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GB/T 35808-2018: Method for analysis of forestry biomass -- Determination of cellulase activity
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| GB/T 35808-2018 | English | 119 |
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Method for analysis of forestry biomass -- Determination of cellulase activity
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GB/T 35808-2018
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Basic data | Standard ID | GB/T 35808-2018 (GB/T35808-2018) | | Description (Translated English) | Method for analysis of forestry biomass -- Determination of cellulase activity | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B60 | | Classification of International Standard | 79.010 | | Word Count Estimation | 6,646 | | Date of Issue | 2018-02-06 | | Date of Implementation | 2018-09-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 35808-2018: Method for analysis of forestry biomass -- Determination of cellulase activity---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for analysis of forestry biomass - Determination of cellulase activity
ICS 79.010
B60
National Standards of People's Republic of China
Forestry biomass materials analysis
Cellulase activity assay
2018-02-06 released
2018-09-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the State Forestry Administration.
This standard by the National Forestry biomass materials Standardization Technical Committee (SAC/TC416) centralized.
This standard was drafted. Beijing Forestry University, Xiamen Standard & Poor's standardized services Limited.
The main drafters of this standard. Zhao Hongfei, Zhang Berlin, Chang Jianmin, Zhou Fang, Ren Xueyue, Xu Jinfei.
Forestry biomass materials analysis
Cellulase activity assay
1 Scope
This standard specifies the terms and definitions, principles, reagents and materials, apparatus and instruments used in the determination of cellulase activity under given conditions
And equipment, standard curve drawing, sample preparation, measurement procedures, calculation of results, repeatability requirements, test reports.
This standard applies to the determination of cellulase activity in forestry biomass materials analysis.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analysis of experimental water specifications and test methods
GB/T 30366-2013 Biomass terms
3 Terms and definitions
GB/T 30366-2013 defined and the following terms and definitions apply to this document. For ease of use, the following list is repeated
GB/T 30366-2013 some of the terms and definitions.
3.1
Forestry biomass forestrybiomass
Biomass produced during forestry production and processing. Mainly include forest products (such as wood, bamboo, rattan, etc.), forestry residues (such as branches
Ah, sawdust, sawdust, tops, skin and truncated heads, harvesting residues and processing residues such as nutshell and kernels, papermaking waste, and discarded wood)
Energy forest.
[GB/T 30366-2013, Definition 2.1.3]
3.2
Cellulase activity unit celulaseactivityunit
The phase was released from the sodium carboxymethyl cellulose (CMC-Na) solution at a concentration of 5 g/L per minute at 37 ° C, pH 5.5
When the amount of reducing sugar in 1μmol glucose is one enzyme activity unit (U).
4 principle
Cellulase at a certain temperature and pH conditions, the hydrolysis of carboxymethyl cellulose produced by cellobiose, glucose and other reducing sugars can be alkaline
Under the conditions of 3,5-dinitrosalicylic acid (DNS) reduction, the generation of brown-red amino compounds, maximum absorption at 540nm wavelength,
The amount of reducing sugar produced by enzymolysis within a certain range is proportional to the absorbance of the reaction solution, and the amount of reducing sugar produced can be measured by a colorimetric method
Cellulase activity was calculated.
5 Reagents and materials
5.1 All reagents used in this standard unless otherwise specified, are of analytical grade, water are in line with the provisions of GB/T 6682 secondary water.
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