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GB/T 30927-2014: Determination of 4 prohibited coloring agents including rhodamine B in cosmetics -- High performance liquid chromatography
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Determination of 4 prohibited coloring agents including rhodamine B in cosmetics -- High performance liquid chromatography
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GB/T 30927-2014
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Basic data | Standard ID | GB/T 30927-2014 (GB/T30927-2014) | | Description (Translated English) | Determination of 4 prohibited coloring agents including rhodamine B in cosmetics -- High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 12,175 | | Date of Issue | 7/8/2014 | | Date of Implementation | 11/1/2014 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | 2014 National Standards Bulletin No. 18 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the cosmetics Rhodamine B, Acid Yellow 36, Pigment Red 53: 1 and high performance liquid chromatographic method Pigment Orange 5, 4 kinds of banned colorant. | GB/T 30927-2014: Determination of 4 prohibited coloring agents including rhodamine B in cosmetics -- High performance liquid chromatography
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Determination of 4 prohibited coloring agents including rhodamine B in cosmetics.High performance liquid chromatography
ICS 71.100.70
Y42
National Standards of People's Republic of China
Cosmetics Rhodamine B and other four kinds of banned colorant
HPLC
Issued on. 2014-07-08
2014-11-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard proposed by China National Light Industry Council.
This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Shanghai Institute of Daily Chemical Industry.
The main drafters of this standard. Ma Qiang, Li Wentao, birch, Makuai Juan, Wang Ye, Meng double Lijing Rui Ding Lan TRANSACTIONS OF Li Qiong, Wu Xiaojian.
Introduction
This standard test substance is banned substances of "Hygienic Standard for Cosmetics" shall consist of not as a cosmetic raw material, component Tim
Added to cosmetics. If technically unavoidable banned substances as impurities into cosmetics, the finished cosmetic products shall comply with the "cosmetic Wei
Health standard "General requirements for cosmetics, that is, under normal and reasonably foreseeable conditions of use, should not cause harm to human health.
At present, China has not yet specified the substance of limited value, the present standards, the determination of these substances in cosmetics only provide detection methods.
Cosmetics Rhodamine B and other four kinds of banned colorant
HPLC
1 Scope
The standard provides a highly efficient liquid cosmetics Rhodamine B, Acid Yellow 36, Pigment Red Pigment Orange 5 53.1 and four kinds of banned colorant
Phase chromatographic method.
This standard applies to creams, loose powder, liquid, shampoo and lipstick Cosmetics Rhodamine B, Acid Yellow 36, Pigment Red 53.1 and pigments
Determination of four kinds of orange colorant disabled 5 and the like.
The standard detection and quantitation limits. Rhodamine B detection limit of 2mg/kg, the limit of quantification of 4mg/kg; loose powder category, liquid category and lip
Ointments sample detection limit Acid Yellow 36 is 4mg/kg, the limit of quantification for the 8mg/kg, Cream and shampoo samples in acid yellow class seized 26
The limit of 3mg/kg, the limit of quantification of 6mg/kg; loose powder category, liquid category and lipstick red pigment sample detection limit of 53.1
6mg/kg, the limit of quantification of 10mg/kg, Cream and shampoo samples Pigment Red 53.1 class detection limit of 4mg/kg, the limit of quantification
8mg/kg; detection limit of Pigment Orange 5 is 3mg/kg, the limit of quantification of 6mg/kg.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
By solvent extraction of the sample, after centrifugation, were determined by HPLC, external standard, liquid chromatography - mass spectrometry confirmed.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.1 acetonitrile. chromatography.
4.2 Methanol. chromatographically pure.
4.3 tetrahydrofuran. chromatography.
4.4 40% tetrabutyl ammonium hydroxide solution (volume fraction).
4.5 citric acid.
4.6 28% aqueous ammonia solution (volume fraction).
4.7 Standard. Rhodamine B and other four kinds of banned colorant standard Chinese name, English name, structural formula, CAS number, CI number, molecular formula,
Relative molecular mass See Appendix A, Table A.1, purity of not less than 95%.
4.8 standard stock solution. namely, accurately weighed amount of Rhodamine B, Acid Yellow 36 and Pigment Red 53.1 standard substance (accurate to 0.0001g),
Methanol (4.2) were prepared at a concentration of 1000μg/mL of the standard stock solution. Accurately weighed amount of reference material Pigment Orange 5 in B
Nitrile (4.1) formulated at a concentration of 200μg/mL standard stock solution. Each standard stock solution at 4 ℃ dark preservation, can be used for three months.
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