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GB/T 27981-2011: Real-time PCR method for the detection of infectious bovine rhinotracheitis virus
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| GB/T 27981-2011 | English | 229 |
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Real-time PCR method for the detection of infectious bovine rhinotracheitis virus
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GB/T 27981-2011
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Basic data | Standard ID | GB/T 27981-2011 (GB/T27981-2011) | | Description (Translated English) | Real-time PCR method for the detection of infectious bovine rhinotracheitis virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,155 | | Date of Issue | 2011-12-30 | | Date of Implementation | 2012-06-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Announcement of Newly Approved National Standards No. 23 of 2011 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the infectious bovine rhinotracheitis virus (bovine herpes virus type I, BoHV-1) real-time PCR detection method of the technical requirements. This standard applies to quick detection of bovine blood samples, swabs, semen, animal tissue (mucosa, liver, spleen, kidney, lymph nodes, placenta, etc.) and other cell culture samples BoHV-1 infection. | GB/T 27981-2011: Real-time PCR method for the detection of infectious bovine rhinotracheitis virus
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Real-time PCR method for the detection of infectious bovine rhinotracheitis virus
ICS 11.220
B41
National Standards of People's Republic of China
Real-time infectious bovine rhinotracheitis virus
PCR assay method
Issued on. 2011-12-30
2012-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard reference OIE's "terrestrial animal diagnostic tests and vaccines Handbook" (2008 Edition) on infectious bovine rhinotracheitis/Cattle Biography
Stained purulent vulvovaginitis (Chapter2.4.13) content.
This standard by the National Standardization Technical Committee Animal Epidemic Prevention (SAC/TC181) centralized.
This standard was drafted. People's Republic of China Guangdong CIQ, People's Republic of China Jiangsu Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Chen Ru, Liu Zhongyong, Lin Zhixiong, Luo Qiong, ZENG Bi Jian, such as Su Xu, Jiang Yan, Wu Xiaowei.
Real-time infectious bovine rhinotracheitis virus
PCR assay method
1 Scope
This standard specifies the infectious bovine rhinotracheitis virus (bovine herpesvirus type I, BoHV-1) real-time PCR technology to detect fluorescence method
Claim.
This standard applies to quick detection of bovine blood samples, swabs, semen, animal tissues (mucosa, liver, spleen, kidney, lymph node, placenta, etc.) and cell
Cultures and other samples BoHV-1 infection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
World Organisation for Animal Health (OIE) "Terrestrial animal diagnostic tests and vaccines Standards Manual" (2008 edition) for the detection of frozen bovine semen
Real-time PCR method BoHV-1 virus
3 Abbreviations
The following abbreviations apply to this document.
BoHV-1. bovineherpesvirustype1, bovine herpes virus type I.
Fluorescent signal cycle number reaches a set threshold when experienced. Ct value.
DNA. deoxyribonucleicacid, DNA.
DTT. DL-dithiothreitol, dithiothreitol.
EDTA. ethylenediaminetetraaceticacid, ethylenediaminetetraacetic acid.
IBR. bovineinfectiousrhinotracheitis, infectious bovine rhinotracheitis.
PBS. phosphatebuffersolution, phosphate buffered saline.
PCR. polymerasechainreaction, polymerase chain reaction.
SDS. sodiumdodecylsulfate, sodium dodecyl sulfate.
UDG enzyme. uracilDNAglycosylase, uracil DNA glycosylase.
4 installations and equipment
4.1 fluorescent PCR instrument.
4.2 desktop refrigerated centrifuge.
Adjustable micropipette 4.3. Specifications 10μL, 100μL, 200μL, 1mL.
4.4 with a trace filter tip. Specifications 10μL, 100μL, 200μL, 1mL.
4.5 PCR reaction tube optics.
4.6 microcentrifuge tube.
4.7 Vortex device.
4.8 incubator.
4.9 Refrigerator. Specification 2 ℃ ~ 8 ℃, -20 ℃, -80 ℃.
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