|
US$169.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 30957-2014: Determination of ochratoxin A in feeds -- High performance liquid chromatography with immunoaffinity column clean-up
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 30957-2014 | English | 169 |
Add to Cart
|
3 days [Need to translate]
|
Determination of ochratoxin A in feeds -- High performance liquid chromatography with immunoaffinity column clean-up
| Valid |
GB/T 30957-2014
|
PDF similar to GB/T 30957-2014
Basic data | Standard ID | GB/T 30957-2014 (GB/T30957-2014) | | Description (Translated English) | Determination of ochratoxin A in feeds -- High performance liquid chromatography with immunoaffinity column clean-up | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 7,774 | | Date of Issue | 7/8/2014 | | Date of Implementation | 1/10/2015 | | Quoted Standard | GB/T 6682; GB/T 14699.1; GB/T 20195 | | Regulation (derived from) | 2014 National Standards Bulletin No. 18 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the feed of ochratoxin A immunoaffinity column clean - high performance liquid chromatographic method. This standard applies to feed materials, compound feed, concentrated feed, feed concentrate supplement Determination of ochratox | GB/T 30957-2014: Determination of ochratoxin A in feeds -- High performance liquid chromatography with immunoaffinity column clean-up
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of ochratoxin A in feeds. High performance liquid chromatography with immunoaffinity column clean-up
ICS 65.120
B46
National Standards of People's Republic of China
Feed Determination of ochratoxin A in
Immunoaffinity column clean - High Performance Liquid Chromatography
Issued on. 2014-07-08
2015-01-10 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Feed Industry Standardization Technical Committee (SAC/TC76) centralized.
This standard by the Ministry of Agriculture Feed Quality Supervision and Testing Center (Nanchang), Chinese Academy of Agricultural Sciences Agricultural Quality Standards and Testing Technology Institute
The study [National Feed Quality Supervision and Inspection Center (Beijing)], the quality of agricultural Standards and Testing Technology Research Institute, Shanghai Academy of Agricultural Sciences is responsible
Drafted.
The main drafters of this standard. Rao Hui, Wen Hong, Zhou Hua Jiao, Fu Jinhua, Xu Tian put, Rao Zhenghua, Yinteng Gui, Yang Linfen, Xing Lei, Zhao Weina,
Zhao Zhihui.
Feed Determination of ochratoxin A in
Immunoaffinity column clean - High Performance Liquid Chromatography
1 Scope
This standard specifies the feed of ochratoxin A immunoaffinity column clean - high performance liquid chromatographic method.
This standard applies to feed materials, compound feed, concentrated feed, feed concentrate supplement Determination of ochratoxin A in.
The standard limit of quantitation was 5.0μg/kg.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 14699.1 feed samples
GB/T 20195 Animal feed sample preparation
Principle 3
After the sample methanol - water extract, the extract was filtered, diluted filtrate containing ochratoxin A through specific antibody immunoaffinity
Column chromatography, eluted with PBS buffer and washed with pure water, methanol eluate was measured by high performance liquid chromatography with fluorescence detector
Given ochratoxin A content of toxins.
4 Reagents
Unless otherwise specified, reagents were analytical reagent grade water comply with GB/T 6682 in two water.
4.1 Methanol. HPLC grade.
4.2 acetonitrile. HPLC grade.
4.3 sodium chloride.
4.4 dibasic sodium phosphate.
4.5 potassium dihydrogen phosphate.
4.6 potassium chloride.
4.7 hydrochloric acid.
4.8 sodium bicarbonate.
4.9 Tween -20 (Tween-20).
4.10 acetic acid.
4.11 washing buffer. 25g of sodium chloride (4.3), 5g of sodium bicarbonate (4.8) was dissolved in water, was added 0.1mL Tween-20 (4.9), water
Diluted to 1L.
4.12 PBS buffer solution. Weigh 8.0g of sodium chloride (4.3), 1.2g disodium hydrogen phosphate (4.4), 0.2g potassium dihydrogen phosphate (4.5), 0.2g chloride
Potassium (4.6) was dissolved in 900mL of water, adjusted with concentrated hydrochloric acid (4.7) pH value to 7.0, to volume with water to 1L.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 30957-2014_English be delivered?Answer: Upon your order, we will start to translate GB/T 30957-2014_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 30957-2014_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 30957-2014_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|