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GB/T 36816-2018: Detection and identification of Potato virus
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Detection and identification of Potato virus
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GB/T 36816-2018
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Basic data | Standard ID | GB/T 36816-2018 (GB/T36816-2018) | | Description (Translated English) | Detection and identification of Potato virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 22,283 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36816-2018: Detection and identification of Potato virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Potato Y virus quarantine and identification method)
ICS 65.020.01
B16
National Standards of People's Republic of China
Potato Y virus quarantine and identification method
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The organization is not responsible for identifying these patents.
This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This standard was drafted. Heilongjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhejiang University, China Academy of Inspection and Quarantine,
Institute of Virus-free Seedlings, Heilongjiang Academy of Agricultural Sciences.
The main drafters of this standard. Liu Hongyi, Liu Zhongmei, Liang Wusheng, Zhang Yongjiang, Zhang Xingzhe, Wei Meisheng, Li Guifen, Yuan Jianjiang, Fan Guoquan,
Feng Liping and Yang Liqun.
Potato Y virus quarantine and identification method
1 Scope
This standard specifies the quarantine and identification methods for potato virus Y.
This standard applies to the quarantine and identification of potato plants, propagation materials and other host plants that may have potato virus Y.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 7331 Potato seed potato quarantine regulations
SN/T 1840 plant virus immunoelectron microscopy detection method
SN/T 2122 Quarantine sampling method for inbound and outbound plants and plant products
3 Potato Y virus basic information
Chinese name. Potato Y virus
Scientific name. PotatovirusY
Abbreviation. PVY
Synonym. Potatoseveremosaicvirus, Potatoacropetalnecrosisvirus, Solanumvirus2, Tobaccovein-
Bandingvirus,Potatoleafdropstreakvirus,Tobaccoveinalnecrosisvirus,Potatovirus20
Classification status. Potyviridae, Potyvirus.
Transmission route. vegetative propagation materials such as potato seed potatoes are transmitted, mechanical vaccination can transmit drugs, and various mites spread the disease in a non-persistent manner.
poison. Long-distance transmission is accomplished through the trade of asexual propagation materials such as seed potatoes. According to the data, the potato seed can transmit the virus, but in the raw
There is little significance in production; no reports have been reported on the seeds of other Solanaceae crops.
See Appendix A for additional information on potato virus Y.
4 Principle of the method
The genomic characteristics, immunogenicity and symptom characteristics of the PVY are the basis for the quarantine and identification of the virus. According to PVY
And the genomic features of the main strains were established by reverse transcription-PCR (RT-PCR) and real-time fluorescent reverse transcription-PCR (Real-time RT-PCR) detection.
Method; based on the immunogenicity of PVY and major strains, an enzyme-linked immunosorbent assay (ELISA) method and an immunochromatographic strip tester were established.
Method and immunoelectron microscopy; based on PVY and the characteristic characteristics of major strains on different host plants, the identification of host diagnosis method;
A pyrophosphate sequencing method was used to identify strain groups based on the genome sequence of PVY. Through the effective combination of these methods, determine whether the sample carries
PVY and major strains.
5 Instruments, equipment and reagents
5.1 Instrumentation
Microplate reader, PCR instrument, real-time fluorescence PCR instrument, electrophoresis system, biological safety cabinet, small centrifuge, desktop refrigerated centrifuge, water bath,
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