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GB/T 39602-2020: Diagnostic techniques for Lumpy skin disease
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Diagnostic techniques for Lumpy skin disease
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GB/T 39602-2020
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Basic data | Standard ID | GB/T 39602-2020 (GB/T39602-2020) | | Description (Translated English) | Diagnostic techniques for Lumpy skin disease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 19,112 | | Date of Issue | 2020-12-14 | | Date of Implementation | 2020-12-14 | | Quoted Standard | GB/T 6682; GB 19489; NY/T 541 | | Regulation (derived from) | National Standard Announcement No. 28 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the clinical diagnosis, laboratory diagnostic techniques and procedures for bovine nodular skin disease (LSD). This standard applies to the diagnosis of bovine nodular skin diseases. Virus isolation and identification is suitable for the no-infection certificate or clinical case confirmation before the movement of individual animals; electron microscope observation is suitable for the diagnosis of clinical cases; real-time fluorescent PCR and ordinary PCR are suitable for the no-infection certificate, clinical case confirmation and monitoring of infection before the movement of individual animals Prevalence rate; Serum neutralization test is suitable for proof of no infection, confirmed clinical cases, monitoring infection prevalence rate and immune effect evaluation before individual animals move. | GB/T 39602-2020: Diagnostic techniques for Lumpy skin disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(The diagnosis technology of bovine nodular skin disease)
ICS 11:220
B41
National Standards of People's Republic of China
The diagnosis technology of bovine nodular skin disease
2020-12-14 release
2020-12-14 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Table of contents
Preface Ⅲ
Introduction Ⅳ
1 Scope 1
2 Normative references 1
3 Abbreviations 1
4 Clinical diagnosis 1
4:1 Susceptible animals 1
4:2 Clinical symptoms 2
4:3 Pathological changes 2
4:4 Results judgment 2
5 Laboratory diagnostic sample collection 2
5:1 Equipment 2
5:2 Reagent 2
5:3 Sample collection 3
5:4 Inspection and storage of samples 3
5:5 Sample processing 3
6 Electron microscope observation 4
6:1 Equipment 4
6:2 Reagent 4
6:3 Step 4
6:4 Results judgment 4
7 Virus isolation and identification 4
7:1 Equipment 4
7:2 Cells and reagents 4
7:3 Virus isolation 5
7:4 Virus identification 5
8 Real-time fluorescent polymerase chain reaction (real-time fluorescent PCR) 5
8:1 Equipment 5
8:2 Reagent 5
8:3 Primers and probes 5
8:4 Standard strains and cells 6
8:5 Processing of samples 6
8:6 Extraction and purification of viral DNA 6
8:7 Real-time fluorescent PCR detection 6
8:8 Results judgment 7
9 Polymerase chain reaction (general PCR method) 7
9:1 Equipment 7
9:2 Reagent 7
9:3 Primer 8
9:4 Preparation of samples 8
9:5 PCR detection 8
9:6 Results judgment 9
10 Minimal Neutralization Test (VN) 9
10:1 Equipment 9
10:2 Cell line 9
10:3 Standard strain 9
10:4 Operation steps 9
10:5 Negative and positive control 10
10:6 Result judgment 10
10:7 Interpretation of results 10
11 Comprehensive decision 10
11:1 Suspected 10
11:2 Confirmed 10
Appendix A (Normative Appendix) Reagent Preparation 11
Appendix B (informative appendix) primer amplification sequence 14
Foreword
This standard was drafted in accordance with the rules given in GB/T 1:1-2009:
This standard corresponds to 3:4:12 bovine nodular skin in the latest OIE's "Manual of Diagnostic Tests and Vaccines for Terrestrial Animals" (2018 Edition)
Disease (LSD) related content, and the degree of consistency with the standard is not equivalent:
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China:
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181):
Drafting organizations of this standard: Chongqing Customs of the People's Republic of China, China Animal Health and Epidemiology Center, Shanghai, People's Republic of China
Customs, Chongqing Aolong Biological Products Co:, Ltd:
The main drafters of this standard: Nie Fuping, Li Lin, Li Yingguo, Wu Xiaodong, Wang Yu, Yang Jun, Fan Xiaoxu, Wang Guomin, Li Xianliang, Nan Wenlong,
Shi Meimei, Zhang Lei, Zou Yanli, Wang Zhiliang, Li Jian, Ran Zhiguang:
Introduction
The issuing agency of this document draws attention to the fact that when declaring compliance with this document, primer pair 2 and probe 2 in 8:3 primers and probes may be involved
Comparing with "Primers, Kits and Detection Methods for Bovine Nodular Skin Disease Virus Field Strain TaqMan-MGB Real-time Fluorescent Quantitative PCR Detection"
Use of related patents:
The issuing agency of this document has no position on the authenticity, validity and scope of the patent:
The patent holder has assured the issuing organization of this document that he is willing to work with any applicant under reasonable and non-discriminatory terms and conditions:
Negotiations on patent licensing: The statement of the patent holder has been filed with the issuing agency of this document: Relevant information can be through the following
Contact information:
Names of patent holders: Nie Fuping, Wang Yu, Yang Jun, Li Xianliang, Wang Guomin, Li Yingguo:
Address: No: 8 Honghuang Road, Jiangbei District, Chongqing:
Please note that in addition to the above patents, certain contents of this document may still involve patents: The issuing agency of this document is not responsible for identifying these
Li’s responsibility:
The diagnosis technology of bovine nodular skin disease
1 Scope
This standard specifies the clinical diagnosis and laboratory diagnostic techniques and procedures for bovine nodular skin disease (LSD):
This standard applies to the diagnosis of bovine nodular skin diseases: Virus isolation and identification is suitable for the non-infection certificate or clinical
Confirmation of bed cases; Electron microscope observation is suitable for diagnosis of clinical cases; Real-time fluorescent PCR and ordinary PCR are suitable for the insensitivity of individual animals before moving
Infection certificate, clinical case diagnosis and monitoring of infection prevalence; serum neutralization test is suitable for the non-infection certificate and clinical diagnosis of individual animals before moving
Bed cases, monitoring the prevalence of infection and evaluation of the immune effect:
2 Normative references
The following documents are indispensable for the application of this document: For dated reference documents, only the dated version applies to this article
Pieces: For undated references, the latest version (including all amendments) applies to this document:
GB/T 6682 Analytical laboratory water specifications and test methods
GB 19489 Laboratory Biosafety General Requirements
NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples
3 Abbreviations
The following abbreviations apply to this document:
CPE: Cytopathiceffect
DNA: Deoxyribonucleic acid (Deoxyribonucleicacid)
EDTA: EthyleneDiamineTetraacetic Acid (EthyleneDiamineTetraacetic Acid)
GMEM: Growth nutrient requirement medium (Glasgow'sModifiedEagle'sMedium)
LSD: bovine nodular skin disease (Lumpyskindisease)
LSDV: Bovine Nodular Skin Disease Virus (Lumpyskindiseasevirus)
LTc: Lambtestiscel
ORF: Open reading frame (Openreadingframe)
PBS: Phosphate buffered saline (Phosphatebufferedsaline)
PCR: Polymerase chain reaction (Polymerasechain reaction)
TCID50: half of tissue culture infection (Mediantissuecultureinfectivedose)
Vero: African green monkey kidney cells (Africangreenmonkeykidneycel)
VN: Virus neutralisation test (Virusneutralisationtest)
4 Clinical diagnosis
4:1 Susceptible animals
All kinds of cattle, including yellow cattle, dairy cows, Asian buffalo, zebu, yak are susceptible, sheep, goats and wild animals giraffes, black spots
Antelope and antelope can be infected artificially:
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