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GB/T 32948-2016: Detection of coproantigen of canidae infected Echinococcus granulosus (e.g.) by sandwich enzyme-linked immunosorbent assay
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 32948-2016 | English | 219 |
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Detection of coproantigen of canidae infected Echinococcus granulosus (e.g.) by sandwich enzyme-linked immunosorbent assay
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GB/T 32948-2016
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Basic data | Standard ID | GB/T 32948-2016 (GB/T32948-2016) | | Description (Translated English) | Detection of coproantigen of canidae infected Echinococcus granulosus (e.g.) by sandwich enzyme-linked immunosorbent assay | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 11,126 | | Date of Issue | 2016-08-29 | | Date of Implementation | 2017-03-01 | | Regulation (derived from) | National Standard Announcement 2016 No.14 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 32948-2016: Detection of coproantigen of canidae infected Echinococcus granulosus (e.g.) by sandwich enzyme-linked immunosorbent assay
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Detection of coproantigen of canidae infected Echinococcus granulosus (Eg) by sandwich enzyme-linked immunosorbent assay
ICS 11.220
B41
National Standards of People's Republic of China
Canine Infection of Echinococcus granulosus
Antigenic antibody sandwich enzyme - linked immunosorbent assay
Test detection technology
Detection ofcoproantigenofcanidaeinfected
Echinococcusgranulosus (Eg) bysandwichenzyme-linkedimmunosorbentassay
2016-08-29 released
2017-03-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by the Ministry of Agriculture of the People's Republic of China.
This standard by the National Animal Epidemic Standardization Technical Committee (SAC/TC181) centralized.
The drafting of this standard. Xinjiang Uygur Autonomous Region Institute of Veterinary Science, Institute of Animal Health and Epidemiology.
The drafters of this standard. Zhang Chongzhi, Zhang Wenbao, Zhang Xu, Mi Xiaoyun, Gu Nouer Tulson, Shi Baoxin, Fan Weixing,
Zhao Li, Wu Jian, Wang Jincheng.
Canine Infection of Echinococcus granulosus
Antigenic antibody sandwich enzyme - linked immunosorbent assay
Test detection technology
1 Scope
This standard specifies the sandwich enzyme-linked immunosorbent assay (SandwichELISA)
Claim.
This standard is applicable to the diagnosis, quarantine, efficacy assessment and related epidemiology of terminal dendritic echinococcosis infection (canine)
survey.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB 19489 General requirements for laboratory biosecurity
3 terms and definitions
The following terms and definitions apply to this document.
3.1
Echinococcus granulosus fecalantigenofEchinococcusgranulosus
Echinococcus granulosus parasitic in the dog's gut, with the feces together with the discharge of insects, metabolites and fall off the pregnancy.
3.2
Antibody sandwich antibodysandwich
Polyclonal antibodies and antigen immune binding, antigen and monoclonal antibodies with specific binding, the formation of antibody - antigen - antibody "sandwich"
Type complexes.
3.3
Echinococcus granulosus Eggs
The surface antigen (EgsfAg) obtained by the action of 1% Triton × 100 in Echinococcus granulosus adults.
3.4
Rabbit anti-EgsfAg immunoglobulin Gtherabbit'simmunoglobulinGagainstEgsfAg
Purified anti-EgsfAg polyclonal rabbit antibody IgG (protein content of 11.12mg/mL, antibody titer of 1.160000 or more).
3.5
Anti-EgsfAg mouse monoclonal antibody mousemonoclonalantibodyagainstEgsfAg
Anti-EgsfAg mouse monoclonal antibody (protein content of 14.4mg/mL, antibody titer of 1. 800000 or more).
4 abbreviations
The following abbreviations apply to this document.
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