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GB/T 33409-2016: Determination of the activity of beta-galactosidase -- Spectrophotometric method
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 33409-2016 | English | 119 |
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Determination of the activity of beta-galactosidase -- Spectrophotometric method
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GB/T 33409-2016
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Basic data | Standard ID | GB/T 33409-2016 (GB/T33409-2016) | | Description (Translated English) | Determination of the activity of beta-galactosidase -- Spectrophotometric method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G66 | | Classification of International Standard | 71.040.30 | | Word Count Estimation | 6,676 | | Date of Issue | 2016-12-30 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | National Standard Notice No.27 of 2016 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 33409-2016: Determination of the activity of beta-galactosidase -- Spectrophotometric method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of beta-galactosidase - Spectrophotometric method
ICS 71.040.30
G66
National Standards of People's Republic of China
Determination of β - galactosidase activity by spectrophotometry
2016-12-30 release
2017-07-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard by the National Biochemical Testing Standardization Technical Committee (SAC/TC387) proposed and centralized.
The drafting of this standard. Shenzhen Institute of Measurement and Quality Inspection, China Testing Technology Research Institute.
The main drafters of this standard. Zhang Shiwei, Zhou Lihua, Lai Xintian, Chen Shuijian, Tang Dong, Hong Xiaoming, Wang Zhenni, Yang Guowu.
Determination of β - galactosidase activity by spectrophotometry
1 Scope
This standard specifies Kluyveromyces lactis, Kluyveromyces
fragilis, Kluyveromyces marxianus, Saccharomyces fragilis, large intestine
Escherichia coli, Aspergillus melalea, Aspergillus niger,
Detection of glycosidase activity.
This standard is applicable to the determination of β-galactosidase activity in biochemical reagents and industrial enzymes.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 terms and definitions
The following terms and definitions apply to this document.
3.1
β-galactosidase activity unit beta-galactosidaseactivityunit
The amount of enzyme that catalyzes the conversion of one micromolar o-nitrobenzene-β-D-galactoside per minute under specified reaction conditions.
4 principle
β-galactosidase can rapidly catalyze o-nitrobenzene-β-D-galactoside to produce o-nitrophenol and galactose by changing the absorbance value
The amount of o-nitrophenol was calculated to calculate the enzyme activity.
5 Instruments and equipment
5.1 pH meter. accurate to 0.01pH.
5.2 Analysis of the balance. the amount of 0.0001g.
5.3 UV-Vis spectrophotometer. Wavelength Accuracy ± 1nm, absorbance value accurate to 0.001.
5.4 1cm quartz cuvette.
5.5 constant temperature water bath. temperature control accuracy ± 0.5 ℃.
6 reagent
Unless otherwise specified, the reagents used in this method are of analytical grade and water is the tertiary water specified in GB/T 6882.
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