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Determination of chlorpropamide, tolbutamide and carbutamide 3 banned sulfonylureas in cosmetics
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GB/T 30936-2014
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Basic data | Standard ID | GB/T 30936-2014 (GB/T30936-2014) | | Description (Translated English) | Determination of chlorpropamide, tolbutamide and carbutamide 3 banned sulfonylureas in cosmetics | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 13,190 | | Date of Issue | 7/8/2014 | | Date of Implementation | 11/1/2014 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | 2014 National Standards Bulletin No. 18 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the cosmetics chlorpropamide, tolbutamide and carbutamide three kinds of substances sulfonylurea disabled liquid chromatography (HPLC) and liquid chromatography - mass spectrometry/mass spectrometry (LC-MS/MS)test methods. This sta |
GB/T 30936-2014: Determination of chlorpropamide, tolbutamide and carbutamide 3 banned sulfonylureas in cosmetics ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of chlorpropamide, tolbutamide and carbutamide 3 banned sulfonylureas in cosmetics
ICS 71.100.70
Y42
National Standards of People's Republic of China
Cosmetics chlorpropamide, tolbutamide and carbutamide
Determination of three kinds of substances sulfonylurea disabled
Determinationofchlorpropamide, tolbutamideandcarbutamide
Issued on. 2014-07-08
2014-11-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard proposed by China National Light Industry Council.
This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized.
This standard was drafted. Shandong Institute of Product Quality Supervision, Inspection and the State Environmental Protection Product Quality Supervision and Inspection Center, Shandong Freda
Biological Engineering Co., Ltd., Shanghai Daily Chemical Industry Institute (Fragrance Flavor and Cosmetic National Quality Supervision and Inspection Center).
The main drafters of this standard. Wang Jun, Zhou Lili, ACADAMIC LIBRARY Zhang Yan, Zhao Wengang, Hu Mei, Yang Suzhen, Li Hui, Shenzhong Lan, Wang Yanli,
Yu Wen Jiang, Yang Ying, Qian Yan.
Introduction
The standard test substance is banned substances of "Hygienic Standard for Cosmetics" provisions, not as a cosmetic raw material, component
Added to cosmetics. If technically unavoidable banned substances as impurities into cosmetics, the finished cosmetic products should follow the "Cosmetics
Health standards "General requirements for cosmetics, that is, under normal and reasonably foreseeable conditions of use, should not cause harm to human health.
China has yet to establish these limit values \u200b\u200bsubstances present standards, the determination of these substances in cosmetics only provide detection methods.
Cosmetics chlorpropamide, tolbutamide and carbutamide
Determination of three kinds of substances sulfonylurea disabled
1 Scope
This standard specifies performance liquid chromatography cosmetics chlorpropamide, tolbutamide and carbutamide three kinds of substances sulfonylurea disabled
(HPLC) and liquid chromatography - mass spectrometry/mass spectrometry (LC-MS/MS) measurement method.
This standard applies to creams, lotions, liquid cosmetics etc. chlorpropamide quantitative, tolbutamide and carbutamide measured in; liquid chromatography
Spectrum - mass spectrometry/mass spectrometry applied to cosmetics sulfonylureas substances qualitative confirmation.
Determination of the standard liquid chromatography chlorpropamide, tolbutamide, carbutamide detection limit of 20mg/kg, the limit of quantification of 60mg/kg; Liquid
Mass spectrometry/mass spectrometry chlorpropamide, tolbutamide, carbutamide detection limit of 0.025mg/kg, the limit of quantification of 0.080mg/kg - chromatography.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Method 3 first high performance liquid chromatography (HPLC)
3.1 Principle
Acid extracted with methanol, and the extract after centrifugation, were determined by HPLC. External standard.
3.2 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
3.2.1 Methanol. chromatographically pure.
3.2.2 formic acid.
3.2.3 formic acid methanol solution (1%). The exact amount of 1mL 99mL of methanol and formic acid, after mixing spare.
3.2.4 formic acid (0.2%). The exact amount of 0.2mL 99.8mL formic acid and water, mix and reserve.
3.2.5 Standard chlorpropamide, tolbutamide standards, carbutamide standard. not less than 98% purity, chlorpropamide, tolbutamide
Urea and ammonia tolbutamide CAS number, molecular formula, molecular mass and structural formula, see Appendix A.
3.2.6 Standard stock solution. Weigh accurately chlorpropamide, tolbutamide and carbutamide standard each 0.1g, accurate to 0.0001g, points
Do a 50mL beaker, add appropriate amount of methanol dissolved quantitatively transferred to 100mL solution colorimetric tube, diluted with methanol to the mark, mix, that
Have a concentration of 1000mg/L of standard stock solution. -18 ℃ cryopreservation.
3.2.7 mixed standard stock solution. Pipette the appropriate amount each standard stock solution (3.2.6), diluted with methanol. 1 ℃ ~ 4 ℃ cold storage.
3.2.8 microporous membrane. 0.2μm, organic phase.
3.3 instruments and equipment
3.3.1 High performance liquid chromatography (HPLC) instrument. with a UV detector or diode array detector.
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