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GB/T 36806-2018: Detection of Sugarcane bacilliform virus by real-time PCR
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Detection of Sugarcane bacilliform virus by real-time PCR
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GB/T 36806-2018
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Basic data | Standard ID | GB/T 36806-2018 (GB/T36806-2018) | | Description (Translated English) | Detection of Sugarcane bacilliform virus by real-time PCR | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 10,120 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36806-2018: Detection of Sugarcane bacilliform virus by real-time PCR---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of Sugarcane bacilliform virus by real-time PCR
ICS 65.020.01
B16
National Standards of People's Republic of China
Sugarcane baculovirus real-time fluorescence
PCR detection method
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This standard was drafted. National Sugarcane Engineering Technology Research Center of Fujian Agriculture and Forestry University, and quality supervision and inspection of sugarcane and products of the Ministry of Agriculture.
Center, Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Province.
The main drafters of this standard. Gao Sanji, Sun Shengren, Wu Xiaobin, Huang Meiting, Fu Huaying, Wang Jinda, Zhang Huili, Chen Rukai.
Sugarcane baculovirus real-time fluorescence
PCR detection method
1 Scope
This standard specifies sugarcane rod-shaped IM virus (SugarcanebacilliformIMvirus, SCBIMV) and sugarcane rod-shaped MO disease
Instruments and equipment, reagents and materials for the real-time fluorescent PCR detection method of Sugarcanebacilliform MOvirus (SCBMOV)
Sample collection and pre-processing, detection, and judgment and representation of results.
This standard applies to the rapid detection and diagnosis of living host plants that may have SCBIMV and SCBMOV viruses.
2 Abbreviations
The following abbreviations apply to this document.
CTAB. cetyltrimethylammonium bromide
Ct value. the number of cycles experienced by the fluorescent signal in each reaction tube reaching the set threshold in a real-time fluorescent PCR reaction (cycle
Threshold)
DNase. deoxyribonuclease
EDTA-Na2. disodium edetatedisodium
FAM. 6-carboxy-fluorescein
PCR. polymerase chain reaction (polymerase chain reaction)
RNase. ribonuclease
RT/RNaseH. reverse transcriptase/RNaseH
SCBIMV. SugarcanebacilliformIMvirus (SugarcanebacilliformIMvirus)
SCBMOV. Sugarcane bacillus MO virus (SugarcanebacilliformMOvirus)
SCBV. Sugarcane baculovirus (Sugarcanebacilliformvirus)
TAMRA. 6-carboxytetramethylrhodamine
Tris-HCl. Tris(hydroxymethyl)aminomethanehydrochloride]
3 Sugarcane baculovirus basic information
The sugarcane baculovirus belongs to the genus of the genus Caulimiviridae (Badnavirus).
It is called Sugarcanebacilliformvirus, abbreviated as SCBV. The International Classification of Viruses (ICTV) will be SCBV in.2012
Two isolates were officially designated as Sugarcane bacilliform IMvirus (SCBIMV) and cane stalked MO disease
Two different species of poison (Sugarcanebacilliform MOvirus, SCBMOV). See Appendix A for additional information on sugarcane baculovirus.
4 Principle of the method
Based on the alignment of the sugarcane baculovirus genome sequence, a pair of specific ones conserved only between the RT/RNaseH genes of the virus was designed.
Sex primers and a specific fluorescent double-tagged TaqMan probe. Fluorescence of TaqMan probes in real-time fluorescent PCR amplification reactions
The signal intensity is proportional to the increase of the PCR product of the target sequence, and the fluorescence signal value of the PCR amplification process is collected, ie
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