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GB/T 30390-2013: Determination of monosaccharide and disaccharide in oil seeds -- High-performance liquid chromatography
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| GB/T 30390-2013 | English | 169 |
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Determination of monosaccharide and disaccharide in oil seeds -- High-performance liquid chromatography
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GB/T 30390-2013
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Basic data | Standard ID | GB/T 30390-2013 (GB/T30390-2013) | | Description (Translated English) | Determination of monosaccharide and disaccharide in oil seeds -- High-performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B33 | | Classification of International Standard | 67.200.20 | | Word Count Estimation | 7,711 | | Quoted Standard | GB 5491; GB/T 6682; GB/T 14489.1 | | Regulation (derived from) | National Standards Bulletin 2013 No. 27 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the seed oil of fructose, glucose and sucrose content determined by HPLC. This standard applies to the determination of oil seeds in fructose, glucose and sucrose content in. The standard method detection limit: fructose, glucose, | GB/T 30390-2013: Determination of monosaccharide and disaccharide in oil seeds -- High-performance liquid chromatography
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Determination of monosaccharide and disaccharide in oil seeds. High-performance liquid chromatography
ICS 67.200.20
B33
National Standards of People's Republic of China
Determination of oil seeds fructose, glucose, sucrose content
High Performance Liquid Chromatography
Issued on. 2013-12-31
2014-06-22 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the People's Republic of China Ministry of Agriculture crop management division.
This standard was drafted. Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, the Ministry of Agriculture and oil products Quality Supervision, Inspection and Test Center.
The main drafters of this standard. Li Peiwu, Hu wind, Zhang, Ding Xiaoxia, Zhou Haiyan.
Determination of oil seeds fructose, glucose, sucrose content
High Performance Liquid Chromatography
1 Scope
This standard specifies the oil seeds fructose, glucose, sucrose content determined by HPLC.
This standard applies to the determination of oil seeds fructose, glucose, sucrose content in.
The standard method detection limit. fructose, glucose, sucrose are 2mg/g.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 5491 grain and oilseeds testing for sampling, and sample method
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 14489.1 Determination of moisture and volatile oil content
Principle 3
After precipitation samples extracted fat and protein filtrate by amino column separated by high performance liquid chromatography (with a refractive index detector
Or evaporative light scattering detector) measurement, external standard.
4 Reagents and materials
Unless otherwise indicated, the use of analytical reagent water as a secondary water GB/T 6682 regulations.
4.1 acetonitrile. chromatography.
4.2 petroleum ether. boiling range 30 ℃ ~ 60 ℃.
4.3 zinc acetate solution (1mol/L). Weigh 21.9g zinc acetate (C4H6O4Zn · 2H2O), add 3mL of glacial acetic acid, dissolved in water diluted
To 100mL.
4.4 potassium ferrocyanide solution (1mol/L). Weigh 10.6g of potassium ferrocyanide {K4 [Fe (CN) 6] · 3H2O}, dissolved in water and diluted to
100mL.
4.5 fructose, glucose, sucrose standard purity ≥99%.
4.6 sugar series stock standard solution (20mg/g). Weigh 1g (accurate to 0.1mg) after drying fruit after 2h 96 ℃ ± 2 ℃
Sugar, dextrose, sucrose standards in 50mL volumetric flask, dissolved in water and constant volume. Solution was placed at 4 ℃, can be stored for a month.
4.7 series of standard working solution of sugar. sugar series Imbibe were standard stock solution (4.6) 0.00mL, 0.05mL, 0.25mL, 0.50mL,
1mL, 2.50mL, 5.00mL to 10mL volumetric flask, dissolved in water and constant volume, respectively, equivalent to 0.0mg/g, 0.1mg/g,
0.5mg/g, 1.0mg/g, 2.0mg/g, 5.0mg/g, 10.0mg/g concentration of the standard.
5 Equipment
5.1 High performance liquid chromatograph. with a differential refractive index detector or evaporative light scattering detector.
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