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GB/T 31408-2015: Determination of phenacetin in hair dye -- Liquid chromatography method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 31408-2015 | English | 149 |
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Determination of phenacetin in hair dye -- Liquid chromatography method
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GB/T 31408-2015
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Basic data | Standard ID | GB/T 31408-2015 (GB/T31408-2015) | | Description (Translated English) | Determination of phenacetin in hair dye -- Liquid chromatography method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 7,767 | | Date of Issue | 2015-05-15 | | Date of Implementation | 2015-09-30 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standard Announcement 2015 No. 15 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This Standard specifies the method of hair dye liquid chromatography phenacetin of Central Africa. This Standard applies to the determination of hair dye phenacetin of Central Africa. | GB/T 31408-2015: Determination of phenacetin in hair dye -- Liquid chromatography method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of phenacetin in hair dye. Liquid chromatography method
ICS 71.100.70
Y42
National Standards of People's Republic of China
Determination of hair dye Central Africa phenacetin liquid chromatography
Issued on. 2015-05-15
2015-09-30 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Quality Supervision key product testing method Standardization Technical Committee (SAC/TC374) and focal points.
This standard was drafted. Guangzhou Quality Supervision and Testing Institute, Zhaoqing Di Choi Chemical Co., Ltd., Guangzhou Baiyun Linkman fine
Factories, National Quality Supervision and Inspection Center Cosmetics (Guangzhou), Fujian Province, product quality inspection Institute, Hebei Province, Food Quality Supervision and Inspection Institute
Hospital studies, Quality Inspection Institute of Zhejiang, Guangdong Sheng Weina Fine Chemical Co., Ltd.
Drafters of this standard. Xie Jian, Chen Liwei, Wu Yu Luan Party of China, Luo Haiying, Huang Yufeng, Guo Changhong, Han Wanqing, Xian Yanping, Deming, Zhang Yan,
He Qiaosang, Wang Yi, Wang Bin, Wang Chen school, JiangFu whole, Zhao Jinsong.
Determination of hair dye Central Africa phenacetin liquid chromatography
1 Scope
This standard specifies the liquid chromatographic method of hair dye Central Africa phenacetin.
This standard applies to the determination of hair dye phenacetin of Central Africa.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
Sample of phenacetin by aqueous methanol extract, measured by HPLC, external standard.
4 instruments and materials
4.1 Water. GB/T 6682 provided a water.
4.2 Methanol. chromatographically pure.
4.3 hexane. AR.
4.4 acetonitrile. chromatography.
4.5 phenacetin standard substance. purity ≥99%; CAS. 62-44-2.
4.6 70% aqueous solution of methanol. water = V 73 V methanol.
4.7 standard stock solution (5.0mg/mL). Weigh accurately standard substance (4.5) 50.0mg, 70% aqueous methanol (4.6) was dissolved and set the volume
To 10mL.
4.8 standard working solution. Pipette appropriate amount of stock standard solution (4.7) with 70% aqueous methanol (4.6) stepwise diluted concentrations were
0μg/mL, 0.5μg/mL, 2.0μg/mL, 10.0μg/mL, 20.0μg/mL, 50.0μg/mL series of standard working solution.
5. Apparatus
5.1 HPLC or UHPLC. with diode array detector.
5.2 Analytical balance. accuracy of 0.1mg.
5.3 Vortex.
5.4 ultrasonic cleaning. ultrasonic power 250W.
5.5 centrifuge. speed of not less than 2500r/min.
5.6 Membrane. nylon membrane, pore size 0.22μm.
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