|
US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 36810-2018: Detection and identification of Fusarium oxysporum (Schlecht.) f. sp - Fragariae Winks & Williams
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36810-2018 | English | 279 |
Add to Cart
|
3 days [Need to translate]
|
Detection and identification of Fusarium oxysporum (Schlecht.) f. sp - Fragariae Winks & Williams
| Valid |
GB/T 36810-2018
|
PDF similar to GB/T 36810-2018
Basic data | Standard ID | GB/T 36810-2018 (GB/T36810-2018) | | Description (Translated English) | Detection and identification of Fusarium oxysporum (Schlecht.) f. sp - Fragariae Winks & Williams | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,169 | | Date of Issue | 2018-09-17 | | Date of Implementation | 2019-04-01 | | Regulation (derived from) | National Standard Announcement No. 11 of 2018 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36810-2018: Detection and identification of Fusarium oxysporum (Schlecht.) f. sp - Fragariae Winks & Williams
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Fusarium oxysporum (Schlecht.) f.sp.Fragariae Winks
ICS 65.020.01
B16
National Standards of People's Republic of China
Method for quarantine and identification of strawberry wilt pathogen
DetectionandidentificationofFusariumoxysporum (Schlecht.)f.sp.
FragariaeWinks
Published on.2018-09-17
Implementation of.2019-04-01
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Technical Committee for Phytosanitary Standardization (SAC/TC271).
This standard was drafted. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Liu Yueting, Luo Jiafeng, Niu Chunjing, Cui Tiejun, Liao Fang.
Method for quarantine and identification of strawberry wilt pathogen
1 Scope
This standard specifies the quarantine and identification methods for strawberry wilt pathogens.
This standard applies to the quarantine and identification of strawberry wilt pathogens in strawberry seedlings and their carrying soil.
2 Basic information on strawberry wilt pathogen
Chinese name. Strawberry Fusarium
Scientific name. Fusariumoxysporum (Schlecht.) f.sp.fragariaeWinks
English name. fusariumwiltofstrawberry
Classification status. Fungus, Fusarium, Ascomycota, Pezizomycotina,
Sordariomycetes, Hypocreales, Nectriaceae, Fusarium.
Route of transmission. The germs rely on the seedlings and soil for long-distance transmission.
See Appendix A for additional information on strawberry Fusarium.
3 Principle of the method
Symptoms, isolation and culture traits, morphological characteristics and PCR-specific amplified fragments of pathogenic bacteria as quarantine for strawberry wilt
Determine the basis.
4 instruments and main reagents
4.1 Apparatus
Microscope, ultra-clean workbench, biological incubator, electronic balance, autoclave, regular refrigerator (-20 °C). PCR amplification instrument, electrophoresis
Instrument, gel imager, high speed refrigerated centrifuge, constant temperature water bath.
Petri dishes, flasks, tweezers, surgical scissors, scalpels, inoculation needles, pipettes, alcohol lamps.
4.2 Primary reagents
Ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), Tris-HCl, isoamyl alcohol, chloroform, absolute ethanol, 70% B
Molecular biological reagents such as alcohol, potassium chloride, magnesium chloride, proteinase K, primers FofraF/FofraR, Taq polymerase. Or use plant tissue and
The fungal genome extraction kit extracts DNA. Sucrose, glucose, agar, penicillin, streptomycin, 0.5% sodium hypochlorite (NaClO).
See Appendix B for the preparation of potato sucrose medium (PSA) and potato dextrose medium (PDA).
5 Identification of pathogens
5.1 symptom check
Take the plant to be observed, observe whether the plant has poor growth, short stupidity and rot, and whether the lower leaves become purplish red, whether the heart leaves
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 36810-2018_English be delivered?Answer: Upon your order, we will start to translate GB/T 36810-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 36810-2018_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 36810-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|