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GB/T 31581-2015: Code of practice on production of bovine frozen sexed-semen
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 31581-2015 | English | 189 |
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Code of practice on production of bovine frozen sexed-semen
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GB/T 31581-2015
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Basic data | Standard ID | GB/T 31581-2015 (GB/T31581-2015) | | Description (Translated English) | Code of practice on production of bovine frozen sexed-semen | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B43 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 9,928 | | Date of Issue | 2015-05-15 | | Date of Implementation | 2015-10-01 | | Quoted Standard | GB 4143; GB/T 5458; GB/T 31582; NY/T 1234 | | Regulation (derived from) | National Standard Announcement 2015 No. 15 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This Standard specifies the bovine sexed frozen semen production of equipment for cleaning and disinfection, dilution preparation, semen, semen processing, sperm separation, freezing, thawing, testing, packaging, storage and transport. This Standard applies to the use of flow cytometry separation technology to produce frozen bovine sexed semen. | GB/T 31581-2015: Code of practice on production of bovine frozen sexed-semen---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Code of pratice on production of bovine frozen sexed-semen
ICS 65.020.30
B43
National Standards of People's Republic of China
Bovine sexed frozen semen production and technological processes
Issued on. 2015-05-15
2015-10-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Standardization Technical Committee on Animal Husbandry (SAC/TC274) centralized.
This standard was drafted. the Ministry of Agriculture frozen bovine semen Quality Supervision and Testing Center (Beijing), 内蒙古赛科 star breeding biotechnology stocks
Parts Limited, Beijing Dairy Cattle Center, China Agricultural University, National Animal Husbandry, Ministry of Agriculture, frozen bovine semen Quality Supervision and Testing Center
(Nanjing).
The main drafters. Zhang Xiaoxia, and Xi, Zhou Wenzhong, Liu Hailiang, Sun Feizhou, Zhang Shengli, Luhan Xi, Yang Qingfeng, Wu Yubo,
Qian Jin Song, Zhang Haitao, Liu, Zhao, Wang Jianguo, Hu Shu Xiang, Zhang Yong, Hu Zhigang.
Bovine sexed frozen semen production and technological processes
1 Scope
This standard specifies the bovine sexed frozen semen production equipment cleaning and disinfection, diluted liquid preparation, semen, semen processing, sperm separation, cold
Frozen, thawed, testing, packaging, storage and transport.
This standard applies to the use of flow cytometry separation technology to produce bovine sexed frozen semen.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 4143 frozen bovine semen
GB/T 5458 liquid nitrogen container
GB/T 31582 bovine sexed frozen semen
NY/T 1234 frozen bovine semen production and technological processes
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Sperm separation spermsorting
Based on differences in X and Y sperm sperm DNA content by flow separation method X sperm and Y sperm separated.
3.2
Sexed frozen semen frozensexed-semen
After separation of sperm-rich X or Y sperm after cryopreservation in liquid nitrogen for long term storage of semen.
4 semen supplies cleaning and disinfecting
Accordance with the provisions NY/T 1234's.
5 solution preparation and sterilization
5.1 solution preparation
5.1.1 dilution
Diluent preparation see Appendix A, A.1.
5.1.2 Separator buffer Tris-sheath
Separator buffer Tris-sheath formulation see A.2.
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