Analysis 6
5:6 Method confirmation 6
5:7 Report 6
Reference 7
Foreword
This document is in accordance with the provisions of GB/T 1:1-2020 Standardization Guidelines Part 1: Structure and Drafting Rules of Standardization Documents
Drafting:
Please note that certain contents of this document may involve patents: The issuing agency of this document is not responsible for identifying patents:
This document was proposed and managed by the National Standard Material Research Center:
Drafting organization of this document: Chinese Academy of Metrology:
The main drafters of this document: Liu Yingying, Wang Jing, Fu Boqiang, Yuan Baozhu, Niu Chunyan, Sui Zhiwei, Chen Chuan:
Introduction
Flow cytometry is a technique for high-speed, multi-parameter analysis of single particles or cells suspended in liquid: Can be opposed by flow cytometry
Direct counting of the cells in the sample preparation can not only achieve the total cell count in the sample, but also perform typing and counting according to cell characteristics:
number: In order to achieve data consistency between measurement system conditions such as different laboratories, platforms, operators, etc:, it is necessary to perform flow cytometry
Standardize the technical aspects of the cell counting test:
General requirements for cell counting
Flow cytometry
1 Scope
This document describes the terms, definitions, abbreviations, method principles, and reagents or materials, instrument
General requirements for equipment, sample preparation, test procedures, data analysis, method validation and reporting:
This document is applicable to cell counting performed by flow cytometry in research, production and testing:
2 Normative references
There are no normative references in this document:
3 Terms, definitions and abbreviations
3:1 Terms and definitions
The following terms and definitions apply to this document:
3:1:1
Celcounting
Measurement of cell number:
[Source: ISO 20391-1:2018,3:8]
3:1:2
Cell staining
A technique in which specific components of cells are combined with fluorescent dyes or specific antibodies labeled with fluorescent dyes for detection:
Note: The specific components are protein, DNA, RNA, etc:
3:1:3
Certified reference material; CRM
Attached with documents issued by an authoritative organization, providing one or more characteristics with uncertainty and traceability obtained using effective procedures
The value of the standard substance:
[Source: JJF1001-2018, 8:15]
3:1:4
Accuracy
Measurementaccuracy
The degree of agreement between the measured value and its true value:
[Source: JJF1001-2018, 5:8, with modification]
3:1:5
Precision
Measurementprecision
Under specified conditions, the degree of agreement between the displayed value or the measured value of the same or similar measured object:
[Source: JJF1001-2018, 5:10, with modification]
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