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GB/T 35806-2018: Animal influenza detection -- Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9
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| GB/T 35806-2018 | English | 199 |
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Animal influenza detection -- Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9
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GB/T 35806-2018
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Basic data | Standard ID | GB/T 35806-2018 (GB/T35806-2018) | | Description (Translated English) | Animal influenza detection -- Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9 | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,174 | | Date of Issue | 2018-02-06 | | Date of Implementation | 2018-09-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 35806-2018: Animal influenza detection -- Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9
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Animal influenza detection-Protocol of duplex real-time RT-PCR for influenza virus subtypes H7 and N9
ICS 11.220
B41
National Standards of People's Republic of China
Animal Flu Detects H7N9 Influenza Virus
Dual fluorescent RT-PCR detection method
Published on.2018-02-06
2018-09-01 Implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by the Ministry of Agriculture.
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181).
This standard was drafted by. Beijing Entry-Exit Inspection and Quarantine Bureau Inspection and Quarantine Technology Center, China Entry-Exit Inspection and Quarantine Association, China Agriculture
Harbin Institute of Veterinary Science, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Pu Jing, Gao Zhiqiang, Wang Lin, Qiao Caixia, Zhang Xiquan, Zhou Qi, Zhang Hexiao, Zhang Wei, Yin Hao, Bai Yaxi, Liu Yanhua,
Liu Huan, Wang Xiurong, Bao Hongmei, Han Xueqing, and Wang Huiyan.
Animal Flu Detects H7N9 Influenza Virus
Dual fluorescent RT-PCR detection method
1 Scope
This standard specifies TaqMan dual-fluorescence RT-PCR procedures for the simultaneous detection of influenza A nucleic acid in H7 and N9 subtype animals.
method.
This standard applies to the rapid detection and screening of animal influenza virus H7 and N9 subtypes.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
GB/T 19438.1-2004 Avian influenza virus universal fluorescence RT-PCR detection method
GB 19489 General Requirements for Laboratory Biosafety
3 Abbreviations
The following abbreviations apply to this document.
Ct (or Cp) value. the number of cycles experienced by the amount of fluorescence signal in each reaction tube reaching the set threshold (ThresholdCycle, or
CrossingPoint)
DEPC. Diethypyrocarbonate
FAM. Carboxyfluorescein
HA. Hemagglutinin
HEX. Hexachlorofluorescein
MGB . Minor GrooveBinder
NA. Neuraminidase
TAMRA. Carboxytetramethylrhodamine
Fluorescence RT-PCR. Real-time RT-PCR
4 Reagents and materials
4.1 Reagents
4.1.1 General. Unless otherwise stated, all reagents used are of analytical grade. All liquid reagents used shall be packed in RNase-free containers.
4.1.2 TRIzol. Store at 2°C to 25°C.
4.1.3 Chloroform. Precooling at 2°C to 8°C.
4.1.4 Isopropyl alcohol. Precooled at -20°C.
4.1.5 DEPC water. See A.1 for recipes.
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