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GB/T 33410-2016: Determination of the activity of protease K in biochemical reagents
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| GB/T 33410-2016 | English | 119 |
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Determination of the activity of protease K in biochemical reagents
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GB/T 33410-2016
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Basic data | Standard ID | GB/T 33410-2016 (GB/T33410-2016) | | Description (Translated English) | Determination of the activity of protease K in biochemical reagents | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G66 | | Classification of International Standard | 71.040.30 | | Word Count Estimation | 6,642 | | Date of Issue | 2016-12-30 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | National Standard Notice No.27 of 2016 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 33410-2016: Determination of the activity of protease K in biochemical reagents---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of protease K in biochemical reagents
ICS 71.040.30
G66
National Standards of People's Republic of China
Determination of Protease K Activity in Biochemical Reagents
2016-12-30 release
2017-07-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard by the National Biochemical Testing Standardization Technical Committee (SAC/TC387) proposed and centralized.
The drafting of this standard. Shenzhen Institute of Measurement and Quality Inspection, China Testing Technology Research Institute, China Institute of Standardization.
The main drafters of this standard. Lin Lin, Zhou Lihua, Lai Xintian, Lan Quanxue, Ma Aijin, Chen Guobei, Li Xixi, Pan Lanfang, Yang Guowu.
Determination of Protease K Activity in Biochemical Reagents
1 Scope
This standard specifies the biochemical reagents in the protease K activity detection method.
This standard applies to the determination of protease K activity used in molecular biology experiments.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 terms and definitions
The following terms and definitions apply to this document.
3.1
Protease K activity unit proteaseKactivityunit
At 55 ℃, pH8.0, 1min hydrolysis of casein to produce 1μgL-tyrosine as an enzyme activity unit.
Note. The activity units are U/mg, U/g or U/mL.
4 principle
Protease K is a cleavable serine protease that catalyzes the hydrolysis of alpha-casein containing more serine sites.
In this method, α-casein is used as the reaction substrate, and hydrolyzed to L-tyrosine by protease K, and the absorbance value is measured at 275 nm. again
The linear regression equation was obtained by measuring the absorbance values of L-tyrosine standard solution at 275nm, and the standard curve was constructed.
Outside the standard method of quantitative. Calculate the enzymatic activity of protease K.
5 Instruments and equipment
5.1 constant temperature water bath. temperature control accuracy of ± 0.2 ℃.
5.2 pH meter. accurate to 0.01pH.
5.3 UV - Visible spectrophotometer. absorbance value accurate to 0.001.
5.4 1cm quartz cuvette.
5.5 electronic balance. sense of 0.0001g.
6 reagent
Unless otherwise specified, the reagents used in this method are of analytical grade and water is the tertiary water specified in GB/T 6882.
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